Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11550 and EX31_RS11555 are separated by 152 nucleotides EX31_RS11555 and EX31_RS11560 are separated by 144 nucleotides EX31_RS11560 and EX31_RS11565 are separated by 168 nucleotides
EX31_RS11550: EX31_RS11550 - iron-sulfur cluster carrier protein ApbC, at 248,519 to 249,631
_RS11550
EX31_RS11555: EX31_RS11555 - methionine--tRNA ligase, at 249,784 to 251,814
_RS11555
EX31_RS11560: EX31_RS11560 - DUF1127 domain-containing protein, at 251,959 to 252,231
_RS11560
EX31_RS11565: EX31_RS11565 - PLP-dependent aminotransferase family protein, at 252,400 to 253,818
_RS11565
Position (kb) 249
250
251
252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 248.791 kb on + strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.804 kb on + strand, within EX31_RS11550 at 248.897 kb on + strand, within EX31_RS11550 at 248.942 kb on - strand, within EX31_RS11550 at 248.976 kb on + strand, within EX31_RS11550 at 248.999 kb on - strand, within EX31_RS11550 at 249.169 kb on + strand, within EX31_RS11550 at 249.169 kb on + strand, within EX31_RS11550 at 249.169 kb on + strand, within EX31_RS11550 at 249.190 kb on - strand, within EX31_RS11550 at 249.304 kb on + strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.432 kb on - strand, within EX31_RS11550 at 249.435 kb on - strand, within EX31_RS11550 at 249.478 kb on + strand, within EX31_RS11550 at 249.478 kb on + strand, within EX31_RS11550 at 249.479 kb on - strand, within EX31_RS11550 at 249.479 kb on - strand, within EX31_RS11550 at 249.479 kb on - strand, within EX31_RS11550 at 249.479 kb on - strand, within EX31_RS11550 at 249.482 kb on + strand, within EX31_RS11550 at 249.482 kb on + strand, within EX31_RS11550 at 249.482 kb on + strand, within EX31_RS11550 at 249.482 kb on + strand, within EX31_RS11550 at 249.482 kb on + strand, within EX31_RS11550 at 249.483 kb on - strand, within EX31_RS11550 at 249.483 kb on - strand, within EX31_RS11550 at 249.483 kb on - strand, within EX31_RS11550 at 249.497 kb on + strand, within EX31_RS11550 at 249.497 kb on + strand, within EX31_RS11550 at 249.569 kb on + strand at 249.661 kb on + strand at 249.661 kb on + strand at 249.662 kb on - strand at 249.662 kb on - strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.665 kb on + strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.666 kb on - strand at 249.669 kb on + strand at 249.670 kb on - strand at 249.670 kb on - strand at 249.675 kb on + strand at 249.675 kb on + strand at 249.676 kb on - strand at 249.676 kb on - strand at 249.676 kb on - strand at 249.682 kb on - strand at 249.695 kb on + strand at 249.695 kb on + strand at 249.696 kb on - strand at 249.696 kb on - strand at 251.447 kb on + strand, within EX31_RS11555 at 251.915 kb on - strand at 251.956 kb on + strand at 252.093 kb on + strand, within EX31_RS11560 at 252.093 kb on + strand, within EX31_RS11560 at 252.093 kb on + strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.171 kb on + strand, within EX31_RS11560 at 252.223 kb on - strand at 252.223 kb on - strand at 252.268 kb on - strand at 252.441 kb on + strand at 252.458 kb on - strand at 252.494 kb on + strand at 252.660 kb on + strand, within EX31_RS11565 at 252.661 kb on - strand, within EX31_RS11565 at 252.693 kb on + strand, within EX31_RS11565 at 252.693 kb on + strand, within EX31_RS11565 at 252.694 kb on - strand, within EX31_RS11565
