Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12760 and CA265_RS12765 are separated by 37 nucleotides CA265_RS12765 and CA265_RS12770 are separated by 143 nucleotides
CA265_RS12760: CA265_RS12760 - orotate phosphoribosyltransferase, at 3,178,638 to 3,179,303
_RS12760
CA265_RS12765: CA265_RS12765 - SRPBCC family protein, at 3,179,341 to 3,179,739
_RS12765
CA265_RS12770: CA265_RS12770 - alpha-rhamnosidase, at 3,179,883 to 3,182,558
_RS12770
Position (kb)
3179
3180 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3178.372 kb on + strand at 3178.414 kb on + strand at 3178.415 kb on - strand at 3178.415 kb on - strand at 3178.416 kb on + strand at 3178.423 kb on + strand at 3178.424 kb on - strand at 3178.424 kb on - strand at 3178.436 kb on - strand at 3178.491 kb on - strand at 3178.526 kb on - strand at 3178.596 kb on + strand at 3179.284 kb on + strand at 3179.339 kb on + strand at 3179.409 kb on + strand, within CA265_RS12765 at 3179.442 kb on + strand, within CA265_RS12765 at 3179.449 kb on - strand, within CA265_RS12765 at 3179.449 kb on - strand, within CA265_RS12765 at 3179.585 kb on + strand, within CA265_RS12765 at 3179.597 kb on + strand, within CA265_RS12765 at 3179.597 kb on + strand, within CA265_RS12765 at 3179.598 kb on - strand, within CA265_RS12765 at 3179.623 kb on - strand, within CA265_RS12765 at 3179.638 kb on - strand, within CA265_RS12765 at 3179.687 kb on + strand, within CA265_RS12765 at 3179.688 kb on - strand, within CA265_RS12765 at 3179.759 kb on - strand at 3179.811 kb on + strand at 3179.851 kb on - strand at 3179.853 kb on + strand at 3179.899 kb on + strand at 3180.069 kb on + strand at 3180.142 kb on + strand at 3180.142 kb on + strand at 3180.197 kb on + strand, within CA265_RS12770 at 3180.204 kb on + strand, within CA265_RS12770 at 3180.205 kb on - strand, within CA265_RS12770 at 3180.205 kb on - strand, within CA265_RS12770 at 3180.205 kb on - strand, within CA265_RS12770 at 3180.205 kb on - strand, within CA265_RS12770 at 3180.249 kb on - strand, within CA265_RS12770 at 3180.352 kb on - strand, within CA265_RS12770 at 3180.368 kb on + strand, within CA265_RS12770 at 3180.402 kb on + strand, within CA265_RS12770 at 3180.402 kb on + strand, within CA265_RS12770 at 3180.424 kb on - strand, within CA265_RS12770 at 3180.502 kb on + strand, within CA265_RS12770 at 3180.503 kb on - strand, within CA265_RS12770 at 3180.510 kb on - strand, within CA265_RS12770 at 3180.513 kb on + strand, within CA265_RS12770 at 3180.513 kb on + strand, within CA265_RS12770 at 3180.580 kb on - strand, within CA265_RS12770 at 3180.602 kb on - strand, within CA265_RS12770 at 3180.633 kb on - strand, within CA265_RS12770 at 3180.663 kb on - strand, within CA265_RS12770 at 3180.721 kb on + strand, within CA265_RS12770 at 3180.738 kb on - strand, within CA265_RS12770
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 3,178,372 + +0.3 3,178,414 + -0.1 3,178,415 - -3.8 3,178,415 - -0.1 3,178,416 + -2.8 3,178,423 + +0.1 3,178,424 - +0.2 3,178,424 - +0.9 3,178,436 - +0.8 3,178,491 - -2.4 3,178,526 - +0.5 3,178,596 + -0.7 3,179,284 + -0.6 3,179,339 + -0.9 3,179,409 + CA265_RS12765 0.17 +0.0 3,179,442 + CA265_RS12765 0.25 +1.1 3,179,449 - CA265_RS12765 0.27 +0.4 3,179,449 - CA265_RS12765 0.27 +1.3 3,179,585 + CA265_RS12765 0.61 +0.7 3,179,597 + CA265_RS12765 0.64 +0.6 3,179,597 + CA265_RS12765 0.64 +0.5 3,179,598 - CA265_RS12765 0.64 +1.9 3,179,623 - CA265_RS12765 0.71 +0.7 3,179,638 - CA265_RS12765 0.74 +0.0 3,179,687 + CA265_RS12765 0.87 +0.6 3,179,688 - CA265_RS12765 0.87 +0.6 3,179,759 - +0.3 3,179,811 + -0.9 3,179,851 - -0.3 3,179,853 + +0.9 3,179,899 + +0.8 3,180,069 + -0.1 3,180,142 + -0.1 3,180,142 + +1.0 3,180,197 + CA265_RS12770 0.12 +0.5 3,180,204 + CA265_RS12770 0.12 +0.0 3,180,205 - CA265_RS12770 0.12 -1.1 3,180,205 - CA265_RS12770 0.12 -0.4 3,180,205 - CA265_RS12770 0.12 -1.7 3,180,205 - CA265_RS12770 0.12 +0.6 3,180,249 - CA265_RS12770 0.14 -2.0 3,180,352 - CA265_RS12770 0.18 -0.0 3,180,368 + CA265_RS12770 0.18 -0.1 3,180,402 + CA265_RS12770 0.19 +0.3 3,180,402 + CA265_RS12770 0.19 -0.0 3,180,424 - CA265_RS12770 0.20 -0.1 3,180,502 + CA265_RS12770 0.23 -0.4 3,180,503 - CA265_RS12770 0.23 +0.7 3,180,510 - CA265_RS12770 0.23 -1.5 3,180,513 + CA265_RS12770 0.24 -1.1 3,180,513 + CA265_RS12770 0.24 -0.0 3,180,580 - CA265_RS12770 0.26 -0.1 3,180,602 - CA265_RS12770 0.27 +1.0 3,180,633 - CA265_RS12770 0.28 -0.5 3,180,663 - CA265_RS12770 0.29 +2.5 3,180,721 + CA265_RS12770 0.31 -0.9 3,180,738 - CA265_RS12770 0.32 +0.7
Or see this region's nucleotide sequence