Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03030 and EX31_RS03035 overlap by 14 nucleotides EX31_RS03035 and EX31_RS03040 overlap by 4 nucleotides EX31_RS03040 and EX31_RS03045 are separated by 155 nucleotides EX31_RS03045 and EX31_RS03050 are separated by 154 nucleotides
EX31_RS03030: EX31_RS03030 - helix-turn-helix transcriptional regulator, at 88,178 to 88,984
_RS03030
EX31_RS03035: EX31_RS03035 - LysE family transporter, at 88,971 to 89,609
_RS03035
EX31_RS03040: EX31_RS03040 - hypothetical protein, at 89,606 to 90,898
_RS03040
EX31_RS03045: EX31_RS03045 - DUF1345 domain-containing protein, at 91,054 to 91,713
_RS03045
EX31_RS03050: EX31_RS03050 - transporter substrate-binding domain-containing protein, at 91,868 to 92,686
_RS03050
Position (kb) 89
90
91 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 88.676 kb on - strand, within EX31_RS03030 at 88.676 kb on - strand, within EX31_RS03030 at 88.793 kb on + strand, within EX31_RS03030 at 88.793 kb on + strand, within EX31_RS03030 at 88.794 kb on - strand, within EX31_RS03030 at 88.794 kb on - strand, within EX31_RS03030 at 88.798 kb on + strand, within EX31_RS03030 at 88.798 kb on + strand, within EX31_RS03030 at 88.798 kb on + strand, within EX31_RS03030 at 88.799 kb on - strand, within EX31_RS03030 at 88.799 kb on - strand, within EX31_RS03030 at 88.799 kb on - strand, within EX31_RS03030 at 88.846 kb on + strand, within EX31_RS03030 at 88.846 kb on + strand, within EX31_RS03030 at 88.847 kb on - strand, within EX31_RS03030 at 88.847 kb on - strand, within EX31_RS03030 at 88.867 kb on - strand, within EX31_RS03030 at 88.881 kb on - strand, within EX31_RS03030 at 88.881 kb on - strand, within EX31_RS03030 at 88.881 kb on - strand, within EX31_RS03030 at 88.903 kb on + strand, within EX31_RS03030 at 88.904 kb on - strand at 88.904 kb on - strand at 88.904 kb on - strand at 88.904 kb on - strand at 88.912 kb on + strand at 88.940 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.973 kb on - strand at 88.973 kb on - strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.975 kb on - strand at 88.975 kb on - strand at 88.975 kb on - strand at 88.975 kb on - strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.023 kb on + strand at 89.023 kb on + strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.050 kb on + strand, within EX31_RS03035 at 89.050 kb on + strand, within EX31_RS03035 at 89.050 kb on + strand, within EX31_RS03035 at 89.050 kb on + strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.056 kb on + strand, within EX31_RS03035 at 89.056 kb on + strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.360 kb on + strand, within EX31_RS03035 at 89.360 kb on + strand, within EX31_RS03035 at 89.361 kb on - strand, within EX31_RS03035 at 89.361 kb on - strand, within EX31_RS03035 at 89.416 kb on + strand, within EX31_RS03035 at 89.417 kb on - strand, within EX31_RS03035 at 