Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33255

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33250 and BPHYT_RS33255 overlap by 4 nucleotidesBPHYT_RS33255 and BPHYT_RS33260 are separated by 269 nucleotidesBPHYT_RS33260 and BPHYT_RS33265 are separated by 133 nucleotides BPHYT_RS33250: BPHYT_RS33250 - diguanylate phosphodiesterase, at 3,040,903 to 3,044,016 _RS33250 BPHYT_RS33255: BPHYT_RS33255 - ABC transporter substrate-binding protein, at 3,044,013 to 3,044,807 _RS33255 BPHYT_RS33260: BPHYT_RS33260 - hypothetical protein, at 3,045,077 to 3,045,505 _RS33260 BPHYT_RS33265: BPHYT_RS33265 - Catalase domain-containing protein, at 3,045,639 to 3,046,700 _RS33265 Position (kb) 3044 3045Strain fitness (log2 ratio) -1 0 1at 3043.181 kb on + strand, within BPHYT_RS33250at 3043.274 kb on + strand, within BPHYT_RS33250at 3043.310 kb on + strand, within BPHYT_RS33250at 3043.413 kb on - strand, within BPHYT_RS33250at 3043.413 kb on - strand, within BPHYT_RS33250at 3043.424 kb on + strand, within BPHYT_RS33250at 3043.425 kb on - strand, within BPHYT_RS33250at 3043.610 kb on + strand, within BPHYT_RS33250at 3043.611 kb on - strand, within BPHYT_RS33250at 3043.770 kb on - strandat 3043.892 kb on + strandat 3043.892 kb on + strandat 3044.303 kb on - strand, within BPHYT_RS33255at 3044.476 kb on + strand, within BPHYT_RS33255at 3044.479 kb on + strand, within BPHYT_RS33255at 3044.480 kb on - strand, within BPHYT_RS33255at 3044.480 kb on - strand, within BPHYT_RS33255at 3044.494 kb on + strand, within BPHYT_RS33255at 3044.512 kb on + strand, within BPHYT_RS33255at 3044.520 kb on - strand, within BPHYT_RS33255at 3044.520 kb on - strand, within BPHYT_RS33255at 3044.687 kb on - strand, within BPHYT_RS33255at 3044.707 kb on - strand, within BPHYT_RS33255at 3044.942 kb on - strandat 3044.942 kb on - strandat 3044.988 kb on - strandat 3044.995 kb on + strandat 3044.995 kb on + strandat 3045.094 kb on + strandat 3045.111 kb on + strandat 3045.111 kb on + strandat 3045.348 kb on - strand, within BPHYT_RS33260at 3045.475 kb on + strandat 3045.556 kb on - strandat 3045.731 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,043,181 + BPHYT_RS33250 0.73 +0.3
3,043,274 + BPHYT_RS33250 0.76 +1.5
3,043,310 + BPHYT_RS33250 0.77 -0.9
3,043,413 - BPHYT_RS33250 0.81 +0.6
3,043,413 - BPHYT_RS33250 0.81 +0.0
3,043,424 + BPHYT_RS33250 0.81 -0.7
3,043,425 - BPHYT_RS33250 0.81 +1.1
3,043,610 + BPHYT_RS33250 0.87 -0.8
3,043,611 - BPHYT_RS33250 0.87 +0.3
3,043,770 - +0.2
3,043,892 + +0.3
3,043,892 + +0.3
3,044,303 - BPHYT_RS33255 0.36 -0.6
3,044,476 + BPHYT_RS33255 0.58 +0.6
3,044,479 + BPHYT_RS33255 0.59 -0.4
3,044,480 - BPHYT_RS33255 0.59 -0.1
3,044,480 - BPHYT_RS33255 0.59 -0.1
3,044,494 + BPHYT_RS33255 0.61 +0.1
3,044,512 + BPHYT_RS33255 0.63 +0.7
3,044,520 - BPHYT_RS33255 0.64 -0.0
3,044,520 - BPHYT_RS33255 0.64 -0.4
3,044,687 - BPHYT_RS33255 0.85 +0.4
3,044,707 - BPHYT_RS33255 0.87 -1.1
3,044,942 - +0.4
3,044,942 - -0.7
3,044,988 - -0.9
3,044,995 + -0.1
3,044,995 + -0.5
3,045,094 + -1.0
3,045,111 + +1.1
3,045,111 + -0.4
3,045,348 - BPHYT_RS33260 0.63 +1.3
3,045,475 + -0.8
3,045,556 - +1.4
3,045,731 + +0.4

Or see this region's nucleotide sequence