Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17150 and BPHYT_RS17155 are separated by 47 nucleotides BPHYT_RS17155 and BPHYT_RS17160 are separated by 3 nucleotides BPHYT_RS17160 and BPHYT_RS17165 are separated by 194 nucleotides
BPHYT_RS17150: BPHYT_RS17150 - type II secretion system protein, at 3,885,740 to 3,886,969
_RS17150
BPHYT_RS17155: BPHYT_RS17155 - peptidase A24, at 3,887,017 to 3,887,937
_RS17155
BPHYT_RS17160: BPHYT_RS17160 - dephospho-CoA kinase, at 3,887,941 to 3,888,543
_RS17160
BPHYT_RS17165: BPHYT_RS17165 - cell division protein ZapD, at 3,888,738 to 3,889,493
_RS17165
Position (kb)
3887
3888 Strain fitness (log2 ratio)
-2
-1
0
1 at 3886.052 kb on - strand, within BPHYT_RS17150 at 3886.149 kb on - strand, within BPHYT_RS17150 at 3886.429 kb on - strand, within BPHYT_RS17150 at 3886.530 kb on + strand, within BPHYT_RS17150 at 3886.530 kb on + strand, within BPHYT_RS17150 at 3886.561 kb on - strand, within BPHYT_RS17150 at 3886.843 kb on - strand, within BPHYT_RS17150 at 3886.935 kb on - strand at 3886.935 kb on - strand at 3886.945 kb on + strand at 3886.946 kb on - strand at 3886.967 kb on + strand at 3886.967 kb on + strand at 3886.968 kb on - strand at 3886.992 kb on - strand at 3886.996 kb on + strand at 3887.005 kb on + strand at 3887.266 kb on + strand, within BPHYT_RS17155 at 3887.267 kb on - strand, within BPHYT_RS17155 at 3887.267 kb on - strand, within BPHYT_RS17155 at 3887.347 kb on + strand, within BPHYT_RS17155 at 3887.347 kb on + strand, within BPHYT_RS17155 at 3887.348 kb on - strand, within BPHYT_RS17155 at 3887.351 kb on + strand, within BPHYT_RS17155 at 3887.351 kb on + strand, within BPHYT_RS17155 at 3887.351 kb on + strand, within BPHYT_RS17155 at 3887.351 kb on + strand, within BPHYT_RS17155 at 3887.351 kb on + strand, within BPHYT_RS17155 at 3887.352 kb on - strand, within BPHYT_RS17155 at 3887.352 kb on - strand, within BPHYT_RS17155 at 3887.499 kb on + strand, within BPHYT_RS17155 at 3887.500 kb on - strand, within BPHYT_RS17155 at 3887.601 kb on + strand, within BPHYT_RS17155 at 3887.675 kb on + strand, within BPHYT_RS17155 at 3887.675 kb on + strand, within BPHYT_RS17155 at 3887.676 kb on - strand, within BPHYT_RS17155 at 3887.687 kb on + strand, within BPHYT_RS17155 at 3887.687 kb on + strand, within BPHYT_RS17155 at 3887.727 kb on - strand, within BPHYT_RS17155 at 3888.677 kb on - strand at 3888.690 kb on + strand at 3888.691 kb on - strand at 3888.753 kb on + strand at 3888.754 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,886,052 - BPHYT_RS17150 0.25 -1.5 3,886,149 - BPHYT_RS17150 0.33 +0.4 3,886,429 - BPHYT_RS17150 0.56 -0.3 3,886,530 + BPHYT_RS17150 0.64 -1.2 3,886,530 + BPHYT_RS17150 0.64 +0.7 3,886,561 - BPHYT_RS17150 0.67 -2.1 3,886,843 - BPHYT_RS17150 0.90 +0.1 3,886,935 - -0.9 3,886,935 - -0.0 3,886,945 + -0.2 3,886,946 - +0.2 3,886,967 + +0.7 3,886,967 + +0.7 3,886,968 - -1.0 3,886,992 - +0.4 3,886,996 + +0.0 3,887,005 + -0.2 3,887,266 + BPHYT_RS17155 0.27 -0.5 3,887,267 - BPHYT_RS17155 0.27 -0.2 3,887,267 - BPHYT_RS17155 0.27 +0.1 3,887,347 + BPHYT_RS17155 0.36 -0.6 3,887,347 + BPHYT_RS17155 0.36 -0.3 3,887,348 - BPHYT_RS17155 0.36 -0.8 3,887,351 + BPHYT_RS17155 0.36 -0.9 3,887,351 + BPHYT_RS17155 0.36 -0.2 3,887,351 + BPHYT_RS17155 0.36 -1.8 3,887,351 + BPHYT_RS17155 0.36 -1.0 3,887,351 + BPHYT_RS17155 0.36 -1.3 3,887,352 - BPHYT_RS17155 0.36 -0.6 3,887,352 - BPHYT_RS17155 0.36 -1.4 3,887,499 + BPHYT_RS17155 0.52 -0.4 3,887,500 - BPHYT_RS17155 0.52 +0.1 3,887,601 + BPHYT_RS17155 0.63 -1.2 3,887,675 + BPHYT_RS17155 0.71 +1.0 3,887,675 + BPHYT_RS17155 0.71 -0.4 3,887,676 - BPHYT_RS17155 0.72 -1.2 3,887,687 + BPHYT_RS17155 0.73 +0.7 3,887,687 + BPHYT_RS17155 0.73 -1.0 3,887,727 - BPHYT_RS17155 0.77 -0.2 3,888,677 - -2.2 3,888,690 + +0.0 3,888,691 - +0.1 3,888,753 + +0.6 3,888,754 - -2.5
Or see this region's nucleotide sequence