Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19065 and EX31_RS19070 are separated by 15 nucleotides EX31_RS19070 and EX31_RS19075 overlap by 4 nucleotides EX31_RS19075 and EX31_RS19080 are separated by 463 nucleotides
EX31_RS19065: EX31_RS19065 - PTS fructose transporter subunit IIBC, at 404,665 to 406,365
_RS19065
EX31_RS19070: EX31_RS19070 - 1-phosphofructokinase, at 406,381 to 407,316
_RS19070
EX31_RS19075: EX31_RS19075 - fused PTS fructose transporter subunit IIA/HPr protein, at 407,313 to 408,443
_RS19075
EX31_RS19080: EX31_RS19080 - sugar efflux transporter, at 408,907 to 410,121
_RS19080
Position (kb) 407
408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 406.345 kb on + strand at 406.346 kb on - strand at 406.346 kb on - strand at 406.346 kb on - strand at 406.346 kb on - strand at 406.383 kb on - strand at 406.383 kb on - strand at 406.450 kb on - strand at 406.493 kb on - strand, within EX31_RS19070 at 406.497 kb on + strand, within EX31_RS19070 at 406.497 kb on + strand, within EX31_RS19070 at 406.497 kb on + strand, within EX31_RS19070 at 406.497 kb on + strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.498 kb on - strand, within EX31_RS19070 at 406.529 kb on + strand, within EX31_RS19070 at 406.530 kb on - strand, within EX31_RS19070 at 406.530 kb on - strand, within EX31_RS19070 at 406.530 kb on - strand, within EX31_RS19070 at 406.530 kb on - strand, within EX31_RS19070 at 406.699 kb on + strand, within EX31_RS19070 at 406.700 kb on - strand, within EX31_RS19070 at 406.759 kb on - strand, within EX31_RS19070 at 407.043 kb on + strand, within EX31_RS19070 at 407.099 kb on - strand, within EX31_RS19070 at 407.188 kb on - strand, within EX31_RS19070 at 407.188 kb on - strand, within EX31_RS19070 at 407.197 kb on - strand, within EX31_RS19070 at 407.225 kb on - strand at 407.276 kb on + strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.293 kb on - strand at 407.296 kb on - strand at 407.362 kb on - strand at 407.362 kb on - strand at 407.514 kb on - strand, within EX31_RS19075 at 407.516 kb on + strand, within EX31_RS19075 at 407.517 kb on - strand, within EX31_RS19075 at 407.575 kb on + strand, within EX31_RS19075 at 407.575 kb on + strand, within EX31_RS19075 at 407.575 kb on + strand, within EX31_RS19075 at 407.576 kb on - strand, within EX31_RS19075 at 407.576 kb on - strand, within EX31_RS19075 at 407.576 kb on - strand, within EX31_RS19075 at 407.576 kb on - strand, within EX31_RS19075 at 407.576 kb on - strand, within EX31_RS19075 at 407.576 kb on - strand, within EX31_RS19075 at 407.576 kb on - strand, within EX31_RS19075 at 407.695 kb on + strand, within EX31_RS19075 at 407.696 kb on - strand, within EX31_RS19075 at 407.696 kb on - strand, within EX31_RS19075 at 407.696 kb on - strand, within EX31_RS19075 at 407.696 kb on - strand, within EX31_RS19075 at 407.696 kb on - strand, within EX31_RS19075 at 407.867 kb on + strand, within EX31_RS19075 at 407.867 kb on + strand, within EX31_RS19075 at 407.868 kb on - strand, within EX31_RS19075 