Strain Fitness in Rahnella sp. WP5 around EX31_RS19420

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS19415 and EX31_RS19420 overlap by 4 nucleotidesEX31_RS19420 and EX31_RS19425 are separated by 38 nucleotides EX31_RS19415: EX31_RS19415 - NADH-quinone oxidoreductase subunit NuoF, at 511,368 to 512,729 _RS19415 EX31_RS19420: EX31_RS19420 - NADH-quinone oxidoreductase subunit NuoE, at 512,726 to 513,226 _RS19420 EX31_RS19425: EX31_RS19425 - NADH-quinone oxidoreductase subunit C/D, at 513,265 to 515,064 _RS19425 Position (kb) 512 513 514Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 511.758 kb on - strand, within EX31_RS19415at 512.003 kb on + strand, within EX31_RS19415at 512.012 kb on - strand, within EX31_RS19415at 512.133 kb on - strand, within EX31_RS19415at 512.247 kb on - strand, within EX31_RS19415at 512.310 kb on - strand, within EX31_RS19415at 512.421 kb on - strand, within EX31_RS19415at 512.422 kb on + strand, within EX31_RS19415at 512.422 kb on + strand, within EX31_RS19415at 512.422 kb on + strand, within EX31_RS19415at 512.423 kb on - strand, within EX31_RS19415at 512.423 kb on - strand, within EX31_RS19415at 512.423 kb on - strand, within EX31_RS19415at 512.423 kb on - strand, within EX31_RS19415at 512.451 kb on - strand, within EX31_RS19415at 512.721 kb on - strandat 512.722 kb on + strandat 512.722 kb on + strandat 512.722 kb on + strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 512.723 kb on - strandat 513.000 kb on + strand, within EX31_RS19420at 513.001 kb on - strand, within EX31_RS19420at 513.001 kb on - strand, within EX31_RS19420at 513.061 kb on - strand, within EX31_RS19420at 513.064 kb on + strand, within EX31_RS19420at 513.065 kb on - strand, within EX31_RS19420at 513.065 kb on - strand, within EX31_RS19420at 513.142 kb on - strand, within EX31_RS19420at 513.142 kb on - strand, within EX31_RS19420at 513.229 kb on - strandat 513.229 kb on - strandat 513.286 kb on + strandat 513.286 kb on + strandat 513.287 kb on - strandat 513.287 kb on - strandat 513.287 kb on - strandat 513.309 kb on - strandat 513.399 kb on - strandat 513.428 kb on + strandat 513.428 kb on + strandat 513.429 kb on - strandat 513.429 kb on - strandat 513.431 kb on + strandat 513.432 kb on - strandat 513.656 kb on - strand, within EX31_RS19425at 513.656 kb on - strand, within EX31_RS19425at 513.678 kb on - strand, within EX31_RS19425at 513.750 kb on + strand, within EX31_RS19425at 513.751 kb on - strand, within EX31_RS19425at 513.751 kb on - strand, within EX31_RS19425at 513.827 kb on + strand, within EX31_RS19425at 513.827 kb on + strand, within EX31_RS19425at 513.887 kb on + strand, within EX31_RS19425at 513.887 kb on + strand, within EX31_RS19425at 513.887 kb on + strand, within EX31_RS19425at 513.888 kb on - strand, within EX31_RS19425at 514.103 kb on + strand, within EX31_RS19425at 514.104 kb on - strand, within EX31_RS19425at 514.123 kb on - strand, within EX31_RS19425at 514.209 kb on - strand, within EX31_RS19425at 514.209 kb on - strand, within EX31_RS19425at 514.209 kb on - strand, within EX31_RS19425

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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511,758 - EX31_RS19415 0.29 -1.3
512,003 + EX31_RS19415 0.47 +1.6
512,012 - EX31_RS19415 0.47 -0.6
512,133 - EX31_RS19415 0.56 +0.8
512,247 - EX31_RS19415 0.65 -2.0
512,310 - EX31_RS19415 0.69 -2.3
512,421 - EX31_RS19415 0.77 +1.0
512,422 + EX31_RS19415 0.77 -0.9
512,422 + EX31_RS19415 0.77 +1.0
512,422 + EX31_RS19415 0.77 +0.0
512,423 - EX31_RS19415 0.77 -2.2
512,423 - EX31_RS19415 0.77 +1.0
512,423 - EX31_RS19415 0.77 +0.0
512,423 - EX31_RS19415 0.77 -0.6
512,451 - EX31_RS19415 0.80 -1.0
512,721 - -1.0
512,722 + +1.4
512,722 + -0.6
512,722 + +2.6
512,723 - -1.0
512,723 - +1.4
512,723 - +1.3
512,723 - +0.0
512,723 - -0.6
512,723 - +1.4
512,723 - -1.0
512,723 - -1.6
512,723 - -1.8
512,723 - -0.7
512,723 - +0.0
513,000 + EX31_RS19420 0.55 -0.6
513,001 - EX31_RS19420 0.55 -3.0
513,001 - EX31_RS19420 0.55 -1.6
513,061 - EX31_RS19420 0.67 +0.0
513,064 + EX31_RS19420 0.67 +0.0
513,065 - EX31_RS19420 0.68 +0.0
513,065 - EX31_RS19420 0.68 +0.0
513,142 - EX31_RS19420 0.83 -0.2
513,142 - EX31_RS19420 0.83 -1.3
513,229 - +0.0
513,229 - -1.0
513,286 + +0.0
513,286 + -1.6
513,287 - +1.0
513,287 - +1.0
513,287 - +1.0
513,309 - -1.3
513,399 - +0.0
513,428 + +0.4
513,428 + +0.0
513,429 - +2.0
513,429 - -0.6
513,431 + -1.0
513,432 - -1.3
513,656 - EX31_RS19425 0.22 -0.6
513,656 - EX31_RS19425 0.22 -1.3
513,678 - EX31_RS19425 0.23 +1.0
513,750 + EX31_RS19425 0.27 -0.6
513,751 - EX31_RS19425 0.27 -0.6
513,751 - EX31_RS19425 0.27 +2.0
513,827 + EX31_RS19425 0.31 -1.0
513,827 + EX31_RS19425 0.31 -0.4
513,887 + EX31_RS19425 0.35 +0.0
513,887 + EX31_RS19425 0.35 -0.6
513,887 + EX31_RS19425 0.35 +1.0
513,888 - EX31_RS19425 0.35 -0.9
514,103 + EX31_RS19425 0.47 -1.3
514,104 - EX31_RS19425 0.47 +0.0
514,123 - EX31_RS19425 0.48 -1.3
514,209 - EX31_RS19425 0.52 +0.6
514,209 - EX31_RS19425 0.52 -1.0
514,209 - EX31_RS19425 0.52 +0.0

Or see this region's nucleotide sequence