Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13470 and EX31_RS13475 are separated by 6 nucleotides EX31_RS13475 and EX31_RS13480 are separated by 25 nucleotides EX31_RS13480 and EX31_RS13485 are separated by 99 nucleotides EX31_RS13485 and EX31_RS13490 are separated by 45 nucleotides
EX31_RS13470: EX31_RS13470 - single-stranded-DNA-specific exonuclease RecJ, at 128,561 to 130,294
_RS13470
EX31_RS13475: EX31_RS13475 - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 130,301 to 131,020
_RS13475
EX31_RS13480: EX31_RS13480 - site-specific tyrosine recombinase XerD, at 131,046 to 132,005
_RS13480
EX31_RS13485: EX31_RS13485 - flavodoxin FldB, at 132,105 to 132,623
_RS13485
EX31_RS13490: EX31_RS13490 - membrane protein, at 132,669 to 133,121
_RS13490
Position (kb) 131
132
133 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 130.069 kb on + strand, within EX31_RS13470 at 130.070 kb on - strand, within EX31_RS13470 at 130.070 kb on - strand, within EX31_RS13470 at 130.070 kb on - strand, within EX31_RS13470 at 130.073 kb on + strand, within EX31_RS13470 at 130.074 kb on - strand, within EX31_RS13470 at 130.074 kb on - strand, within EX31_RS13470 at 130.144 kb on - strand at 130.144 kb on - strand at 130.153 kb on + strand at 130.154 kb on - strand at 130.207 kb on - strand at 130.207 kb on - strand at 130.315 kb on + strand at 130.315 kb on + strand at 130.315 kb on + strand at 130.315 kb on + strand at 130.316 kb on - strand at 130.316 kb on - strand at 130.374 kb on - strand, within EX31_RS13475 at 130.374 kb on - strand, within EX31_RS13475 at 130.457 kb on - strand, within EX31_RS13475 at 130.534 kb on + strand, within EX31_RS13475 at 130.534 kb on + strand, within EX31_RS13475 at 130.534 kb on + strand, within EX31_RS13475 at 130.535 kb on - strand, within EX31_RS13475 at 130.535 kb on - strand, within EX31_RS13475 at 130.535 kb on - strand, within EX31_RS13475 at 130.543 kb on + strand, within EX31_RS13475 at 130.543 kb on + strand, within EX31_RS13475 at 130.543 kb on + strand, within EX31_RS13475 at 130.543 kb on + strand, within EX31_RS13475 at 130.543 kb on + strand, within EX31_RS13475 at 130.544 kb on - strand, within EX31_RS13475 at 130.544 kb on - strand, within EX31_RS13475 at 130.544 kb on - strand, within EX31_RS13475 at 130.544 kb on - strand, within EX31_RS13475 at 130.544 kb on - strand, within EX31_RS13475 at 130.544 kb on - strand, within EX31_RS13475 at 130.544 kb on - strand, within EX31_RS13475 at 130.611 kb on - strand, within EX31_RS13475 at 130.624 kb on + strand, within EX31_RS13475 at 130.624 kb on + strand, within EX31_RS13475 at 130.625 kb on - strand, within EX31_RS13475 at 130.625 kb on - strand, within EX31_RS13475 at 130.626 kb on + strand, within EX31_RS13475 at 130.626 