Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14860

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14855 and BPHYT_RS14860 are separated by 148 nucleotidesBPHYT_RS14860 and BPHYT_RS14865 are separated by 49 nucleotides BPHYT_RS14855: BPHYT_RS14855 - ADP-L-glycero-D-manno-heptose-6-epimerase, at 3,378,748 to 3,379,740 _RS14855 BPHYT_RS14860: BPHYT_RS14860 - sugar kinase, at 3,379,889 to 3,380,809 _RS14860 BPHYT_RS14865: BPHYT_RS14865 - UDP-glucose 6-dehydrogenase, at 3,380,859 to 3,382,262 _RS14865 Position (kb) 3379 3380 3381Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3379.212 kb on + strand, within BPHYT_RS14855at 3379.232 kb on + strand, within BPHYT_RS14855at 3379.590 kb on - strand, within BPHYT_RS14855at 3380.893 kb on + strandat 3381.608 kb on - strand, within BPHYT_RS14865at 3381.641 kb on - strand, within BPHYT_RS14865

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,379,212 + BPHYT_RS14855 0.47 -4.0
3,379,232 + BPHYT_RS14855 0.49 -2.3
3,379,590 - BPHYT_RS14855 0.85 -2.4
3,380,893 + -5.3
3,381,608 - BPHYT_RS14865 0.53 -3.0
3,381,641 - BPHYT_RS14865 0.56 -4.2

Or see this region's nucleotide sequence