at 408.094 kb on - strand, within EX31_RS19075 at 408.094 kb on - strand, within EX31_RS19075 at 408.163 kb on + strand, within EX31_RS19075 at 408.163 kb on + strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.164 kb on - strand, within EX31_RS19075 at 408.189 kb on - strand, within EX31_RS19075 at 408.189 kb on - strand, within EX31_RS19075 at 408.246 kb on - strand, within EX31_RS19075 at 408.286 kb on + strand, within EX31_RS19075 at 408.286 kb on + strand, within EX31_RS19075 at 408.287 kb on - strand, within EX31_RS19075 at 408.287 kb on - strand, within EX31_RS19075 at 408.287 kb on - strand, within EX31_RS19075 at 408.287 kb on - strand, within EX31_RS19075 at 408.287 kb on - strand, within EX31_RS19075 at 408.287 kb on - strand, within EX31_RS19075 at 408.287 kb on - strand, within EX31_RS19075 at 408.290 kb on + strand, within EX31_RS19075 at 408.290 kb on + strand, within EX31_RS19075 at 408.290 kb on + strand, within EX31_RS19075 at 408.291 kb on - strand, within EX31_RS19075 at 408.291 kb on - strand, within EX31_RS19075 at 408.291 kb on - strand, within EX31_RS19075 at 408.291 kb on - strand, within EX31_RS19075 at 408.291 kb on - strand, within EX31_RS19075 at 408.291 kb on - strand, within EX31_RS19075 at 408.324 kb on + strand, within EX31_RS19075 at 408.324 kb on + strand, within EX31_RS19075 at 408.325 kb on - strand, within EX31_RS19075 at 408.325 kb on - strand, within EX31_RS19075 at 408.328 kb on + strand, within EX31_RS19075 at 408.329 kb on - strand, within EX31_RS19075 at 408.380 kb on + strand at 408.419 kb on + strand at 408.419 kb on + strand at 408.420 kb on - strand at 408.443 kb on + strand at 408.444 kb on - strand at 408.444 kb on - strand at 408.444 kb on - strand at 408.444 kb on - strand at 408.444 kb on - strand at 408.444 kb on - strand at 408.444 kb on - strand at 408.570 kb on - strand at 408.570 kb on - strand at 408.612 kb on + strand at 408.612 kb on + strand at 408.613 kb on - strand at 408.779 kb on - strand at 408.856 kb on + strand at 408.856 kb on + strand at 408.886 kb on + strand at 408.887 kb on - strand at 408.887 kb on - strand at 408.901 kb on + strand at 408.901 kb on + strand at 408.901 kb on + strand at 408.901 kb on + strand at 408.902 kb on - strand at 408.902 kb on - strand at 408.902 kb on - strand at 408.902 kb on - strand at 408.902 kb on - strand at 408.902 kb on - strand at 408.906 kb on - strand at 409.056 kb on + strand, within EX31_RS19080 at 409.056 kb on + strand, within EX31_RS19080 at 409.057 kb on - strand, within EX31_RS19080 at 409.057 kb on - strand, within EX31_RS19080 at 409.057 kb on - strand, within EX31_RS19080 at 409.066 kb on - strand, within EX31_RS19080 at 409.162 kb on + strand, within EX31_RS19080 at 409.162 kb on + strand, within EX31_RS19080 at 409.206 kb on + strand, within EX31_RS19080 at 409.212 kb on + strand, within EX31_RS19080 at 409.212 kb on + strand, within EX31_RS19080 at 409.216 kb on + strand, within EX31_RS19080 at 409.216 kb on + strand, within EX31_RS19080 at 409.217 kb on - strand, within EX31_RS19080 at 409.217 kb on - strand, within EX31_RS19080 at 409.217 kb on - strand, within EX31_RS19080 at 409.222 kb on - strand, within EX31_RS19080 at 409.289 kb on + strand, within EX31_RS19080 at 409.425 kb on + strand, within EX31_RS19080 at 409.425 kb on + strand, within EX31_RS19080 at 409.425 kb on + strand, within EX31_RS19080 at 409.425 kb on + strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.426 kb on - strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.429 kb on + strand, within EX31_RS19080 at 409.430 kb on - strand, within EX31_RS19080 at 409.430 kb on - strand, within EX31_RS19080 at 409.430 kb on - strand, within EX31_RS19080
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 406,345 + +1.7 406,346 - +0.7 406,346 - -0.8 406,346 - -0.3 406,346 - +2.8 406,383 - +0.7 406,383 - -0.6 406,450 - +0.4 406,493 - EX31_RS19070 0.12 +0.3 406,497 + EX31_RS19070 0.12 -0.6 406,497 + EX31_RS19070 0.12 +1.0 406,497 + EX31_RS19070 0.12 +2.4 406,497 + EX31_RS19070 0.12 +2.0 406,498 - EX31_RS19070 0.12 -1.0 406,498 - EX31_RS19070 0.12 +0.8 406,498 - EX31_RS19070 0.12 +0.2 406,498 - EX31_RS19070 0.12 -0.2 406,498 - EX31_RS19070 0.12 -0.6 406,498 - EX31_RS19070 0.12 -0.8 406,498 - EX31_RS19070 0.12 -2.5 406,498 - EX31_RS19070 0.12 -0.4 406,498 - EX31_RS19070 0.12 -0.2 406,498 - EX31_RS19070 0.12 +0.1 406,529 + EX31_RS19070 0.16 +1.6 406,530 - EX31_RS19070 0.16 +0.8 406,530 - EX31_RS19070 0.16 +0.8 406,530 - EX31_RS19070 0.16 +0.7 406,530 - EX31_RS19070 0.16 -0.6 406,699 + EX31_RS19070 0.34 +1.0 406,700 - EX31_RS19070 0.34 +0.1 406,759 - EX31_RS19070 0.40 +0.1 407,043 + EX31_RS19070 0.71 -0.0 407,099 - EX31_RS19070 0.77 +0.7 407,188 - EX31_RS19070 0.86 +0.4 407,188 - EX31_RS19070 0.86 -0.4 407,197 - EX31_RS19070 0.87 +0.7 407,225 - +1.1 407,276 + -1.0 407,293 - +1.2 407,293 - +0.6 407,293 - -1.8 407,293 - +0.2 407,293 - -1.8 407,293 - +1.6 407,293 - +0.9 407,293 - +0.4 407,293 - +1.0 407,296 - +1.2 407,362 - +0.5 407,362 - +0.8 407,514 - EX31_RS19075 0.18 +0.3 407,516 + EX31_RS19075 0.18 -0.1 407,517 - EX31_RS19075 0.18 +0.2 407,575 + EX31_RS19075 0.23 +0.4 407,575 + EX31_RS19075 0.23 -0.6 407,575 + EX31_RS19075 0.23 +1.6 407,576 - EX31_RS19075 0.23 -0.0 407,576 - EX31_RS19075 0.23 +1.2 407,576 - EX31_RS19075 0.23 -0.4 407,576 - EX31_RS19075 0.23 -0.0 407,576 - EX31_RS19075 0.23 -0.4 407,576 - EX31_RS19075 0.23 -0.4 407,576 - EX31_RS19075 0.23 -2.6 407,695 + EX31_RS19075 0.34 -1.1 407,696 - EX31_RS19075 0.34 +0.7 407,696 - EX31_RS19075 0.34 -1.3 407,696 - EX31_RS19075 0.34 -0.3 407,696 - EX31_RS19075 0.34 +0.5 407,696 - EX31_RS19075 0.34 +0.2 407,867 + EX31_RS19075 0.49 +0.7 407,867 + EX31_RS19075 0.49 -0.9 407,868 - EX31_RS19075 0.49 -0.3 408,094 - EX31_RS19075 0.69 -0.6 408,094 - EX31_RS19075 0.69 +0.8 408,163 + EX31_RS19075 0.75 +1.7 408,163 + EX31_RS19075 0.75 +0.8 408,164 - EX31_RS19075 0.75 +0.2 408,164 - EX31_RS19075 0.75 -2.8 408,164 - EX31_RS19075 0.75 -0.2 408,164 - EX31_RS19075 0.75 +1.5 408,164 - EX31_RS19075 0.75 +0.3 408,164 - EX31_RS19075 