Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18920 and BPHYT_RS18925 are separated by 60 nucleotides BPHYT_RS18925 and BPHYT_RS18930 are separated by 28 nucleotides BPHYT_RS18930 and BPHYT_RS18935 overlap by 8 nucleotides
BPHYT_RS18920: BPHYT_RS18920 - hypothetical protein, at 4,249,125 to 4,249,535
_RS18920
BPHYT_RS18925: BPHYT_RS18925 - RNA polymerase sigma70, at 4,249,596 to 4,250,330
_RS18925
BPHYT_RS18930: BPHYT_RS18930 - flagellar biosynthesis protein FlhG, at 4,250,359 to 4,251,228
_RS18930
BPHYT_RS18935: BPHYT_RS18935 - flagellar biosynthesis regulator FlhF, at 4,251,221 to 4,253,146
_RS18935
Position (kb) 4250
4251
4252 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.461 kb on + strand, within BPHYT_RS18920 at 4249.738 kb on + strand, within BPHYT_RS18925 at 4249.738 kb on + strand, within BPHYT_RS18925 at 4249.739 kb on - strand, within BPHYT_RS18925 at 4249.903 kb on + strand, within BPHYT_RS18925 at 4249.906 kb on + strand, within BPHYT_RS18925 at 4249.907 kb on - strand, within BPHYT_RS18925 at 4249.907 kb on - strand, within BPHYT_RS18925 at 4249.948 kb on + strand, within BPHYT_RS18925 at 4249.949 kb on - strand, within BPHYT_RS18925 at 4249.949 kb on - strand, within BPHYT_RS18925 at 4250.131 kb on + strand, within BPHYT_RS18925 at 4250.165 kb on - strand, within BPHYT_RS18925 at 4250.281 kb on + strand at 4250.282 kb on - strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.463 kb on - strand, within BPHYT_RS18930 at 4250.463 kb on - strand, within BPHYT_RS18930 at 4250.577 kb on + strand, within BPHYT_RS18930 at 4250.578 kb on - strand, within BPHYT_RS18930 at 4250.609 kb on + strand, within BPHYT_RS18930 at 4250.647 kb on - strand, within BPHYT_RS18930 at 4250.711 kb on + strand, within BPHYT_RS18930 at 4250.712 kb on - strand, within BPHYT_RS18930 at 4250.712 kb on - strand, within BPHYT_RS18930 at 4250.736 kb on - strand, within BPHYT_RS18930 at 4251.070 kb on + strand, within BPHYT_RS18930 at 4251.222 kb on + strand at 4251.222 kb on + strand at 4251.223 kb on - strand at 4251.373 kb on - strand at 4251.408 kb on + strand at 4251.408 kb on + strand at 4251.409 kb on - strand at 4251.952 kb on - strand, within BPHYT_RS18935
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,249,411 + BPHYT_RS18920 0.70 +1.8 4,249,411 + BPHYT_RS18920 0.70 +0.6 4,249,415 + BPHYT_RS18920 0.71 -0.9 4,249,415 + BPHYT_RS18920 0.71 -0.5 4,249,421 + BPHYT_RS18920 0.72 +0.6 4,249,421 + BPHYT_RS18920 0.72 +0.5 4,249,461 + BPHYT_RS18920 0.82 -0.8 4,249,738 + BPHYT_RS18925 0.19 -2.9 4,249,738 + BPHYT_RS18925 0.19 -2.1 4,249,739 - BPHYT_RS18925 0.19 -3.1 4,249,903 + BPHYT_RS18925 0.42 -0.6 4,249,906 + BPHYT_RS18925 0.42 -0.9 4,249,907 - BPHYT_RS18925 0.42 -4.2 4,249,907 - BPHYT_RS18925 0.42 -1.6 4,249,948 + BPHYT_RS18925 0.48 -1.4 4,249,949 - BPHYT_RS18925 0.48 -3.0 4,249,949 - BPHYT_RS18925 0.48 -2.6 4,250,131 + BPHYT_RS18925 0.73 -0.6 4,250,165 - BPHYT_RS18925 0.77 -0.9 4,250,281 + +0.4 4,250,282 - -2.6 4,250,326 + -2.2 4,250,326 + -2.5 4,250,326 + +0.1 4,250,326 + -2.3 4,250,326 + -2.9 4,250,327 - -2.5 4,250,327 - -2.6 4,250,327 - -3.6 4,250,327 - -1.2 4,250,463 - BPHYT_RS18930 0.12 -1.1 4,250,463 - BPHYT_RS18930 0.12 -1.1 4,250,577 + BPHYT_RS18930 0.25 -2.8 4,250,578 - BPHYT_RS18930 0.25 -0.4 4,250,609 + BPHYT_RS18930 0.29 -3.8 4,250,647 - BPHYT_RS18930 0.33 -1.0 4,250,711 + BPHYT_RS18930 0.40 -1.4 4,250,712 - BPHYT_RS18930 0.41 -0.9 4,250,712 - BPHYT_RS18930 0.41 -0.9 4,250,736 - BPHYT_RS18930 0.43 +0.1 4,251,070 + BPHYT_RS18930 0.82 -2.8 4,251,222 + -1.9 4,251,222 + +0.8 4,251,223 - +0.2 4,251,373 - -4.3 4,251,408 + +0.4 4,251,408 + -1.8 4,251,409 - -1.5 4,251,952 - BPHYT_RS18935 0.38 -2.2
Or see this region's nucleotide sequence