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 248,791 + EX31_RS11550 0.24 -1.5 248,792 - EX31_RS11550 0.25 -0.1 248,792 - EX31_RS11550 0.25 -0.5 248,792 - EX31_RS11550 0.25 +1.2 248,792 - EX31_RS11550 0.25 +0.1 248,792 - EX31_RS11550 0.25 +0.1 248,804 + EX31_RS11550 0.26 +0.9 248,897 + EX31_RS11550 0.34 -1.1 248,942 - EX31_RS11550 0.38 +1.1 248,976 + EX31_RS11550 0.41 -1.3 248,999 - EX31_RS11550 0.43 -2.1 249,169 + EX31_RS11550 0.58 +0.4 249,169 + EX31_RS11550 0.58 -1.5 249,169 + EX31_RS11550 0.58 -0.3 249,190 - EX31_RS11550 0.60 +0.2 249,304 + EX31_RS11550 0.71 +0.6 249,432 - EX31_RS11550 0.82 -0.7 249,432 - EX31_RS11550 0.82 -1.3 249,432 - EX31_RS11550 0.82 -3.3 249,432 - EX31_RS11550 0.82 +0.3 249,432 - EX31_RS11550 0.82 -1.5 249,432 - EX31_RS11550 0.82 +0.3 249,432 - EX31_RS11550 0.82 -2.7 249,432 - EX31_RS11550 0.82 +1.9 249,432 - EX31_RS11550 0.82 +0.3 249,435 - EX31_RS11550 0.82 -1.9 249,478 + EX31_RS11550 0.86 -0.7 249,478 + EX31_RS11550 0.86 -0.7 249,479 - EX31_RS11550 0.86 -0.4 249,479 - EX31_RS11550 0.86 +1.9 249,479 - EX31_RS11550 0.86 -0.1 249,479 - EX31_RS11550 0.86 +1.2 249,482 + EX31_RS11550 0.87 +0.9 249,482 + EX31_RS11550 0.87 -1.7 249,482 + EX31_RS11550 0.87 +0.9 249,482 + EX31_RS11550 0.87 -2.6 249,482 + EX31_RS11550 0.87 -0.1 249,483 - EX31_RS11550 0.87 +3.4 249,483 - EX31_RS11550 0.87 +1.8 249,483 - EX31_RS11550 0.87 -0.6 249,497 + EX31_RS11550 0.88 +2.9 249,497 + EX31_RS11550 0.88 -0.5 249,569 + +0.7 249,661 + +0.9 249,661 + -1.5 249,662 - -0.1 249,662 - -0.7 249,665 + +1.1 249,665 + +0.0 249,665 + -2.1 249,665 + +0.9 249,665 + -0.7 249,665 + +1.1 249,665 + -2.3 249,665 + -1.5 249,665 + +1.4 249,665 + +0.8 249,666 - +1.9 249,666 - +1.0 249,666 - +0.4 249,666 - +1.2 249,666 - +2.4 249,666 - +1.9 249,666 - +1.7 249,666 - -0.7 249,666 - +1.6 249,666 - -1.1 249,666 - -0.3 249,666 - +0.9 249,666 - +0.8 249,666 - -2.7 249,669 + -0.1 249,670 - -0.9 249,670 - +0.9 249,675 + +0.7 249,675 + +0.9 249,676 - -0.1 249,676 - +0.3 249,676 - +0.4 249,682 - +0.9 249,695 + +1.1 249,695 + +3.7 249,696 - +0.4 249,696 - -1.1 251,447 + EX31_RS11555 0.82 -2.7 251,915 - +0.2 251,956 + +0.6 252,093 + EX31_RS11560 0.49 +1.9 252,093 + EX31_RS11560 0.49 -1.1 252,093 + EX31_RS11560 0.49 +0.1 252,094 - EX31_RS11560 0.49 +1.0 252,094 - EX31_RS11560 0.49 -0.9 252,094 - EX31_RS11560 0.49 -1.9 252,094 - EX31_RS11560 0.49 -0.6 252,094 - EX31_RS11560 0.49 -1.1 252,094 - EX31_RS11560 0.49 -1.5 252,171 + EX31_RS11560 0.78 +1.9 252,223 - +1.3 252,223 - +0.9 252,268 - -1.9 252,441 + -0.5 252,458 - +0.9 252,494 + -0.1 252,660 + EX31_RS11565 0.18 +0.4 252,661 - EX31_RS11565 0.18 +1.4 252,693 + EX31_RS11565 0.21 +0.1 252,693 + EX31_RS11565 0.21 -0.1 252,694 - EX31_RS11565 0.21 -1.5
Or see this region's nucleotide sequence