Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15380 and BPHYT_RS15385 are separated by 15 nucleotides BPHYT_RS15385 and BPHYT_RS15390 are separated by 201 nucleotides BPHYT_RS15390 and BPHYT_RS15395 are separated by 49 nucleotides BPHYT_RS15395 and BPHYT_RS15400 overlap by 4 nucleotides
BPHYT_RS15380: BPHYT_RS15380 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 3,491,753 to 3,492,904
_RS15380
BPHYT_RS15385: BPHYT_RS15385 - riboflavin synthase subunit alpha, at 3,492,920 to 3,493,534
_RS15385
BPHYT_RS15390: BPHYT_RS15390 - 3,4-Dihydroxy-2-butanone 4-phosphate synthase, at 3,493,736 to 3,494,872
_RS15390
BPHYT_RS15395: BPHYT_RS15395 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,494,922 to 3,495,428
_RS15395
BPHYT_RS15400: BPHYT_RS15400 - N utilization substance protein B homolog, at 3,495,425 to 3,495,859
_RS15400
Position (kb)
3493
3494
3495 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3492.850 kb on + strand at 3492.913 kb on + strand at 3493.532 kb on + strand at 3493.533 kb on - strand at 3493.533 kb on - strand at 3493.552 kb on + strand at 3493.552 kb on + strand at 3493.558 kb on + strand at 3493.558 kb on + strand at 3493.558 kb on + strand at 3493.559 kb on - strand at 3493.559 kb on - strand at 3493.559 kb on - strand at 3493.621 kb on + strand at 3493.622 kb on - strand at 3493.662 kb on + strand at 3493.703 kb on - strand at 3493.707 kb on + strand at 3493.755 kb on + strand at 3493.755 kb on + strand at 3494.327 kb on + strand, within BPHYT_RS15390 at 3494.411 kb on + strand, within BPHYT_RS15390 at 3494.411 kb on + strand, within BPHYT_RS15390 at 3494.714 kb on + strand, within BPHYT_RS15390 at 3494.714 kb on + strand, within BPHYT_RS15390 at 3494.736 kb on + strand, within BPHYT_RS15390 at 3494.736 kb on + strand, within BPHYT_RS15390 at 3495.474 kb on - strand, within BPHYT_RS15400
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,492,850 + -0.4 3,492,913 + +2.7 3,493,532 + +0.5 3,493,533 - -0.6 3,493,533 - -1.4 3,493,552 + -1.0 3,493,552 + +0.3 3,493,558 + -0.0 3,493,558 + -0.7 3,493,558 + +0.2 3,493,559 - -0.6 3,493,559 - -0.6 3,493,559 - -0.6 3,493,621 + +0.0 3,493,622 - -0.4 3,493,662 + -0.8 3,493,703 - -2.5 3,493,707 + +0.0 3,493,755 + -0.1 3,493,755 + -2.1 3,494,327 + BPHYT_RS15390 0.52 -0.8 3,494,411 + BPHYT_RS15390 0.59 +0.2 3,494,411 + BPHYT_RS15390 0.59 +0.2 3,494,714 + BPHYT_RS15390 0.86 -0.4 3,494,714 + BPHYT_RS15390 0.86 -2.0 3,494,736 + BPHYT_RS15390 0.88 +0.5 3,494,736 + BPHYT_RS15390 0.88 -0.3 3,495,474 - BPHYT_RS15400 0.11 +0.0
Or see this region's nucleotide sequence