Experiment: CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15340 and BBR_RS20910 are separated by 325 nucleotides BBR_RS20910 and BBR_RS15345 are separated by 111 nucleotides BBR_RS15345 and BBR_RS15350 are separated by 219 nucleotides
BBR_RS15340: BBR_RS15340 - hypothetical protein, at 1,213,785 to 1,214,480
_RS15340
BBR_RS20910: BBR_RS20910 - DUF1829 domain-containing protein, at 1,214,806 to 1,215,123
_RS20910
BBR_RS15345: BBR_RS15345 - transporter, at 1,215,235 to 1,215,678
_RS15345
BBR_RS15350: BBR_RS15350 - plasmid pRiA4b ORF-3 family protein, at 1,215,898 to 1,216,533
_RS15350
Position (kb)
1215
1216 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1214.260 kb on + strand, within BBR_RS15340 at 1214.298 kb on - strand, within BBR_RS15340 at 1214.375 kb on + strand, within BBR_RS15340 at 1214.377 kb on - strand, within BBR_RS15340 at 1214.433 kb on - strand at 1214.489 kb on + strand at 1214.519 kb on + strand at 1214.552 kb on + strand at 1214.564 kb on + strand at 1214.571 kb on + strand at 1214.578 kb on + strand at 1214.604 kb on + strand at 1214.604 kb on + strand at 1214.604 kb on + strand at 1214.604 kb on + strand at 1214.606 kb on + strand at 1214.612 kb on - strand at 1214.675 kb on - strand at 1214.804 kb on + strand at 1214.823 kb on - strand at 1214.887 kb on + strand, within BBR_RS20910 at 1215.008 kb on + strand, within BBR_RS20910 at 1215.057 kb on - strand, within BBR_RS20910 at 1215.180 kb on + strand at 1215.326 kb on + strand, within BBR_RS15345 at 1215.326 kb on + strand, within BBR_RS15345 at 1215.374 kb on - strand, within BBR_RS15345 at 1215.396 kb on + strand, within BBR_RS15345 at 1215.420 kb on + strand, within BBR_RS15345 at 1215.420 kb on + strand, within BBR_RS15345 at 1215.428 kb on - strand, within BBR_RS15345 at 1215.470 kb on - strand, within BBR_RS15345 at 1215.580 kb on - strand, within BBR_RS15345 at 1215.641 kb on + strand at 1215.684 kb on - strand at 1215.686 kb on + strand at 1215.686 kb on + strand at 1215.686 kb on + strand at 1215.694 kb on - strand at 1215.714 kb on + strand at 1215.833 kb on + strand at 1215.855 kb on + strand at 1215.855 kb on + strand at 1215.894 kb on + strand at 1215.904 kb on + strand at 1216.037 kb on + strand, within BBR_RS15350 at 1216.089 kb on + strand, within BBR_RS15350 at 1216.357 kb on + strand, within BBR_RS15350 at 1216.366 kb on + strand, within BBR_RS15350 at 1216.374 kb on - strand, within BBR_RS15350 at 1216.504 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 with Glycine Leucine Cysteine Methionine Glutamate as the only aminos remove 1,214,260 + BBR_RS15340 0.68 -0.8 1,214,298 - BBR_RS15340 0.74 -0.4 1,214,375 + BBR_RS15340 0.85 +0.5 1,214,377 - BBR_RS15340 0.85 -2.8 1,214,433 - -1.2 1,214,489 + -1.3 1,214,519 + -0.7 1,214,552 + -1.0 1,214,564 + +0.6 1,214,571 + +0.1 1,214,578 + -0.8 1,214,604 + -3.6 1,214,604 + -1.0 1,214,604 + -1.3 1,214,604 + -0.8 1,214,606 + -0.8 1,214,612 - -1.8 1,214,675 - -1.0 1,214,804 + +0.2 1,214,823 - -1.6 1,214,887 + BBR_RS20910 0.25 -1.5 1,215,008 + BBR_RS20910 0.64 +0.4 1,215,057 - BBR_RS20910 0.79 -2.2 1,215,180 + +0.5 1,215,326 + BBR_RS15345 0.20 -1.5 1,215,326 + BBR_RS15345 0.20 -2.1 1,215,374 - BBR_RS15345 0.31 -2.8 1,215,396 + BBR_RS15345 0.36 -0.4 1,215,420 + BBR_RS15345 0.42 -1.2 1,215,420 + BBR_RS15345 0.42 +0.1 1,215,428 - BBR_RS15345 0.43 -1.0 1,215,470 - BBR_RS15345 0.53 -2.7 1,215,580 - BBR_RS15345 0.78 -0.3 1,215,641 + +0.6 1,215,684 - -1.7 1,215,686 + -0.7 1,215,686 + -2.2 1,215,686 + -1.0 1,215,694 - -0.3 1,215,714 + -0.9 1,215,833 + +0.5 1,215,855 + -0.8 1,215,855 + -0.3 1,215,894 + -0.9 1,215,904 + -0.6 1,216,037 + BBR_RS15350 0.22 -0.9 1,216,089 + BBR_RS15350 0.30 -0.6 1,216,357 + BBR_RS15350 0.72 +1.0 1,216,366 + BBR_RS15350 0.74 -0.4 1,216,374 - BBR_RS15350 0.75 -2.6 1,216,504 + +0.2
Or see this region's nucleotide sequence