Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07410 and EX31_RS07415 are separated by 303 nucleotides EX31_RS07415 and EX31_RS07420 overlap by 55 nucleotides
EX31_RS07410: EX31_RS07410 - NAD(P)H-dependent oxidoreductase, at 295,234 to 295,872
_RS07410
EX31_RS07415: EX31_RS07415 - bifunctional diguanylate cyclase/phosphodiesterase, at 296,176 to 297,726
_RS07415
EX31_RS07420: EX31_RS07420 - bifunctional helix-turn-helix transcriptional regulator/GNAT family N-acetyltransferase, at 297,672 to 298,673
_RS07420
Position (kb) 296
297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 295.182 kb on + strand at 295.183 kb on - strand at 295.245 kb on - strand at 295.323 kb on + strand, within EX31_RS07410 at 295.323 kb on + strand, within EX31_RS07410 at 295.618 kb on + strand, within EX31_RS07410 at 295.642 kb on + strand, within EX31_RS07410 at 295.643 kb on - strand, within EX31_RS07410 at 295.643 kb on - strand, within EX31_RS07410 at 295.870 kb on + strand at 295.870 kb on + strand at 295.928 kb on - strand at 295.953 kb on - strand at 295.960 kb on + strand at 295.960 kb on + strand at 295.960 kb on + strand at 295.961 kb on - strand at 295.982 kb on + strand at 295.982 kb on + strand at 295.983 kb on - strand at 295.983 kb on - strand at 295.984 kb on + strand at 295.984 kb on + strand at 295.984 kb on + strand at 295.985 kb on - strand at 295.985 kb on - strand at 296.058 kb on + strand at 296.058 kb on + strand at 296.059 kb on - strand at 296.059 kb on - strand at 296.059 kb on - strand at 296.059 kb on - strand at 296.059 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.215 kb on + strand at 296.799 kb on + strand, within EX31_RS07415 at 297.356 kb on - strand, within EX31_RS07415 at 297.498 kb on + strand, within EX31_RS07415 at 297.586 kb on - strand at 297.586 kb on - strand at 298.132 kb on + strand, within EX31_RS07420 at 298.137 kb on - strand, within EX31_RS07420 at 298.144 kb on + strand, within EX31_RS07420 at 298.145 kb on - strand, within EX31_RS07420 at 298.147 kb on + strand, within EX31_RS07420 at 298.148 kb on - strand, within EX31_RS07420 at 298.148 kb on - strand, within EX31_RS07420 at 298.148 kb on - strand, within EX31_RS07420 at 298.148 kb on - strand, within EX31_RS07420 at 298.268 kb on - strand, within EX31_RS07420 at 298.268 kb on - strand, within EX31_RS07420 at 298.305 kb on - strand, within EX31_RS07420 at 298.305 kb on - strand, within EX31_RS07420 at 298.305 kb on - strand, within EX31_RS07420 at 298.305 kb on - strand, within EX31_RS07420 at 298.305 kb on - strand, within EX31_RS07420 at 298.349 kb on - strand, within EX31_RS07420 at 298.576 kb on + strand at 298.605 kb on - strand at 298.626 kb on - strand at 298.679 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 295,182 + +0.6 295,183 - +0.7 295,245 - -0.9 295,323 + EX31_RS07410 0.14 +1.0 295,323 + EX31_RS07410 0.14 +3.0 295,618 + EX31_RS07410 0.60 +0.3 295,642 + EX31_RS07410 0.64 -0.0 295,643 - EX31_RS07410 0.64 +1.1 295,643 - EX31_RS07410 0.64 -0.6 295,870 + -0.1 295,870 + +3.3 295,928 - +0.7 295,953 - -0.6 295,960 + -1.1 295,960 + -0.5 295,960 + -1.6 295,961 - +3.8 295,982 + -0.3 295,982 + +1.7 295,983 - +0.7 295,983 - +3.6 295,984 + +1.5 295,984 + -1.3 295,984 + +1.4 295,985 - -2.1 295,985 - +1.5 296,058 + +0.7 296,058 + -0.3 296,059 - +0.7 296,059 - -0.3 296,059 - +2.2 296,059 - +0.7 296,059 - -2.8 296,176 - -0.3 296,176 - +1.9 296,176 - +2.7 296,215 + +0.4 296,799 + EX31_RS07415 0.40 -0.9 297,356 - EX31_RS07415 0.76 -0.5 297,498 + EX31_RS07415 0.85 -2.3 297,586 - -2.5 297,586 - -1.6 298,132 + EX31_RS07420 0.46 -2.3 298,137 - EX31_RS07420 0.46 +0.8 298,144 + EX31_RS07420 0.47 -0.3 298,145 - EX31_RS07420 0.47 +0.9 298,147 + EX31_RS07420 0.47 +1.0 298,148 - EX31_RS07420 0.48 -0.6 298,148 - EX31_RS07420 0.48 +0.9 298,148 - EX31_RS07420 0.48 +1.4 298,148 - EX31_RS07420 0.48 +1.9 298,268 - EX31_RS07420 0.59 +1.2 298,268 - EX31_RS07420 0.59 -0.5 298,305 - EX31_RS07420 0.63 -0.2 298,305 - EX31_RS07420 0.63 +0.7 298,305 - EX31_RS07420 0.63 +0.4 298,305 - EX31_RS07420 0.63 -0.2 298,305 - EX31_RS07420 0.63 +0.0 298,349 - EX31_RS07420 0.68 -0.0 298,576 + +0.7 298,605 - +2.1 298,626 - -0.7 298,679 + -0.3
Or see this region's nucleotide sequence