Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00625 and EX31_RS00630 are separated by 250 nucleotides EX31_RS00630 and EX31_RS00635 are separated by 95 nucleotides EX31_RS00635 and EX31_RS25035 overlap by 32 nucleotides EX31_RS25035 and EX31_RS00640 are separated by 202 nucleotides
EX31_RS00625: EX31_RS00625 - YgdI/YgdR family lipoprotein, at 130,604 to 130,822
_RS00625
EX31_RS00630: EX31_RS00630 - iron dicitrate ABC transporter ATP-binding protein, at 131,073 to 131,306
_RS00630
EX31_RS00635: EX31_RS00635 - hypothetical protein, at 131,402 to 131,620
_RS00635
EX31_RS25035: EX31_RS25035 - hypothetical protein, at 131,589 to 131,855
_RS25035
EX31_RS00640: EX31_RS00640 - FMN-dependent L-lactate dehydrogenase LldD, at 132,058 to 133,221
_RS00640
Position (kb) 131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 130.419 kb on + strand at 130.475 kb on + strand at 130.509 kb on + strand at 130.509 kb on + strand at 130.509 kb on + strand at 130.542 kb on + strand at 130.543 kb on - strand at 130.617 kb on + strand at 130.623 kb on + strand at 130.673 kb on + strand, within EX31_RS00625 at 130.673 kb on + strand, within EX31_RS00625 at 130.706 kb on - strand, within EX31_RS00625 at 130.847 kb on + strand at 130.854 kb on - strand at 130.857 kb on - strand at 130.882 kb on + strand at 130.882 kb on + strand at 130.999 kb on - strand at 131.064 kb on + strand at 131.077 kb on + strand at 131.077 kb on + strand at 131.078 kb on - strand at 131.078 kb on - strand at 131.157 kb on + strand, within EX31_RS00630 at 131.227 kb on + strand, within EX31_RS00630 at 131.227 kb on + strand, within EX31_RS00630 at 131.294 kb on + strand at 131.294 kb on + strand at 131.294 kb on + strand at 131.294 kb on + strand at 131.294 kb on + strand at 131.295 kb on - strand at 131.320 kb on - strand at 131.363 kb on + strand at 131.376 kb on + strand at 131.376 kb on + strand at 131.377 kb on - strand at 131.388 kb on - strand at 131.501 kb on - strand, within EX31_RS00635 at 131.544 kb on + strand, within EX31_RS00635 at 131.564 kb on + strand, within EX31_RS00635 at 131.575 kb on - strand, within EX31_RS00635 at 131.626 kb on + strand, within EX31_RS25035 at 131.643 kb on + strand, within EX31_RS25035 at 131.687 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.698 kb on - strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.771 kb on + strand, within EX31_RS25035 at 131.802 kb on + strand, within EX31_RS25035 at 131.824 kb on + strand, within EX31_RS25035 at 131.926 kb on + strand at 131.927 kb on - strand at 131.953 kb on - strand at 132.017 kb on - strand at 132.082 kb on + strand at 132.127 kb on + strand at 132.128 kb on - strand at 132.128 kb on - strand at 132.128 kb on - strand at 132.145 kb on + strand at 132.146 kb on - strand at 132.156 kb on + strand at 132.156 kb on + strand at 132.157 kb on - strand at 132.168 kb on + strand at 132.169 kb on - strand at 132.169 kb on - strand at 132.171 kb on + strand at 132.172 kb on - strand at 132.172 kb on - strand at 132.303 kb on + strand, within