kb on + strand, within EX31_RS13475 at 130.626 kb on + strand, within EX31_RS13475 at 130.626 kb on + strand, within EX31_RS13475 at 130.626 kb on + strand, within EX31_RS13475 at 130.626 kb on + strand, within EX31_RS13475 at 130.626 kb on + strand, within EX31_RS13475 at 130.627 kb on - strand, within EX31_RS13475 at 130.627 kb on - strand, within EX31_RS13475 at 130.627 kb on - strand, within EX31_RS13475 at 130.627 kb on - strand, within EX31_RS13475 at 130.627 kb on - strand, within EX31_RS13475 at 130.627 kb on - strand, within EX31_RS13475 at 130.634 kb on + strand, within EX31_RS13475 at 130.634 kb on + strand, within EX31_RS13475 at 130.635 kb on - strand, within EX31_RS13475 at 130.660 kb on - strand, within EX31_RS13475 at 130.714 kb on + strand, within EX31_RS13475 at 130.714 kb on + strand, within EX31_RS13475 at 130.714 kb on + strand, within EX31_RS13475 at 130.716 kb on + strand, within EX31_RS13475 at 130.716 kb on + strand, within EX31_RS13475 at 130.716 kb on + strand, within EX31_RS13475 at 130.717 kb on - strand, within EX31_RS13475 at 130.717 kb on - strand, within EX31_RS13475 at 130.717 kb on - strand, within EX31_RS13475 at 130.737 kb on - strand, within EX31_RS13475 at 130.803 kb on + strand, within EX31_RS13475 at 130.804 kb on - strand, within EX31_RS13475 at 130.804 kb on - strand, within EX31_RS13475 at 130.804 kb on - strand, within EX31_RS13475 at 130.804 kb on - strand, within EX31_RS13475 at 130.804 kb on - strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.845 kb on + strand, within EX31_RS13475 at 130.846 kb on - strand, within EX31_RS13475 at 130.846 kb on - strand, within EX31_RS13475 at 130.846 kb on - strand, within EX31_RS13475 at 130.846 kb on - strand, within EX31_RS13475 at 130.846 kb on - strand, within EX31_RS13475 at 130.846 kb on - strand, within EX31_RS13475 at 130.846 kb on - strand, within EX31_RS13475 at 130.864 kb on + strand, within EX31_RS13475 at 130.864 kb on + strand, within EX31_RS13475 at 130.978 kb on + strand at 130.978 kb on + strand at 130.978 kb on + strand at 130.979 kb on - strand at 130.979 kb on - strand at 130.979 kb on - strand at 130.979 kb on - strand at 131.006 kb on + strand at 131.007 kb on - strand at 131.047 kb on + strand at 131.048 kb on - strand at 131.048 kb on - strand at 131.073 kb on + strand at 131.074 kb on - strand at 131.097 kb on + strand at 131.098 kb on - strand at 131.106 kb on - strand at 131.107 kb on + strand at 131.108 kb on - strand at 131.124 kb on + strand at 131.182 kb on - strand, within EX31_RS13480 at 131.254 kb on + strand, within EX31_RS13480 at 131.383 kb on + strand, within EX31_RS13480 at 131.430 kb on - strand, within EX31_RS13480 at 131.538 kb on - strand, within EX31_RS13480 at 131.590 kb on - strand, within EX31_RS13480 at 131.590 kb on - strand, within EX31_RS13480 at 131.718 kb on + strand, within EX31_RS13480 at 131.718 kb on + strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.757 kb on - strand, within EX31_RS13480 at 131.778 kb on + strand, within EX31_RS13480 at 131.816 kb on + strand, within EX31_RS13480 at 131.817 kb on - strand, within EX31_RS13480 at 131.817 kb on - strand, within EX31_RS13480 at 131.904 kb on - strand, within EX31_RS13480 at 132.018 kb on + strand at 132.019 kb on - strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.035 kb on - strand at 132.061 kb on - strand at 132.078 kb on + strand at 132.078 kb on + strand at 132.079 kb on - strand at 132.092 kb on + strand at 132.104 kb on + strand at 132.124 kb on - strand at 132.141 kb on + strand at 132.141 kb on + strand at 132.141 kb on + strand at 132.204 kb on - strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.240 kb on + strand, within EX31_RS13485 at 132.240 kb on + strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.334 kb on + strand, within EX31_RS13485 at 132.357 kb on + strand, within EX31_RS13485 at 132.417 kb on + strand, within EX31_RS13485 at 132.482 kb on + strand, within EX31_RS13485 at 132.631 kb on + strand at 132.632 kb on - strand at 132.632 kb on - strand at 132.632 kb on - strand at 132.690 kb on - strand at 132.711 kb on + strand at 132.711 kb on + strand at 132.711 kb on + strand at 132.809 kb on + strand, within EX31_RS13490 at 132.837 kb on + strand, within EX31_RS13490 at 132.838 kb on - strand, within EX31_RS13490 at 132.838 kb on - strand, within EX31_RS13490 at 132.838 kb on - strand, within EX31_RS13490 at 132.849 kb on + strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.858 kb on + strand, within EX31_RS13490 at 132.894 kb on + strand, within EX31_RS13490 at 132.894 kb on + strand, within EX31_RS13490 at 132.895 kb on - strand, within EX31_RS13490 at 132.942 kb on - strand, within EX31_RS13490 at 132.942 kb on - strand, within EX31_RS13490 at 132.944 kb on + strand, within EX31_RS13490 at 132.944 kb on + strand, within EX31_RS13490 at 132.945 kb on - strand, within EX31_RS13490 at 132.945 kb on - strand, within EX31_RS13490
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 130,069 + EX31_RS13470 0.87 +0.1 130,070 - EX31_RS13470 0.87 +0.4 130,070 - EX31_RS13470 0.87 +0.1 130,070 - EX31_RS13470 0.87 -0.0 130,073 + EX31_RS13470 0.87 +1.2 130,074 - EX31_RS13470 0.87 -0.4 130,074 - EX31_RS13470 0.87 -1.6 130,144 - -0.2 130,144 - +0.5 130,153 + +1.1 130,154 - +0.8 130,207 - -3.3 130,207 - -1.1 130,315 + +0.2 130,315 + -0.9 130,315 + +0.1 130,315 + -2.2 130,316 - +0.1 130,316 - -0.8 130,374 - EX31_RS13475 0.10 -0.0 130,374 - EX31_RS13475 0.10 +0.6 130,457 - EX31_RS13475 