Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21230 and BPHYT_RS21235 are separated by 154 nucleotides BPHYT_RS21235 and BPHYT_RS21240 overlap by 17 nucleotides BPHYT_RS21240 and BPHYT_RS21245 are separated by 688 nucleotides
BPHYT_RS21230: BPHYT_RS21230 - acyl-homoserine-lactone synthase, at 324,705 to 325,298
_RS21230
BPHYT_RS21235: BPHYT_RS21235 - hypothetical protein, at 325,453 to 325,770
_RS21235
BPHYT_RS21240: BPHYT_RS21240 - LuxR family transcriptional regulator, at 325,754 to 326,458
_RS21240
BPHYT_RS21245: BPHYT_RS21245 - gluconolactonase, at 327,147 to 328,088
_RS21245
Position (kb) 325
326
327 Strain fitness (log2 ratio) -1
0
1
2
3 at 324.878 kb on - strand, within BPHYT_RS21230 at 325.135 kb on + strand, within BPHYT_RS21230 at 325.136 kb on - strand, within BPHYT_RS21230 at 325.136 kb on - strand, within BPHYT_RS21230 at 325.191 kb on - strand, within BPHYT_RS21230 at 325.234 kb on + strand, within BPHYT_RS21230 at 325.235 kb on - strand, within BPHYT_RS21230 at 325.235 kb on - strand, within BPHYT_RS21230 at 325.235 kb on - strand, within BPHYT_RS21230 at 325.235 kb on - strand, within BPHYT_RS21230 at 325.235 kb on - strand, within BPHYT_RS21230 at 325.370 kb on + strand at 325.370 kb on + strand at 325.370 kb on + strand at 325.371 kb on - strand at 325.371 kb on - strand at 325.371 kb on - strand at 325.371 kb on - strand at 325.375 kb on - strand at 325.375 kb on - strand at 325.383 kb on - strand at 325.383 kb on - strand at 325.549 kb on + strand, within BPHYT_RS21235 at 325.675 kb on + strand, within BPHYT_RS21235 at 325.768 kb on + strand at 325.769 kb on - strand at 325.769 kb on - strand at 325.947 kb on - strand, within BPHYT_RS21240 at 325.947 kb on - strand, within BPHYT_RS21240 at 326.003 kb on + strand, within BPHYT_RS21240 at 326.048 kb on - strand, within BPHYT_RS21240 at 326.048 kb on - strand, within BPHYT_RS21240 at 326.172 kb on + strand, within BPHYT_RS21240 at 326.173 kb on - strand, within BPHYT_RS21240 at 326.286 kb on + strand, within BPHYT_RS21240 at 326.286 kb on + strand, within BPHYT_RS21240 at 326.287 kb on - strand, within BPHYT_RS21240 at 326.290 kb on + strand, within BPHYT_RS21240 at 326.290 kb on + strand, within BPHYT_RS21240 at 326.290 kb on + strand, within BPHYT_RS21240 at 326.310 kb on + strand, within BPHYT_RS21240 at 326.310 kb on + strand, within BPHYT_RS21240 at 326.310 kb on + strand at 326.310 kb on + strand, within BPHYT_RS21240 at 326.314 kb on + strand, within BPHYT_RS21240 at 326.314 kb on + strand, within BPHYT_RS21240 at 326.314 kb on + strand, within BPHYT_RS21240 at 326.315 kb on - strand, within BPHYT_RS21240 at 326.325 kb on + strand, within BPHYT_RS21240 at 326.326 kb on - strand, within BPHYT_RS21240 at 326.326 kb on - strand, within BPHYT_RS21240 at 326.326 kb on - strand, within BPHYT_RS21240 at 326.563 kb on + strand at 326.563 kb on + strand at 326.569 kb on - strand at 326.700 kb on + strand at 326.700 kb on + strand at 326.753 kb on - strand at 326.768 kb on + strand at 326.853 kb on + strand at 326.874 kb on + strand at 326.900 kb on + strand at 326.994 kb on + strand at 327.246 kb on + strand, within BPHYT_RS21245 at 327.247 kb on - strand, within BPHYT_RS21245 at 327.304 kb on + strand, within BPHYT_RS21245 at 327.304 kb on + strand, within BPHYT_RS21245 at 327.402 kb on - strand, within BPHYT_RS21245
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 324,878 - BPHYT_RS21230 0.29 +0.9 325,135 + BPHYT_RS21230 0.72 +1.0 325,136 - BPHYT_RS21230 0.73 +0.2 325,136 - BPHYT_RS21230 0.73 +0.4 325,191 - BPHYT_RS21230 0.82 -0.6 325,234 + BPHYT_RS21230 0.89 +1.0 325,235 - BPHYT_RS21230 0.89 -0.8 325,235 - BPHYT_RS21230 0.89 -0.1 325,235 - BPHYT_RS21230 0.89 +0.5 325,235 - BPHYT_RS21230 0.89 -0.1 325,235 - BPHYT_RS21230 0.89 -1.7 325,370 + -0.3 325,370 + -0.6 325,370 + +2.0 325,371 - +0.4 325,371 - +1.2 325,371 - +1.0 325,371 - +0.4 325,375 - +0.6 325,375 - +0.4 325,383 - +0.5 325,383 - +0.5 325,549 + BPHYT_RS21235 0.30 +0.8 325,675 + BPHYT_RS21235 0.70 +0.4 325,768 + +1.0 325,769 - +0.7 325,769 - +1.0 325,947 - BPHYT_RS21240 0.27 +1.4 325,947 - BPHYT_RS21240 0.27 +0.5 326,003 + BPHYT_RS21240 0.35 -0.6 326,048 - BPHYT_RS21240 0.42 -0.5 326,048 - BPHYT_RS21240 0.42 +0.1 326,172 + BPHYT_RS21240 0.59 +2.4 326,173 - BPHYT_RS21240 0.59 +1.4 326,286 + BPHYT_RS21240 0.75 +1.7 326,286 + BPHYT_RS21240 0.75 +0.1 326,287 - BPHYT_RS21240 0.76 -0.2 326,290 + BPHYT_RS21240 0.76 -0.1 326,290 + BPHYT_RS21240 0.76 +1.0 326,290 + BPHYT_RS21240 0.76 +0.2 326,310 + BPHYT_RS21240 0.79 +1.0 326,310 + BPHYT_RS21240 0.79 +0.2 326,310 + +1.0 326,310 + BPHYT_RS21240 0.79 +0.5 326,314 + BPHYT_RS21240 0.79 +1.1 326,314 + BPHYT_RS21240 0.79 +1.0 326,314 + BPHYT_RS21240 0.79 +1.5 326,315 - BPHYT_RS21240 0.80 -0.6 326,325 + BPHYT_RS21240 0.81 +0.8 326,326 - BPHYT_RS21240 0.81 -0.8 326,326 - BPHYT_RS21240 0.81 -0.4 326,326 - BPHYT_RS21240 0.81 +0.4 326,563 + +0.9 326,563 + -0.5 326,569 - -0.5 326,700 + +1.7 326,700 + +2.8 326,753 - -1.3 326,768 + +0.8 326,853 + +0.3 326,874 + +0.8 326,900 + -0.5 326,994 + +0.9 327,246 + BPHYT_RS21245 0.11 -0.2 327,247 - BPHYT_RS21245 0.11 -1.1 327,304 + BPHYT_RS21245 0.17 +0.5 327,304 + BPHYT_RS21245 0.17 +0.3 327,402 - BPHYT_RS21245 0.27 +0.1
Or see this region's nucleotide sequence