89.417 kb on - strand, within EX31_RS03035 at 89.459 kb on - strand, within EX31_RS03035 at 89.463 kb on + strand, within EX31_RS03035 at 89.463 kb on + strand, within EX31_RS03035 at 89.464 kb on - strand, within EX31_RS03035 at 89.464 kb on - strand, within EX31_RS03035 at 89.546 kb on + strand at 89.547 kb on - strand at 89.584 kb on - strand at 89.609 kb on + strand at 89.623 kb on + strand at 89.624 kb on - strand at 89.624 kb on - strand at 89.628 kb on - strand at 89.628 kb on - strand at 89.637 kb on + strand at 89.637 kb on + strand at 89.637 kb on + strand at 89.638 kb on - strand at 89.638 kb on - strand at 89.638 kb on - strand at 89.659 kb on + strand at 89.659 kb on + strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.946 kb on + strand, within EX31_RS03040 at 89.946 kb on + strand, within EX31_RS03040 at 89.946 kb on + strand, within EX31_RS03040 at 89.947 kb on - strand, within EX31_RS03040 at 89.947 kb on - strand, within EX31_RS03040 at 90.014 kb on + strand, within EX31_RS03040 at 90.014 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.048 kb on - strand, within EX31_RS03040 at 90.150 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.247 kb on + strand, within EX31_RS03040 at 90.248 kb on - strand, within EX31_RS03040 at 90.248 kb on - strand, within EX31_RS03040 at 90.312 kb on + strand, within EX31_RS03040 at 90.312 kb on + strand, within EX31_RS03040 at 90.330 kb on + strand, within EX31_RS03040 at 90.334 kb on + strand, within EX31_RS03040 at 90.335 kb on - strand, within EX31_RS03040 at 90.375 kb on + strand, within EX31_RS03040 at 90.375 kb on + strand, within EX31_RS03040 at 90.427 kb on - strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.432 kb on - strand, within EX31_RS03040 at 90.432 kb on - strand, within EX31_RS03040 at 90.432 kb on - strand, within EX31_RS03040 at 90.510 kb on + strand, within EX31_RS03040 at 90.550 kb on + strand, within EX31_RS03040 at 90.602 kb on + strand, within EX31_RS03040 at 90.602 kb on + strand, within EX31_RS03040 at 90.603 kb on - strand, within EX31_RS03040 at 90.793 kb on - strand at 90.821 kb on + strand at 90.821 kb on + strand at 90.861 kb on + strand at 90.862 kb on - strand at 90.862 kb on - strand at 90.892 kb on + strand at 90.892 kb on + strand at 90.893 kb on - strand at 90.893 kb on - strand at 90.893 kb on - strand at 90.898 kb on + strand at 90.899 kb on - strand at 90.932 kb on + strand at 90.932 kb on + strand at 90.933 kb on - strand at 90.936 kb on + strand at 90.936 kb on + strand at 90.937 kb on - strand at 90.937 kb on - strand at 90.937 kb on - strand at 90.977 kb on + strand at 90.977 kb on + strand at 90.978 kb on - strand at 90.978 kb on - strand at 91.011 kb on - strand at 91.016 kb on + strand at 91.016 kb on + strand at 91.016 kb on + strand at 91.017 kb on - strand at 91.017 kb on - strand at 91.017 kb on - strand at 91.017 kb on - strand at 91.017 kb on - strand at 91.017 kb on - strand at 91.017 kb on - strand at 91.017 kb on - strand at 91.021 kb on + strand at 91.021 kb on + strand at 91.021 kb on + strand at 91.036 kb on + strand at 91.037 kb on - strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.075 kb on + strand at 91.076 kb on - strand at 91.076 kb on - strand at 91.107 kb on + strand at 91.108 kb on - strand at 91.108 kb on - strand at 91.108 kb on - strand at 91.108 kb on - strand at 91.263 kb on + strand, within EX31_RS03045 at 91.360 kb on - strand, within EX31_RS03045 at 91.392 kb on + strand, within EX31_RS03045 at 91.392 kb on + strand, within EX31_RS03045 at 91.392 kb on + strand, within EX31_RS03045 at 91.392 kb on + strand, within EX31_RS03045 at 91.392 kb on + strand, within EX31_RS03045 at 91.393 kb on - strand, within EX31_RS03045 at 91.393 kb on - strand, within EX31_RS03045 at 91.393 kb on - strand, within EX31_RS03045 at 91.393 kb on - strand, within EX31_RS03045 at 91.393 kb on - strand, within EX31_RS03045 at 91.410 kb on + strand, within EX31_RS03045 at 91.459 kb on + strand, within EX31_RS03045 at 91.460 kb on - strand, within EX31_RS03045 at 91.474 kb on + strand, within EX31_RS03045 at 91.475 kb on - strand, within EX31_RS03045 at 91.475 kb on - strand, within EX31_RS03045 at 91.614 kb on + strand, within EX31_RS03045 at 91.660 kb on - strand at 91.673 kb on + strand at 91.704 kb on - strand at 91.712 kb on - strand at 91.727 kb on + strand at 91.727 kb on + strand at 91.727 kb on + strand at 91.727 kb on + strand at 91.727 kb on + strand at 91.727 kb on + strand at 91.728 kb on - strand at 91.728 kb on - strand at 91.728 kb on - strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.769 kb on - strand at 91.769 kb on - strand at 91.769 kb on - strand at 91.799 kb on + strand at 91.799 kb on + strand at 91.800 kb on - strand at 91.800 kb on - strand at 91.800 kb on - strand at 91.817 kb on + strand at 91.817 kb on + strand at 91.817 kb on + strand at 91.817 kb on + strand at 91.817 kb on + strand at 91.818 kb on - strand at 91.818 kb on - strand at 91.818 kb on - strand at 91.830 kb on + strand at 91.847 kb on + strand at 91.854 kb on - strand at 91.868 kb on - strand at 91.884 kb on + strand at 91.884 kb on + strand at 91.884 kb on + strand at 91.885 kb on - strand at 91.885 kb on - strand at 91.885 kb on - strand at 91.885 kb on - strand at 91.885 kb on - strand at 91.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 88,676 - EX31_RS03030 0.62 -1.3 88,676 - EX31_RS03030 0.62 -2.2 88,793 + EX31_RS03030 0.76 -4.3 88,793 + EX31_RS03030 0.76 -1.2 88,794 - EX31_RS03030 0.76 -2.0 88,794 - EX31_RS03030 0.76 -1.2 88,798 + EX31_RS03030 0.77 -2.3 88,798 + EX31_RS03030 0.77 -3.9 88,798 + EX31_RS03030 0.77 -3.3 88,799 - EX31_RS03030 0.77 -4.5 88,799 - EX31_RS03030 0.77 -3.2 88,799 - EX31_RS03030 0.77 -0.4 88,846 + EX31_RS03030 0.83 -1.9 88,846 + EX31_RS03030 0.83 -1.2 88,847 - EX31_RS03030 0.83 -0.4 88,847 - EX31_RS03030 0.83 -2.8 88,867 - EX31_RS03030 0.85 -2.9 88,881 - EX31_RS03030 0.87 -1.2 88,881 - EX31_RS03030 0.87 +0.2 88,881 - EX31_RS03030 0.87 -1.2 88,903 + EX31_RS03030 0.90 -2.9 88,904 - +1.2 88,904 - -2.7 88,904 - -1.8 88,904 - +1.2 88,912 + +1.2 88,940 + -2.8 88,972 + -0.4 88,972 + -2.2 88,972 + -0.4 88,972 + -0.5 88,972 + +0.6 88,972 + +2.2 88,972 + +1.4 88,972 + +0.7 88,972 + +1.6 88,972 + -2.7 88,973 - +0.1 88,973 - -0.3 88,974 + -2.4 88,974 + -0.4 88,974 + -2.9 88,974 + -2.3 88,974 + -2.7 88,975 - -0.4 88,975 - -1.8 88,975 - -0.4 88,975 - -1.2 89,011 + -1.8 89,011 + -3.8 89,011 + -1.2 89,011 + -0.8 89,011 + -2.7 89,012 - -2.1 89,012 - -3.2 89,012 - +0.2 89,012 - -2.8 89,012 - -1.4 89,012 - -3.0 89,012 - -1.0 89,023 + -1.2 89,023 + -2.3 89,024 - -1.4 89,024 - -2.6 89,024 - -2.0 89,024 - +1.2 89,024 - -3.4 89,024 - -3.9 89,050 + EX31_RS03035 0.12 -2.7 89,050 + EX31_RS03035 0.12 -3.7 89,050 + EX31_RS03035 0.12 -3.5 89,050 + EX31_RS03035 0.12 -3.2 89,051 - EX31_RS03035 0.13 -2.2 89,051 - EX31_RS03035 0.13 -1.8 89,051 - EX31_RS03035 0.13 -3.6 89,051 - EX31_RS03035 0.13 -1.2 89,051 - EX31_RS03035 0.13 -1.4 89,051 - EX31_RS03035 0.13 -1.6 89,051 - EX31_RS03035 0.13 -2.8 89,051 - EX31_RS03035 0.13 -3.2 89,051 - EX31_RS03035 0.13 +0.3 89,051 - EX31_RS03035 0.13 -2.2 89,051 - EX31_RS03035 0.13 -2.7 89,056 + EX31_RS03035 0.13 -1.2 89,056 + EX31_RS03035 0.13 -2.6 89,057 - EX31_RS03035 0.13 -2.3 89,057 - EX31_RS03035 0.13 -3.9 89,057 - EX31_RS03035 0.13 -0.8 89,057 - EX31_RS03035 0.13 -1.2 89,360 + EX31_RS03035 0.61 -2.4 89,360 + EX31_RS03035 0.61 -1.2 89,361 - EX31_RS03035 0.61 -3.3 89,361 - EX31_RS03035 0.61 -2.0 89,416 + EX31_RS03035 0.70 -0.8 89,417 - EX31_RS03035 0.70 -1.2 89,417 - EX31_RS03035 0.70 -0.8 89,459 - EX31_RS03035 0.76 -4.2 89,463 + EX31_RS03035 0.77 +1.2 89,463 + EX31_RS03035 0.77 -0.9 89,464 - EX31_RS03035 0.77 -3.4 89,464 - EX31_RS03035 0.77 -3.0 89,546 + +0.2 89,547 - -2.5 89,584 - -2.7 89,609 + -2.5 89,623 + -2.4 89,624 - -3.2 89,624 - -2.9 89,628 - -1.2 89,628 - -3.9 89,637 + -3.2 89,637 + -3.5 89,637 + -4.0 89,638 - -3.1 89,638 - -1.4 89,638 - -1.6 89,659 + -2.0 89,659 + -1.8 89,660 - -0.4 89,660 - +0.7 89,866 - EX31_RS03040 0.20 -0.3 89,866 - EX31_RS03040 0.20 -1.5 89,866 - EX31_RS03040 0.20 -3.4 89,866 - EX31_RS03040 0.20 -2.8 89,866 - EX31_RS03040 0.20 -0.2 89,946 + EX31_RS03040 0.26 -1.2 89,946 + EX31_RS03040 0.26 -4.1 89,946 + EX31_RS03040 0.26 -0.4 89,947 - EX31_RS03040 0.26 -0.4 89,947 - EX31_RS03040 0.26 -1.8 90,014 + EX31_RS03040 0.32 -0.8 90,014 + EX31_RS03040 0.32 -0.4 90,047 + EX31_RS03040 0.34 -3.8 90,047 + EX31_RS03040 0.34 -3.5 90,047 + EX31_RS03040 0.34 -2.2 90,047 + EX31_RS03040 0.34 -2.4 90,047 + EX31_RS03040 0.34 -3.6 90,047 + EX31_RS03040 0.34 -0.8 90,048 - EX31_RS03040 0.34 -3.5 90,150 + EX31_RS03040 0.42 -1.4 90,243 + EX31_RS03040 0.49 +0.5 90,243 + EX31_RS03040 0.49 -2.5 90,243 + EX31_RS03040 