Strain Fitness in Rahnella sp. WP5 around EX31_RS22145

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS22140 and EX31_RS22145 are separated by 62 nucleotidesEX31_RS22145 and EX31_RS22150 are separated by 69 nucleotides EX31_RS22140: EX31_RS22140 - thiamine-phosphate kinase, at 175,909 to 176,895 _RS22140 EX31_RS22145: EX31_RS22145 - phosphatidylglycerophosphatase A, at 176,958 to 177,452 _RS22145 EX31_RS22150: EX31_RS22150 - aldo/keto reductase, at 177,522 to 178,496 _RS22150 Position (kb) 176 177 178Strain fitness (log2 ratio) -2 -1 0 1 2at 176.906 kb on + strandat 177.031 kb on + strand, within EX31_RS22145at 177.096 kb on + strand, within EX31_RS22145at 177.096 kb on + strand, within EX31_RS22145at 177.137 kb on + strand, within EX31_RS22145at 177.208 kb on + strand, within EX31_RS22145at 177.414 kb on + strandat 177.464 kb on + strandat 177.465 kb on - strandat 177.467 kb on + strandat 177.467 kb on + strandat 177.468 kb on - strandat 177.470 kb on + strandat 177.470 kb on + strandat 177.518 kb on + strandat 177.519 kb on - strandat 177.520 kb on + strandat 177.520 kb on + strandat 177.521 kb on - strandat 177.553 kb on + strandat 177.553 kb on + strandat 177.553 kb on + strandat 177.554 kb on - strandat 177.554 kb on - strandat 177.554 kb on - strandat 177.554 kb on - strandat 177.554 kb on - strandat 177.554 kb on - strandat 177.778 kb on + strand, within EX31_RS22150at 177.779 kb on - strand, within EX31_RS22150at 177.779 kb on - strand, within EX31_RS22150at 177.814 kb on - strand, within EX31_RS22150at 177.814 kb on - strand, within EX31_RS22150at 177.814 kb on - strand, within EX31_RS22150at 177.873 kb on + strand, within EX31_RS22150at 177.874 kb on - strand, within EX31_RS22150at 177.904 kb on + strand, within EX31_RS22150at 177.904 kb on + strand, within EX31_RS22150at 177.905 kb on - strand, within EX31_RS22150at 177.940 kb on + strand, within EX31_RS22150at 177.941 kb on - strand, within EX31_RS22150at 177.941 kb on - strand, within EX31_RS22150at 177.941 kb on - strand, within EX31_RS22150at 178.038 kb on - strand, within EX31_RS22150at 178.139 kb on - strand, within EX31_RS22150at 178.139 kb on - strand, within EX31_RS22150at 178.139 kb on - strand, within EX31_RS22150at 178.139 kb on - strand, within EX31_RS22150at 178.139 kb on - strand, within EX31_RS22150at 178.229 kb on - strand, within EX31_RS22150at 178.316 kb on - strand, within EX31_RS22150at 178.316 kb on - strand, within EX31_RS22150at 178.340 kb on + strand, within EX31_RS22150at 178.341 kb on - strand, within EX31_RS22150at 178.341 kb on - strand, within EX31_RS22150at 178.437 kb on - strandat 178.437 kb on - strandat 178.437 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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176,906 + -0.0
177,031 + EX31_RS22145 0.15 +0.3
177,096 + EX31_RS22145 0.28 -2.2
177,096 + EX31_RS22145 0.28 -1.8
177,137 + EX31_RS22145 0.36 -1.0
177,208 + EX31_RS22145 0.51 +1.0
177,414 + -2.1
177,464 + +0.3
177,465 - +1.1
177,467 + +0.4
177,467 + +1.0
177,468 - -1.0
177,470 + -1.2
177,470 + +0.8
177,518 + +1.0
177,519 - -1.7
177,520 + -1.8
177,520 + -0.3
177,521 - -1.1
177,553 + +2.0
177,553 + +1.2
177,553 + -2.3
177,554 - -1.6
177,554 - +0.9
177,554 - -0.3
177,554 - +2.0
177,554 - +1.1
177,554 - -1.0
177,778 + EX31_RS22150 0.26 +0.7
177,779 - EX31_RS22150 0.26 -0.5
177,779 - EX31_RS22150 0.26 -0.3
177,814 - EX31_RS22150 0.30 +2.0
177,814 - EX31_RS22150 0.30 +1.3
177,814 - EX31_RS22150 0.30 -0.0
177,873 + EX31_RS22150 0.36 +2.0
177,874 - EX31_RS22150 0.36 -0.3
177,904 + EX31_RS22150 0.39 +1.0
177,904 + EX31_RS22150 0.39 +1.4
177,905 - EX31_RS22150 0.39 +0.4
177,940 + EX31_RS22150 0.43 +1.0
177,941 - EX31_RS22150 0.43 +2.3
177,941 - EX31_RS22150 0.43 -0.0
177,941 - EX31_RS22150 0.43 +2.6
178,038 - EX31_RS22150 0.53 -0.1
178,139 - EX31_RS22150 0.63 +1.0
178,139 - EX31_RS22150 0.63 -2.5
178,139 - EX31_RS22150 0.63 +0.2
178,139 - EX31_RS22150 0.63 -1.6
178,139 - EX31_RS22150 0.63 +1.0
178,229 - EX31_RS22150 0.73 -0.3
178,316 - EX31_RS22150 0.81 -0.9
178,316 - EX31_RS22150 0.81 +0.8
178,340 + EX31_RS22150 0.84 -1.0
178,341 - EX31_RS22150 0.84 +1.5
178,341 - EX31_RS22150 0.84 -1.0
178,437 - -2.5
178,437 - +1.0
178,437 - +0.4

Or see this region's nucleotide sequence