EX31_RS00640 at 132.303 kb on + strand, within EX31_RS00640 at 132.304 kb on - strand, within EX31_RS00640 at 132.307 kb on + strand, within EX31_RS00640 at 132.307 kb on + strand, within EX31_RS00640 at 132.307 kb on + strand, within EX31_RS00640 at 132.308 kb on - strand, within EX31_RS00640 at 132.308 kb on - strand, within EX31_RS00640 at 132.308 kb on - strand, within EX31_RS00640 at 132.442 kb on + strand, within EX31_RS00640 at 132.442 kb on + strand, within EX31_RS00640 at 132.442 kb on + strand, within EX31_RS00640 at 132.442 kb on + strand, within EX31_RS00640 at 132.442 kb on + strand, within EX31_RS00640 at 132.443 kb on - strand, within EX31_RS00640 at 132.519 kb on + strand, within EX31_RS00640
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 130,419 + -1.0 130,475 + +1.7 130,509 + -2.5 130,509 + +1.7 130,509 + +0.2 130,542 + -0.5 130,543 - +2.1 130,617 + -0.7 130,623 + -1.9 130,673 + EX31_RS00625 0.32 -1.9 130,673 + EX31_RS00625 0.32 +0.1 130,706 - EX31_RS00625 0.47 +1.1 130,847 + -0.5 130,854 - -1.9 130,857 - -0.5 130,882 + +0.9 130,882 + -0.5 130,999 - -1.2 131,064 + +0.5 131,077 + -1.7 131,077 + -0.4 131,078 - -1.5 131,078 - -1.2 131,157 + EX31_RS00630 0.36 +0.3 131,227 + EX31_RS00630 0.66 -2.1 131,227 + EX31_RS00630 0.66 +1.9 131,294 + -0.1 131,294 + +1.1 131,294 + +0.4 131,294 + -0.3 131,294 + -0.9 131,295 - -1.5 131,320 - -0.7 131,363 + +0.1 131,376 + +3.2 131,376 + -2.9 131,377 - -0.5 131,388 - +0.1 131,501 - EX31_RS00635 0.45 -2.6 131,544 + EX31_RS00635 0.65 -2.2 131,564 + EX31_RS00635 0.74 -1.5 131,575 - EX31_RS00635 0.79 +1.1 131,626 + EX31_RS25035 0.14 -0.5 131,643 + EX31_RS25035 0.20 -0.9 131,687 + EX31_RS25035 0.37 +1.1 131,697 + EX31_RS25035 0.40 +0.1 131,697 + EX31_RS25035 0.40 +0.3 131,697 + EX31_RS25035 0.40 +0.1 131,697 + EX31_RS25035 0.40 +0.0 131,697 + EX31_RS25035 0.40 -1.5 131,698 - EX31_RS25035 0.41 -0.2 131,699 + EX31_RS25035 0.41 +3.4 131,699 + EX31_RS25035 0.41 -0.5 131,699 + EX31_RS25035 0.41 +1.1 131,699 + EX31_RS25035 0.41 +0.7 131,699 + EX31_RS25035 0.41 +0.1 131,699 + EX31_RS25035 0.41 +1.7 131,771 + EX31_RS25035 0.68 +1.9 131,802 + EX31_RS25035 0.80 +2.3 131,824 + EX31_RS25035 0.88 -2.5 131,926 + -0.2 131,927 - -0.2 131,953 - -1.9 132,017 - -0.5 132,082 + -3.1 132,127 + -3.3 132,128 - -2.7 132,128 - -1.2 132,128 - -2.7 132,145 + -1.2 132,146 - +0.5 132,156 + -2.6 132,156 + -2.2 132,157 - -0.5 132,168 + +0.1 132,169 - -2.1 132,169 - -2.5 132,171 + -2.7 132,172 - +1.1 132,172 - +1.4 132,303 + EX31_RS00640 0.21 -1.5 132,303 + EX31_RS00640 0.21 +1.1 132,304 - EX31_RS00640 0.21 -3.0 132,307 + EX31_RS00640 0.21 +0.1 132,307 + EX31_RS00640 0.21 -2.8 132,307 + EX31_RS00640 0.21 -1.2 132,308 - EX31_RS00640 0.21 -1.7 132,308 - EX31_RS00640 0.21 -0.5 132,308 - EX31_RS00640 0.21 -1.5 132,442 + EX31_RS00640 0.33 -1.5 132,442 + EX31_RS00640 0.33 +1.1 132,442 + EX31_RS00640 0.33 -0.7 132,442 + EX31_RS00640 0.33 +1.1 132,442 + EX31_RS00640 0.33 -0.9 132,443 - EX31_RS00640 0.33 -0.9 132,519 + EX31_RS00640 0.40 -2.7
Or see this region's nucleotide sequence