Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS36005

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35995 and BPHYT_RS36000 are separated by 242 nucleotidesBPHYT_RS36000 and BPHYT_RS36005 are separated by 151 nucleotidesBPHYT_RS36005 and BPHYT_RS36010 are separated by 63 nucleotidesBPHYT_RS36010 and BPHYT_RS36015 are separated by 58 nucleotidesBPHYT_RS36015 and BPHYT_RS36020 are separated by 181 nucleotides BPHYT_RS35995: BPHYT_RS35995 - hypothetical protein, at 32,185 to 32,418 _RS35995 BPHYT_RS36000: BPHYT_RS36000 - hypothetical protein, at 32,661 to 32,903 _RS36000 BPHYT_RS36005: BPHYT_RS36005 - hypothetical protein, at 33,055 to 33,375 _RS36005 BPHYT_RS36010: BPHYT_RS36010 - hypothetical protein, at 33,439 to 33,915 _RS36010 BPHYT_RS36015: BPHYT_RS36015 - hypothetical protein, at 33,974 to 34,153 _RS36015 BPHYT_RS36020: BPHYT_RS36020 - hypothetical protein, at 34,335 to 34,571 _RS36020 Position (kb) 33 34Strain fitness (log2 ratio) -3 -2 -1 0 1at 32.125 kb on + strandat 32.172 kb on - strandat 32.286 kb on + strand, within BPHYT_RS35995at 32.321 kb on + strand, within BPHYT_RS35995at 32.330 kb on + strand, within BPHYT_RS35995at 32.330 kb on + strand, within BPHYT_RS35995at 32.331 kb on - strand, within BPHYT_RS35995at 32.331 kb on - strand, within BPHYT_RS35995at 32.380 kb on + strand, within BPHYT_RS35995at 32.487 kb on + strandat 32.488 kb on - strandat 32.501 kb on - strandat 32.814 kb on - strand, within BPHYT_RS36000at 32.829 kb on - strand, within BPHYT_RS36000at 32.829 kb on - strand, within BPHYT_RS36000at 32.933 kb on - strandat 32.949 kb on + strandat 33.030 kb on + strandat 33.033 kb on + strandat 33.033 kb on + strandat 33.109 kb on + strand, within BPHYT_RS36005at 33.110 kb on - strand, within BPHYT_RS36005at 33.110 kb on - strand, within BPHYT_RS36005at 33.239 kb on - strandat 33.239 kb on - strand, within BPHYT_RS36005at 33.329 kb on - strand, within BPHYT_RS36005at 33.358 kb on + strandat 33.359 kb on - strandat 33.359 kb on - strandat 33.560 kb on + strand, within BPHYT_RS36010at 33.774 kb on + strand, within BPHYT_RS36010at 33.775 kb on - strand, within BPHYT_RS36010at 33.787 kb on + strand, within BPHYT_RS36010at 33.787 kb on + strand, within BPHYT_RS36010at 33.787 kb on + strand, within BPHYT_RS36010at 33.788 kb on - strand, within BPHYT_RS36010at 33.847 kb on + strand, within BPHYT_RS36010at 33.902 kb on + strandat 33.924 kb on - strandat 33.924 kb on - strandat 34.153 kb on + strandat 34.154 kb on - strandat 34.273 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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32,125 + +1.6
32,172 - -2.1
32,286 + BPHYT_RS35995 0.43 +0.1
32,321 + BPHYT_RS35995 0.58 +0.3
32,330 + BPHYT_RS35995 0.62 -0.3
32,330 + BPHYT_RS35995 0.62 -0.8
32,331 - BPHYT_RS35995 0.62 -1.6
32,331 - BPHYT_RS35995 0.62 -1.7
32,380 + BPHYT_RS35995 0.83 +0.2
32,487 + -1.6
32,488 - -0.8
32,501 - -1.1
32,814 - BPHYT_RS36000 0.63 -3.4
32,829 - BPHYT_RS36000 0.69 -2.3
32,829 - BPHYT_RS36000 0.69 -0.6
32,933 - -0.4
32,949 + +0.1
33,030 + -0.1
33,033 + +0.1
33,033 + +1.2
33,109 + BPHYT_RS36005 0.17 -1.3
33,110 - BPHYT_RS36005 0.17 -1.0
33,110 - BPHYT_RS36005 0.17 -2.5
33,239 - -1.6
33,239 - BPHYT_RS36005 0.57 -1.2
33,329 - BPHYT_RS36005 0.85 -1.3
33,358 + +0.8
33,359 - -1.8
33,359 - -1.0
33,560 + BPHYT_RS36010 0.25 +0.3
33,774 + BPHYT_RS36010 0.70 -0.7
33,775 - BPHYT_RS36010 0.70 -1.4
33,787 + BPHYT_RS36010 0.73 -0.7
33,787 + BPHYT_RS36010 0.73 +1.0
33,787 + BPHYT_RS36010 0.73 -2.1
33,788 - BPHYT_RS36010 0.73 -2.2
33,847 + BPHYT_RS36010 0.86 -0.6
33,902 + +0.5
33,924 - -1.0
33,924 - -1.6
34,153 + +1.5
34,154 - -2.1
34,273 - -3.7

Or see this region's nucleotide sequence