Experiment: CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19640 and BBR_RS21120 are separated by 270 nucleotides BBR_RS21120 and BBR_RS19645 overlap by 60 nucleotides
BBR_RS19640: BBR_RS19640 - phosphoesterase, at 2,189,277 to 2,190,620
_RS19640
BBR_RS21120: BBR_RS21120 - hypothetical protein, at 2,190,891 to 2,191,211
_RS21120
BBR_RS19645: BBR_RS19645 - RNA degradosome polyphosphate kinase, at 2,191,152 to 2,193,389
_RS19645
Position (kb)
2190
2191
2192 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2190.201 kb on + strand, within BBR_RS19640 at 2190.579 kb on - strand at 2190.606 kb on + strand at 2190.621 kb on + strand at 2190.621 kb on + strand at 2190.621 kb on + strand at 2190.623 kb on + strand at 2190.626 kb on + strand at 2190.626 kb on + strand at 2190.629 kb on - strand at 2190.631 kb on - strand at 2190.634 kb on - strand at 2190.683 kb on - strand at 2190.688 kb on - strand at 2190.692 kb on - strand at 2190.699 kb on + strand at 2190.706 kb on + strand at 2190.707 kb on - strand at 2190.721 kb on + strand at 2190.745 kb on + strand at 2190.747 kb on + strand at 2190.755 kb on + strand at 2190.755 kb on + strand at 2190.757 kb on + strand at 2190.757 kb on - strand at 2190.772 kb on - strand at 2190.783 kb on - strand at 2190.790 kb on - strand at 2190.790 kb on - strand at 2190.792 kb on + strand at 2190.792 kb on + strand at 2190.792 kb on + strand at 2190.795 kb on - strand at 2190.795 kb on - strand at 2190.800 kb on - strand at 2190.810 kb on + strand at 2190.826 kb on - strand at 2190.860 kb on + strand at 2190.873 kb on - strand at 2190.941 kb on + strand, within BBR_RS21120 at 2190.948 kb on + strand, within BBR_RS21120 at 2190.948 kb on + strand, within BBR_RS21120 at 2190.956 kb on - strand, within BBR_RS21120 at 2190.968 kb on + strand, within BBR_RS21120 at 2190.968 kb on + strand, within BBR_RS21120 at 2190.979 kb on + strand, within BBR_RS21120 at 2191.007 kb on - strand, within BBR_RS21120 at 2191.007 kb on - strand, within BBR_RS21120 at 2191.013 kb on + strand, within BBR_RS21120 at 2191.044 kb on + strand, within BBR_RS21120 at 2191.062 kb on + strand, within BBR_RS21120 at 2191.062 kb on + strand, within BBR_RS21120 at 2191.068 kb on - strand, within BBR_RS21120 at 2191.080 kb on - strand, within BBR_RS21120 at 2191.109 kb on + strand, within BBR_RS21120 at 2191.109 kb on + strand, within BBR_RS21120 at 2191.118 kb on - strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.129 kb on + strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.137 kb on - strand, within BBR_RS21120 at 2191.151 kb on - strand, within BBR_RS21120 at 2191.175 kb on + strand at 2191.175 kb on + strand at 2191.175 kb on + strand at 2191.175 kb on + strand at 2191.175 kb on + strand at 2191.175 kb on + strand at 2191.180 kb on + strand at 2191.185 kb on + strand at 2191.208 kb on + strand at 2191.217 kb on - strand at 2191.268 kb on + strand at 2191.431 kb on + strand, within BBR_RS19645 at 2191.441 kb on + strand, within BBR_RS19645 at 2191.452 kb on + strand, within BBR_RS19645 at 2191.456 kb on + strand, within BBR_RS19645 at 2191.510 kb on + strand, within BBR_RS19645 at 2191.571 kb on - strand, within BBR_RS19645 at 2191.621 kb on - strand, within BBR_RS19645 at 2191.701 kb on - strand, within BBR_RS19645 at 2191.793 kb on + strand, within BBR_RS19645 at 2191.866 kb on + strand, within BBR_RS19645 at 2191.889 kb on + strand, within BBR_RS19645 at 2192.006 kb on - strand, within BBR_RS19645 at 2192.011 kb on - strand, within BBR_RS19645 at 2192.024 kb on - strand, within BBR_RS19645 at 2192.027 kb on + strand, within BBR_RS19645 at 2192.051 kb on - strand, within BBR_RS19645 at 2192.106 kb on + strand, within BBR_RS19645 at 2192.109 kb on - strand, within BBR_RS19645 at 2192.122 kb on + strand, within BBR_RS19645 at 2192.124 kb on - strand, within BBR_RS19645 at 2192.134 kb on - strand, within BBR_RS19645 at 2192.152 kb on - strand, within BBR_RS19645 at 2192.166 kb on - strand, within BBR_RS19645 at 2192.180 kb on + strand, within BBR_RS19645 at 2192.207 kb on + strand, within BBR_RS19645 at 2192.210 kb on + strand, within BBR_RS19645 at 2192.210 kb on + strand, within BBR_RS19645