0.49 -2.6 90,243 + EX31_RS03040 0.49 -0.5 90,244 - EX31_RS03040 0.49 -1.8 90,244 - EX31_RS03040 0.49 -4.5 90,244 - EX31_RS03040 0.49 +2.0 90,244 - EX31_RS03040 0.49 -2.9 90,247 + EX31_RS03040 0.50 -3.6 90,248 - EX31_RS03040 0.50 -1.0 90,248 - EX31_RS03040 0.50 -0.6 90,312 + EX31_RS03040 0.55 -1.2 90,312 + EX31_RS03040 0.55 -1.6 90,330 + EX31_RS03040 0.56 -1.6 90,334 + EX31_RS03040 0.56 -4.0 90,335 - EX31_RS03040 0.56 -0.6 90,375 + EX31_RS03040 0.59 -1.2 90,375 + EX31_RS03040 0.59 -2.8 90,427 - EX31_RS03040 0.63 -2.5 90,431 + EX31_RS03040 0.64 -1.3 90,431 + EX31_RS03040 0.64 -1.2 90,431 + EX31_RS03040 0.64 -3.0 90,431 + EX31_RS03040 0.64 -2.7 90,432 - EX31_RS03040 0.64 -1.4 90,432 - EX31_RS03040 0.64 -1.9 90,432 - EX31_RS03040 0.64 -2.7 90,510 + EX31_RS03040 0.70 -3.0 90,550 + EX31_RS03040 0.73 -2.6 90,602 + EX31_RS03040 0.77 -2.0 90,602 + EX31_RS03040 0.77 +0.2 90,603 - EX31_RS03040 0.77 -0.8 90,793 - -0.8 90,821 + -2.2 90,821 + -2.1 90,861 + -0.4 90,862 - -1.6 90,862 - -1.9 90,892 + -2.3 90,892 + -2.7 90,893 - -1.2 90,893 - -1.8 90,893 - -2.2 90,898 + -2.6 90,899 - -2.9 90,932 + -2.1 90,932 + +0.2 90,933 - -4.2 90,936 + -0.8 90,936 + -3.3 90,937 - -2.3 90,937 - -1.4 90,937 - -1.8 90,977 + -2.0 90,977 + +0.7 90,978 - -1.9 90,978 - -1.8 91,011 - -0.3 91,016 + +1.0 91,016 + -2.8 91,016 + -1.2 91,017 - -0.2 91,017 - +0.6 91,017 - -0.1 91,017 - +0.0 91,017 - +2.0 91,017 - -2.2 91,017 - -1.8 91,017 - -2.2 91,021 + +0.3 91,021 + +0.9 91,021 + -2.0 91,036 + -0.2 91,037 - +2.2 91,075 + +0.6 91,075 + +1.5 91,075 + +0.5 91,076 - +0.8 91,076 - -2.7 91,107 + -0.5 91,108 - +0.7 91,108 - -0.8 91,108 - -0.1 91,108 - +0.1 91,263 + EX31_RS03045 0.32 -0.0 91,360 - EX31_RS03045 0.46 +0.8 91,392 + EX31_RS03045 0.51 -0.1 91,392 + EX31_RS03045 0.51 -0.1 91,392 + EX31_RS03045 0.51 -2.5 91,392 + EX31_RS03045 0.51 +0.9 91,392 + EX31_RS03045 0.51 -1.2 91,393 - EX31_RS03045 0.51 +0.9 91,393 - EX31_RS03045 0.51 +0.7 91,393 - EX31_RS03045 0.51 +0.9 91,393 - EX31_RS03045 0.51 +0.5 91,393 - EX31_RS03045 0.51 -0.6 91,410 + EX31_RS03045 0.54 -0.0 91,459 + EX31_RS03045 0.61 -0.4 91,460 - EX31_RS03045 0.62 -0.7 91,474 + EX31_RS03045 0.64 +0.4 91,475 - EX31_RS03045 0.64 +1.8 91,475 - EX31_RS03045 0.64 +1.4 91,614 + EX31_RS03045 0.85 -0.7 91,660 - +0.2 91,673 + +0.6 91,704 - -2.3 91,712 - +1.2 91,727 + -0.0 91,727 + +0.5 91,727 + -1.8 91,727 + -0.0 91,727 + -1.1 91,727 + +0.9 91,728 - -2.4 91,728 - -0.2 91,728 - +0.2 91,768 + +0.5 91,768 + -1.2 91,768 + -3.5 91,769 - +0.7 91,769 - +2.7 91,769 - -0.6 91,799 + -0.1 91,799 + -0.8 91,800 - +0.9 91,800 - -0.2 91,800 - +1.0 91,817 + +1.2 91,817 + +0.3 91,817 + -0.8 91,817 + +1.6 91,817 + -2.4 91,818 - +0.6 91,818 - +1.9 91,818 - -0.0 91,830 + +0.9 91,847 + -1.8 91,854 - +1.3 91,868 - +1.0 91,884 + -0.5 91,884 + +1.5 91,884 + +0.6 91,885 - -1.3 91,885 - +1.7 91,885 - +0.4 91,885 - -1.2 91,885 - -1.2 91,885 - +0.5
Or see this region's nucleotide sequence