Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32185 and BPHYT_RS32190 are separated by 1 nucleotides BPHYT_RS32190 and BPHYT_RS32195 are separated by 16 nucleotides BPHYT_RS32195 and BPHYT_RS32200 are separated by 41 nucleotides BPHYT_RS32200 and BPHYT_RS32205 are separated by 290 nucleotides BPHYT_RS32205 and BPHYT_RS32210 are separated by 377 nucleotides
BPHYT_RS32185: BPHYT_RS32185 - type IV secretion protein VirB4, at 2,793,237 to 2,795,702
_RS32185
BPHYT_RS32190: BPHYT_RS32190 - conjugal transfer protein TraD, at 2,795,704 to 2,796,048
_RS32190
BPHYT_RS32195: BPHYT_RS32195 - type VI secretion protein, at 2,796,065 to 2,796,406
_RS32195
BPHYT_RS32200: BPHYT_RS32200 - lytic transglycosylase, at 2,796,448 to 2,797,092
_RS32200
BPHYT_RS32205: BPHYT_RS32205 - hypothetical protein, at 2,797,383 to 2,797,622
_RS32205
BPHYT_RS32210: BPHYT_RS32210 - hypothetical protein, at 2,798,000 to 2,798,284
_RS32210
Position (kb)
2796
2797
2798 Strain fitness (log2 ratio)
-1
0
1 at 2795.587 kb on + strand at 2795.798 kb on + strand, within BPHYT_RS32190 at 2796.021 kb on + strand at 2796.025 kb on - strand at 2796.025 kb on - strand at 2796.601 kb on + strand, within BPHYT_RS32200 at 2796.657 kb on - strand, within BPHYT_RS32200 at 2797.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,795,587 + +0.3 2,795,798 + BPHYT_RS32190 0.27 +0.4 2,796,021 + +0.4 2,796,025 - -0.3 2,796,025 - -0.7 2,796,601 + BPHYT_RS32200 0.24 -0.4 2,796,657 - BPHYT_RS32200 0.32 -1.0 2,797,960 - +0.1
Or see this region's nucleotide sequence