Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18980 and BPHYT_RS18985 overlap by 4 nucleotides BPHYT_RS18985 and BPHYT_RS18990 are separated by 16 nucleotides BPHYT_RS18990 and BPHYT_RS18995 are separated by 43 nucleotides BPHYT_RS18995 and BPHYT_RS19000 are separated by 69 nucleotides
BPHYT_RS18980: BPHYT_RS18980 - chemotaxis protein CheR, at 4,262,268 to 4,263,224
_RS18980
BPHYT_RS18985: BPHYT_RS18985 - hypothetical protein, at 4,263,221 to 4,263,412
_RS18985
BPHYT_RS18990: BPHYT_RS18990 - chemotaxis protein, at 4,263,429 to 4,265,282
_RS18990
BPHYT_RS18995: BPHYT_RS18995 - purine-binding chemotaxis protein, at 4,265,326 to 4,265,862
_RS18995
BPHYT_RS19000: BPHYT_RS19000 - chemotaxis protein CheA, at 4,265,932 to 4,268,232
_RS19000
Position (kb) 4263
4264
4265
4266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4262.504 kb on - strand, within BPHYT_RS18980 at 4262.704 kb on + strand, within BPHYT_RS18980 at 4262.705 kb on - strand, within BPHYT_RS18980 at 4262.705 kb on - strand, within BPHYT_RS18980 at 4263.058 kb on + strand, within BPHYT_RS18980 at 4263.059 kb on - strand, within BPHYT_RS18980 at 4263.243 kb on + strand, within BPHYT_RS18985 at 4263.243 kb on + strand, within BPHYT_RS18985 at 4263.283 kb on + strand, within BPHYT_RS18985 at 4263.284 kb on - strand, within BPHYT_RS18985 at 4263.430 kb on + strand at 4263.431 kb on - strand at 4263.590 kb on + strand at 4263.717 kb on - strand, within BPHYT_RS18990 at 4264.157 kb on + strand, within BPHYT_RS18990 at 4264.157 kb on + strand, within BPHYT_RS18990 at 4264.922 kb on + strand, within BPHYT_RS18990 at 4264.923 kb on - strand, within BPHYT_RS18990 at 4265.202 kb on + strand at 4265.244 kb on + strand at 4265.604 kb on + strand, within BPHYT_RS18995 at 4265.604 kb on + strand, within BPHYT_RS18995 at 4265.604 kb on + strand, within BPHYT_RS18995 at 4265.605 kb on - strand, within BPHYT_RS18995 at 4265.605 kb on - strand, within BPHYT_RS18995 at 4265.697 kb on - strand, within BPHYT_RS18995 at 4265.754 kb on + strand, within BPHYT_RS18995 at 4265.755 kb on - strand, within BPHYT_RS18995 at 4265.755 kb on - strand, within BPHYT_RS18995 at 4265.808 kb on + strand, within BPHYT_RS18995 at 4265.899 kb on + strand at 4265.899 kb on + strand at 4265.899 kb on + strand at 4265.899 kb on + strand at 4265.900 kb on - strand at 4265.900 kb on - strand at 4265.900 kb on - strand at 4265.906 kb on + strand at 4266.224 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.270 kb on - strand, within BPHYT_RS19000
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,262,504 - BPHYT_RS18980 0.25 -1.9 4,262,704 + BPHYT_RS18980 0.46 -1.6 4,262,705 - BPHYT_RS18980 0.46 -1.2 4,262,705 - BPHYT_RS18980 0.46 -2.9 4,263,058 + BPHYT_RS18980 0.83 -0.9 4,263,059 - BPHYT_RS18980 0.83 -1.0 4,263,243 + BPHYT_RS18985 0.11 -0.5 4,263,243 + BPHYT_RS18985 0.11 -4.1 4,263,283 + BPHYT_RS18985 0.32 -1.6 4,263,284 - BPHYT_RS18985 0.33 -0.7 4,263,430 + -2.3 4,263,431 - -0.8 4,263,590 + -1.3 4,263,717 - BPHYT_RS18990 0.16 -2.5 4,264,157 + BPHYT_RS18990 0.39 -2.2 4,264,157 + BPHYT_RS18990 0.39 -2.0 4,264,922 + BPHYT_RS18990 0.81 -2.6 4,264,923 - BPHYT_RS18990 0.81 -1.1 4,265,202 + -1.6 4,265,244 + +0.0 4,265,604 + BPHYT_RS18995 0.52 -1.5 4,265,604 + BPHYT_RS18995 0.52 -1.4 4,265,604 + BPHYT_RS18995 0.52 -0.9 4,265,605 - BPHYT_RS18995 0.52 -1.7 4,265,605 - BPHYT_RS18995 0.52 -1.2 4,265,697 - BPHYT_RS18995 0.69 -2.8 4,265,754 + BPHYT_RS18995 0.80 -1.1 4,265,755 - BPHYT_RS18995 0.80 -1.3 4,265,755 - BPHYT_RS18995 0.80 -1.3 4,265,808 + BPHYT_RS18995 0.90 -1.3 4,265,899 + -2.6 4,265,899 + -2.8 4,265,899 + -2.2 4,265,899 + -2.3 4,265,900 - -1.8 4,265,900 - -3.7 4,265,900 - -2.3 4,265,906 + -1.6 4,266,224 + BPHYT_RS19000 0.13 -2.9 4,266,269 + BPHYT_RS19000 0.15 -1.6 4,266,269 + BPHYT_RS19000 0.15 -3.0 4,266,269 + +1.9 4,266,269 + BPHYT_RS19000 0.15 -3.7 4,266,269 + BPHYT_RS19000 0.15 -0.1 4,266,269 + BPHYT_RS19000 0.15 -2.0 4,266,270 - BPHYT_RS19000 0.15 -2.3
Or see this region's nucleotide sequence