0.22 -0.2 130,534 + EX31_RS13475 0.32 -0.5 130,534 + EX31_RS13475 0.32 +1.2 130,534 + EX31_RS13475 0.32 -2.9 130,535 - EX31_RS13475 0.33 +0.7 130,535 - EX31_RS13475 0.33 +0.1 130,535 - EX31_RS13475 0.33 -1.6 130,543 + EX31_RS13475 0.34 -0.5 130,543 + EX31_RS13475 0.34 +0.8 130,543 + EX31_RS13475 0.34 +0.4 130,543 + EX31_RS13475 0.34 +0.9 130,543 + EX31_RS13475 0.34 -3.1 130,544 - EX31_RS13475 0.34 -0.1 130,544 - EX31_RS13475 0.34 -0.2 130,544 - EX31_RS13475 0.34 +2.4 130,544 - EX31_RS13475 0.34 -0.1 130,544 - EX31_RS13475 0.34 -0.3 130,544 - EX31_RS13475 0.34 +0.2 130,544 - EX31_RS13475 0.34 -1.4 130,611 - EX31_RS13475 0.43 -0.5 130,624 + EX31_RS13475 0.45 -2.5 130,624 + EX31_RS13475 0.45 +0.2 130,625 - EX31_RS13475 0.45 +0.6 130,625 - EX31_RS13475 0.45 -0.4 130,626 + EX31_RS13475 0.45 -1.1 130,626 + EX31_RS13475 0.45 -1.1 130,626 + EX31_RS13475 0.45 -2.5 130,626 + EX31_RS13475 0.45 -0.4 130,626 + EX31_RS13475 0.45 -1.6 130,626 + EX31_RS13475 0.45 -2.4 130,626 + EX31_RS13475 0.45 +0.5 130,627 - EX31_RS13475 0.45 +0.4 130,627 - EX31_RS13475 0.45 -0.2 130,627 - EX31_RS13475 0.45 +1.8 130,627 - EX31_RS13475 0.45 -1.9 130,627 - EX31_RS13475 0.45 -2.9 130,627 - EX31_RS13475 0.45 +1.0 130,634 + EX31_RS13475 0.46 +0.9 130,634 + EX31_RS13475 0.46 -0.9 130,635 - EX31_RS13475 0.46 -0.2 130,660 - EX31_RS13475 0.50 -0.2 130,714 + EX31_RS13475 0.57 -1.8 130,714 + EX31_RS13475 0.57 +0.0 130,714 + EX31_RS13475 0.57 +2.5 130,716 + EX31_RS13475 0.58 -2.9 130,716 + EX31_RS13475 0.58 -1.8 130,716 + EX31_RS13475 0.58 -2.7 130,717 - EX31_RS13475 0.58 -0.2 130,717 - EX31_RS13475 0.58 +0.5 130,717 - EX31_RS13475 0.58 +0.5 130,737 - EX31_RS13475 0.61 -0.6 130,803 + EX31_RS13475 0.70 -0.9 130,804 - EX31_RS13475 0.70 -1.9 130,804 - EX31_RS13475 0.70 +1.2 130,804 - EX31_RS13475 0.70 +0.6 130,804 - EX31_RS13475 0.70 -0.1 130,804 - EX31_RS13475 0.70 +1.0 130,845 + EX31_RS13475 0.76 +1.0 130,845 + EX31_RS13475 0.76 -1.4 130,845 + EX31_RS13475 0.76 -1.6 130,845 + EX31_RS13475 0.76 -0.3 130,845 + EX31_RS13475 0.76 -0.7 130,845 + EX31_RS13475 0.76 -0.8 130,845 + EX31_RS13475 0.76 +0.7 130,845 + EX31_RS13475 0.76 +0.1 130,845 + EX31_RS13475 0.76 +0.8 130,845 + EX31_RS13475 0.76 -0.6 130,846 - EX31_RS13475 0.76 +0.6 130,846 - EX31_RS13475 0.76 -0.0 130,846 - EX31_RS13475 0.76 -0.1 130,846 - EX31_RS13475 0.76 +1.9 130,846 - EX31_RS13475 0.76 +0.4 130,846 - EX31_RS13475 0.76 -0.2 130,846 - EX31_RS13475 0.76 +0.6 130,864 + EX31_RS13475 0.78 +1.3 130,864 + EX31_RS13475 0.78 -1.0 130,978 + -0.7 130,978 + +0.8 130,978 + -1.8 130,979 - -0.4 130,979 - -1.4 130,979 - +0.5 130,979 - -1.8 131,006 + -0.3 131,007 - -1.2 131,047 + +0.1 131,048 - -0.1 131,048 - -0.1 131,073 + +1.4 131,074 - +0.2 131,097 + -0.8 131,098 - +0.6 131,106 - -0.9 131,107 + +0.6 131,108 - +0.2 131,124 + +2.4 131,182 - EX31_RS13480 0.14 -2.4 131,254 + EX31_RS13480 0.22 -0.6 131,383 + EX31_RS13480 0.35 -2.7 131,430 - EX31_RS13480 0.40 -1.8 131,538 - EX31_RS13480 0.51 +0.1 131,590 - EX31_RS13480 0.57 -1.9 131,590 - EX31_RS13480 0.57 -1.0 131,718 + EX31_RS13480 0.70 +0.5 131,718 + EX31_RS13480 0.70 -2.2 131,719 - EX31_RS13480 0.70 -0.4 131,719 - EX31_RS13480 0.70 +0.4 131,719 - EX31_RS13480 0.70 -0.1 131,719 - EX31_RS13480 0.70 -1.9 131,719 - EX31_RS13480 0.70 +0.6 131,757 - EX31_RS13480 0.74 -1.9 131,778 + EX31_RS13480 0.76 -0.8 131,816 + EX31_RS13480 0.80 -2.4 131,817 - EX31_RS13480 0.80 -1.1 131,817 - EX31_RS13480 0.80 -1.8 131,904 - EX31_RS13480 0.89 +1.2 132,018 + -0.8 132,019 - +1.1 132,034 + -0.5 132,034 + +0.9 132,034 + +0.6 132,034 + +1.0 132,034 + +0.5 132,035 - +1.2 132,061 - +0.9 132,078 + -1.2 132,078 + -1.4 132,079 - +0.0 132,092 + +1.2 132,104 + +0.9 132,124 - -1.8 132,141 + +0.0 132,141 + +0.5 132,141 + -1.4 132,204 - EX31_RS13485 0.19 +2.0 132,208 + EX31_RS13485 0.20 +0.5 132,208 + EX31_RS13485 0.20 -0.6 132,208 + EX31_RS13485 0.20 +0.5 132,208 + EX31_RS13485 0.20 -0.4 132,209 - EX31_RS13485 0.20 +0.2 132,209 - EX31_RS13485 0.20 +0.2 132,209 - EX31_RS13485 0.20 +0.0 132,209 - EX31_RS13485 0.20 -0.9 132,209 - EX31_RS13485 0.20 +1.2 132,209 - EX31_RS13485 0.20 -1.8 132,209 - EX31_RS13485 0.20 +1.2 132,209 - EX31_RS13485 0.20 -1.4 132,209 - EX31_RS13485 0.20 +1.1 132,209 - EX31_RS13485 0.20 -2.2 132,240 + EX31_RS13485 0.26 -0.4 132,240 + EX31_RS13485 0.26 +3.2 132,241 - EX31_RS13485 0.26 -2.5 132,241 - EX31_RS13485 0.26 +0.5 132,241 - EX31_RS13485 0.26 -3.2 132,241 - EX31_RS13485 0.26 +0.2 132,241 - EX31_RS13485 0.26 -1.4 132,334 + EX31_RS13485 0.44 -2.5 132,357 + EX31_RS13485 0.49 +0.2 132,417 + EX31_RS13485 0.60 +0.0 132,482 + EX31_RS13485 0.73 +2.6 132,631 + -2.7 132,632 - +0.3 132,632 - +0.1 132,632 - -0.8 132,690 - +1.2 132,711 + -0.0 132,711 + +0.2 132,711 + -2.5 132,809 + EX31_RS13490 0.31 +0.4 132,837 + EX31_RS13490 0.37 -0.4 132,838 - EX31_RS13490 0.37 -0.0 132,838 - EX31_RS13490 0.37 -1.5 132,838 - EX31_RS13490 0.37 -1.5 132,849 + EX31_RS13490 0.40 -1.0 132,850 - EX31_RS13490 0.40 +0.2 132,850 - EX31_RS13490 0.40 -0.5 132,850 - EX31_RS13490 0.40 +0.8 132,850 - EX31_RS13490 0.40 -1.9 132,850 - EX31_RS13490 0.40 -1.0 132,850 - EX31_RS13490 0.40 -0.2 132,850 - EX31_RS13490 0.40 -1.1 132,850 - EX31_RS13490 0.40 +0.2 132,858 + EX31_RS13490 0.42 -1.4 132,894 + EX31_RS13490 0.50 +0.6 132,894 + EX31_RS13490 0.50 -0.4 132,895 - EX31_RS13490 0.50 +0.5 132,942 - EX31_RS13490 0.60 +0.5 132,942 - EX31_RS13490 0.60 -1.6 132,944 + EX31_RS13490 0.61 +0.8 132,944 + EX31_RS13490 0.61 -0.4 132,945 - EX31_RS13490 0.61 -2.5 132,945 - EX31_RS13490 0.61 +1.2
Or see this region's nucleotide sequence