Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00400 and BPHYT_RS00405 are separated by 17 nucleotides BPHYT_RS00405 and BPHYT_RS00410 are separated by 27 nucleotides BPHYT_RS00410 and BPHYT_RS00415 are separated by 173 nucleotides
BPHYT_RS00400: BPHYT_RS00400 - hypothetical protein, at 101,029 to 101,316
_RS00400
BPHYT_RS00405: BPHYT_RS00405 - hypothetical protein, at 101,334 to 101,687
_RS00405
BPHYT_RS00410: BPHYT_RS00410 - LacI family transcription regulator, at 101,715 to 102,809
_RS00410
BPHYT_RS00415: BPHYT_RS00415 - sugar ABC transporter substrate-binding protein, at 102,983 to 104,251
_RS00415
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1
2 at 100.985 kb on - strand at 101.083 kb on - strand, within BPHYT_RS00400 at 101.132 kb on + strand, within BPHYT_RS00400 at 101.133 kb on - strand, within BPHYT_RS00400 at 101.177 kb on - strand, within BPHYT_RS00400 at 101.308 kb on - strand at 101.339 kb on + strand at 101.340 kb on - strand at 101.340 kb on - strand at 101.371 kb on + strand, within BPHYT_RS00405 at 101.372 kb on - strand, within BPHYT_RS00405 at 101.372 kb on - strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.374 kb on - strand, within BPHYT_RS00405 at 101.374 kb on - strand, within BPHYT_RS00405 at 101.374 kb on - strand, within BPHYT_RS00405 at 101.513 kb on + strand, within BPHYT_RS00405 at 101.513 kb on + strand, within BPHYT_RS00405 at 101.514 kb on - strand, within BPHYT_RS00405 at 101.615 kb on + strand, within BPHYT_RS00405 at 101.615 kb on + strand, within BPHYT_RS00405 at 101.615 kb on + strand, within BPHYT_RS00405 at 101.616 kb on - strand, within BPHYT_RS00405 at 101.616 kb on - strand, within BPHYT_RS00405 at 102.066 kb on + strand, within BPHYT_RS00410 at 102.268 kb on + strand, within BPHYT_RS00410 at 102.272 kb on + strand, within BPHYT_RS00410 at 102.272 kb on + strand, within BPHYT_RS00410 at 102.273 kb on - strand, within BPHYT_RS00410 at 102.273 kb on - strand, within BPHYT_RS00410 at 102.273 kb on - strand, within BPHYT_RS00410 at 102.371 kb on - strand, within BPHYT_RS00410 at 102.568 kb on + strand, within BPHYT_RS00410 at 102.569 kb on - strand, within BPHYT_RS00410 at 102.709 kb on - strand at 103.379 kb on + strand, within BPHYT_RS00415 at 103.391 kb on + strand, within BPHYT_RS00415 at 103.392 kb on - strand, within BPHYT_RS00415 at 103.392 kb on - strand, within BPHYT_RS00415 at 103.392 kb on - strand, within BPHYT_RS00415 at 103.707 kb on - strand, within BPHYT_RS00415 at 103.709 kb on - strand, within BPHYT_RS00415
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 100,985 - +0.1 101,083 - BPHYT_RS00400 0.19 +0.3 101,132 + BPHYT_RS00400 0.36 -0.2 101,133 - BPHYT_RS00400 0.36 +1.5 101,177 - BPHYT_RS00400 0.51 +1.6 101,308 - +0.1 101,339 + +1.8 101,340 - -2.5 101,340 - -0.1 101,371 + BPHYT_RS00405 0.10 -0.1 101,372 - BPHYT_RS00405 0.11 -0.5 101,372 - BPHYT_RS00405 0.11 +0.6 101,373 + BPHYT_RS00405 0.11 +2.6 101,373 + BPHYT_RS00405 0.11 +0.1 101,373 + BPHYT_RS00405 0.11 +0.6 101,373 + BPHYT_RS00405 0.11 -0.3 101,374 - BPHYT_RS00405 0.11 -0.3 101,374 - BPHYT_RS00405 0.11 -0.0 101,374 - BPHYT_RS00405 0.11 -0.2 101,513 + BPHYT_RS00405 0.51 -0.0 101,513 + BPHYT_RS00405 0.51 -0.3 101,514 - BPHYT_RS00405 0.51 -0.0 101,615 + BPHYT_RS00405 0.79 +0.6 101,615 + BPHYT_RS00405 0.79 -0.5 101,615 + BPHYT_RS00405 0.79 +0.4 101,616 - BPHYT_RS00405 0.80 +0.1 101,616 - BPHYT_RS00405 0.80 -0.3 102,066 + BPHYT_RS00410 0.32 -0.3 102,268 + BPHYT_RS00410 0.51 +0.7 102,272 + BPHYT_RS00410 0.51 -0.4 102,272 + BPHYT_RS00410 0.51 +0.5 102,273 - BPHYT_RS00410 0.51 -0.2 102,273 - BPHYT_RS00410 0.51 -0.8 102,273 - BPHYT_RS00410 0.51 -0.5 102,371 - BPHYT_RS00410 0.60 -0.2 102,568 + BPHYT_RS00410 0.78 -0.2 102,569 - BPHYT_RS00410 0.78 -0.4 102,709 - +0.6 103,379 + BPHYT_RS00415 0.31 -1.7 103,391 + BPHYT_RS00415 0.32 -0.3 103,392 - BPHYT_RS00415 0.32 +0.6 103,392 - BPHYT_RS00415 0.32 +0.5 103,392 - BPHYT_RS00415 0.32 -1.1 103,707 - BPHYT_RS00415 0.57 +0.0 103,709 - BPHYT_RS00415 0.57 -0.5
Or see this region's nucleotide sequence