0.75 -0.4 408,164 - EX31_RS19075 0.75 -1.4 408,164 - EX31_RS19075 0.75 +1.0 408,164 - EX31_RS19075 0.75 +1.2 408,164 - EX31_RS19075 0.75 +1.3 408,164 - EX31_RS19075 0.75 +1.1 408,189 - EX31_RS19075 0.77 +0.1 408,189 - EX31_RS19075 0.77 -0.1 408,246 - EX31_RS19075 0.82 -1.3 408,286 + EX31_RS19075 0.86 +0.4 408,286 + EX31_RS19075 0.86 +1.2 408,287 - EX31_RS19075 0.86 +0.3 408,287 - EX31_RS19075 0.86 -0.0 408,287 - EX31_RS19075 0.86 -0.6 408,287 - EX31_RS19075 0.86 +0.4 408,287 - EX31_RS19075 0.86 +1.3 408,287 - EX31_RS19075 0.86 +0.7 408,287 - EX31_RS19075 0.86 +0.1 408,290 + EX31_RS19075 0.86 +0.7 408,290 + EX31_RS19075 0.86 +1.0 408,290 + EX31_RS19075 0.86 -1.3 408,291 - EX31_RS19075 0.86 +0.4 408,291 - EX31_RS19075 0.86 +1.7 408,291 - EX31_RS19075 0.86 +1.2 408,291 - EX31_RS19075 0.86 +1.0 408,291 - EX31_RS19075 0.86 +0.4 408,291 - EX31_RS19075 0.86 -0.4 408,324 + EX31_RS19075 0.89 +1.0 408,324 + EX31_RS19075 0.89 +0.2 408,325 - EX31_RS19075 0.89 +1.1 408,325 - EX31_RS19075 0.89 -2.6 408,328 + EX31_RS19075 0.90 +2.0 408,329 - EX31_RS19075 0.90 +0.6 408,380 + +0.3 408,419 + +0.7 408,419 + +3.8 408,420 - +0.4 408,443 + +0.2 408,444 - +1.0 408,444 - -0.3 408,444 - +1.0 408,444 - +1.6 408,444 - +1.0 408,444 - +0.3 408,444 - -0.0 408,570 - +1.6 408,570 - -1.0 408,612 + +0.3 408,612 + +1.0 408,613 - -1.5 408,779 - -3.3 408,856 + +0.2 408,856 + -0.0 408,886 + -1.6 408,887 - +1.0 408,887 - -0.7 408,901 + -1.8 408,901 + +0.1 408,901 + +0.1 408,901 + +0.9 408,902 - +0.2 408,902 - -0.0 408,902 - +0.8 408,902 - -0.1 408,902 - +3.3 408,902 - +1.1 408,906 - +0.5 409,056 + EX31_RS19080 0.12 -1.2 409,056 + EX31_RS19080 0.12 -0.4 409,057 - EX31_RS19080 0.12 -1.8 409,057 - EX31_RS19080 0.12 -0.0 409,057 - EX31_RS19080 0.12 -1.3 409,066 - EX31_RS19080 0.13 +2.1 409,162 + EX31_RS19080 0.21 -0.6 409,162 + EX31_RS19080 0.21 +0.4 409,206 + EX31_RS19080 0.25 +0.9 409,212 + EX31_RS19080 0.25 -1.0 409,212 + EX31_RS19080 0.25 -2.5 409,216 + EX31_RS19080 0.25 +1.7 409,216 + EX31_RS19080 0.25 -1.6 409,217 - EX31_RS19080 0.26 -0.0 409,217 - EX31_RS19080 0.26 -2.2 409,217 - EX31_RS19080 0.26 -1.3 409,222 - EX31_RS19080 0.26 +1.0 409,289 + EX31_RS19080 0.31 +1.1 409,425 + EX31_RS19080 0.43 +0.1 409,425 + EX31_RS19080 0.43 +0.8 409,425 + EX31_RS19080 0.43 -1.2 409,425 + EX31_RS19080 0.43 -1.4 409,426 - EX31_RS19080 0.43 +1.4 409,426 - EX31_RS19080 0.43 +2.6 409,426 - EX31_RS19080 0.43 -1.3 409,426 - EX31_RS19080 0.43 -0.6 409,426 - EX31_RS19080 0.43 +0.2 409,426 - EX31_RS19080 0.43 +0.4 409,426 - EX31_RS19080 0.43 -1.7 409,426 - EX31_RS19080 0.43 -1.3 409,426 - EX31_RS19080 0.43 -2.2 409,426 - EX31_RS19080 0.43 -0.6 409,429 + EX31_RS19080 0.43 -0.3 409,429 + EX31_RS19080 0.43 -0.3 409,429 + EX31_RS19080 0.43 -0.6 409,429 + EX31_RS19080 0.43 -2.5 409,429 + EX31_RS19080 0.43 +0.7 409,429 + EX31_RS19080 0.43 -1.6 409,429 + EX31_RS19080 0.43 +0.2 409,429 + EX31_RS19080 0.43 -2.0 409,430 - EX31_RS19080 0.43 +0.6 409,430 - EX31_RS19080 0.43 +0.7 409,430 - EX31_RS19080 0.43 +1.6
Or see this region's nucleotide sequence