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos remove 2,190,201 + BBR_RS19640 0.69 +1.6 2,190,579 - +2.6 2,190,606 + +0.1 2,190,621 + +0.7 2,190,621 + +0.7 2,190,621 + +1.2 2,190,623 + +0.3 2,190,626 + +2.0 2,190,626 + +2.7 2,190,629 - +0.9 2,190,631 - +0.2 2,190,634 - -0.5 2,190,683 - +0.9 2,190,688 - +0.2 2,190,692 - +0.2 2,190,699 + -0.2 2,190,706 + -1.7 2,190,707 - +0.2 2,190,721 + -0.8 2,190,745 + -1.1 2,190,747 + -1.7 2,190,755 + -0.5 2,190,755 + -1.0 2,190,757 + -1.0 2,190,757 - -0.5 2,190,772 - -1.0 2,190,783 - -3.0 2,190,790 - -0.2 2,190,790 - +0.4 2,190,792 + -1.1 2,190,792 + -1.4 2,190,792 + -0.6 2,190,795 - -1.4 2,190,795 - -1.1 2,190,800 - -1.1 2,190,810 + -1.7 2,190,826 - -0.6 2,190,860 + -1.2 2,190,873 - -2.5 2,190,941 + BBR_RS21120 0.16 +0.3 2,190,948 + BBR_RS21120 0.18 +1.5 2,190,948 + BBR_RS21120 0.18 -0.8 2,190,956 - BBR_RS21120 0.20 -2.2 2,190,968 + BBR_RS21120 0.24 -1.2 2,190,968 + BBR_RS21120 0.24 -2.5 2,190,979 + BBR_RS21120 0.27 -0.7 2,191,007 - BBR_RS21120 0.36 -2.9 2,191,007 - BBR_RS21120 0.36 -2.8 2,191,013 + BBR_RS21120 0.38 -2.2 2,191,044 + BBR_RS21120 0.48 -1.0 2,191,062 + BBR_RS21120 0.53 -0.5 2,191,062 + BBR_RS21120 0.53 -2.8 2,191,068 - BBR_RS21120 0.55 +1.7 2,191,080 - BBR_RS21120 0.59 -1.2 2,191,109 + BBR_RS21120 0.68 -1.9 2,191,109 + BBR_RS21120 0.68 -2.8 2,191,118 - BBR_RS21120 0.71 -2.0 2,191,129 + BBR_RS21120 0.74 -2.3 2,191,129 + BBR_RS21120 0.74 -1.5 2,191,129 + BBR_RS21120 0.74 -1.0 2,191,129 + BBR_RS21120 0.74 -1.2 2,191,129 + BBR_RS21120 0.74 -1.8 2,191,129 + BBR_RS21120 0.74 -3.5 2,191,129 + BBR_RS21120 0.74 -3.0 2,191,129 + BBR_RS21120 0.74 -2.5 2,191,129 + BBR_RS21120 0.74 -0.6 2,191,129 + BBR_RS21120 0.74 -1.0 2,191,137 - BBR_RS21120 0.77 -0.7 2,191,137 - BBR_RS21120 0.77 -2.6 2,191,137 - BBR_RS21120 0.77 -1.8 2,191,137 - BBR_RS21120 0.77 -1.0 2,191,137 - BBR_RS21120 0.77 -2.5 2,191,137 - BBR_RS21120 0.77 -1.7 2,191,137 - BBR_RS21120 0.77 -3.6 2,191,137 - BBR_RS21120 0.77 -2.0 2,191,137 - BBR_RS21120 0.77 -1.7 2,191,137 - BBR_RS21120 0.77 -0.6 2,191,137 - BBR_RS21120 0.77 -1.5 2,191,137 - BBR_RS21120 0.77 -1.5 2,191,137 - BBR_RS21120 0.77 -4.5 2,191,137 - BBR_RS21120 0.77 -2.2 2,191,137 - BBR_RS21120 0.77 -2.0 2,191,137 - BBR_RS21120 0.77 -0.1 2,191,137 - BBR_RS21120 0.77 -0.6 2,191,151 - BBR_RS21120 0.81 -2.7 2,191,175 + -1.5 2,191,175 + -2.5 2,191,175 + -3.3 2,191,175 + -2.0 2,191,175 + -3.7 2,191,175 + -0.3 2,191,180 + -2.5 2,191,185 + -3.2 2,191,208 + -3.5 2,191,217 - -3.0 2,191,268 + -1.5 2,191,431 + BBR_RS19645 0.12 -4.1 2,191,441 + BBR_RS19645 0.13 -3.3 2,191,452 + BBR_RS19645 0.13 -3.1 2,191,456 + BBR_RS19645 0.14 -2.0 2,191,510 + BBR_RS19645 0.16 -1.6 2,191,571 - BBR_RS19645 0.19 -2.6 2,191,621 - BBR_RS19645 0.21 +0.5 2,191,701 - BBR_RS19645 0.25 -1.6 2,191,793 + BBR_RS19645 0.29 -3.1 2,191,866 + BBR_RS19645 0.32 -3.5 2,191,889 + BBR_RS19645 0.33 -0.9 2,192,006 - BBR_RS19645 0.38 -2.6 2,192,011 - BBR_RS19645 0.38 -0.3 2,192,024 - BBR_RS19645 0.39 -3.0 2,192,027 + BBR_RS19645 0.39 -2.3 2,192,051 - BBR_RS19645 0.40 -3.5 2,192,106 + BBR_RS19645 0.43 -3.7 2,192,109 - BBR_RS19645 0.43 -1.2 2,192,122 + BBR_RS19645 0.43 -0.0 2,192,124 - BBR_RS19645 0.43 -2.5 2,192,134 - BBR_RS19645 0.44 -2.1 2,192,152 - BBR_RS19645 0.45 -3.7 2,192,166 - BBR_RS19645 0.45 -2.1 2,192,180 + BBR_RS19645 0.46 -3.3 2,192,207 + BBR_RS19645 0.47 -5.6 2,192,210 + BBR_RS19645 0.47 -2.0 2,192,210 + BBR_RS19645 0.47 -3.4
Or see this region's nucleotide sequence