Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28250

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28245 and BPHYT_RS28250 are separated by 97 nucleotidesBPHYT_RS28250 and BPHYT_RS28255 are separated by 491 nucleotidesBPHYT_RS28255 and BPHYT_RS28260 are separated by 120 nucleotides BPHYT_RS28245: BPHYT_RS28245 - MFS transporter, at 1,894,154 to 1,895,482 _RS28245 BPHYT_RS28250: BPHYT_RS28250 - 3-oxoacyl-ACP reductase, at 1,895,580 to 1,896,332 _RS28250 BPHYT_RS28255: BPHYT_RS28255 - hypothetical protein, at 1,896,824 to 1,897,033 _RS28255 BPHYT_RS28260: BPHYT_RS28260 - oxidoreductase, at 1,897,154 to 1,897,852 _RS28260 Position (kb) 1895 1896 1897Strain fitness (log2 ratio) -1 0 1at 1894.584 kb on + strand, within BPHYT_RS28245at 1895.569 kb on - strandat 1896.440 kb on + strandat 1896.440 kb on + strandat 1896.441 kb on - strandat 1896.897 kb on + strand, within BPHYT_RS28255

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,894,584 + BPHYT_RS28245 0.32 +0.4
1,895,569 - -0.9
1,896,440 + +0.5
1,896,440 + +0.7
1,896,441 - -1.2
1,896,897 + BPHYT_RS28255 0.35 +0.5

Or see this region's nucleotide sequence