Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12215

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12210 and BPHYT_RS12215 are separated by 11 nucleotidesBPHYT_RS12215 and BPHYT_RS12220 are separated by 134 nucleotides BPHYT_RS12210: BPHYT_RS12210 - cardiolipin synthetase, at 2,787,907 to 2,789,358 _RS12210 BPHYT_RS12215: BPHYT_RS12215 - glutathione peroxidase, at 2,789,370 to 2,789,849 _RS12215 BPHYT_RS12220: BPHYT_RS12220 - glutathione ABC transporter ATP-binding protein, at 2,789,984 to 2,791,936 _RS12220 Position (kb) 2789 2790Strain fitness (log2 ratio) -2 -1 0 1 2at 2788.432 kb on + strand, within BPHYT_RS12210at 2788.478 kb on - strand, within BPHYT_RS12210at 2788.576 kb on - strand, within BPHYT_RS12210at 2788.576 kb on - strand, within BPHYT_RS12210at 2788.576 kb on - strand, within BPHYT_RS12210at 2788.636 kb on + strand, within BPHYT_RS12210at 2788.637 kb on - strand, within BPHYT_RS12210at 2788.637 kb on - strand, within BPHYT_RS12210at 2789.047 kb on + strand, within BPHYT_RS12210at 2789.048 kb on - strand, within BPHYT_RS12210at 2789.053 kb on + strand, within BPHYT_RS12210at 2789.054 kb on - strand, within BPHYT_RS12210at 2789.372 kb on - strandat 2789.425 kb on + strand, within BPHYT_RS12215at 2789.425 kb on + strand, within BPHYT_RS12215at 2789.695 kb on + strand, within BPHYT_RS12215at 2789.695 kb on + strand, within BPHYT_RS12215at 2789.696 kb on - strand, within BPHYT_RS12215at 2789.696 kb on - strand, within BPHYT_RS12215at 2789.704 kb on + strand, within BPHYT_RS12215at 2789.704 kb on + strand, within BPHYT_RS12215at 2789.786 kb on - strandat 2790.767 kb on + strand, within BPHYT_RS12220at 2790.767 kb on + strand, within BPHYT_RS12220at 2790.767 kb on + strand, within BPHYT_RS12220

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,788,432 + BPHYT_RS12210 0.36 +0.8
2,788,478 - BPHYT_RS12210 0.39 -0.1
2,788,576 - BPHYT_RS12210 0.46 -1.0
2,788,576 - BPHYT_RS12210 0.46 -1.8
2,788,576 - BPHYT_RS12210 0.46 -0.5
2,788,636 + BPHYT_RS12210 0.50 -0.2
2,788,637 - BPHYT_RS12210 0.50 -0.1
2,788,637 - BPHYT_RS12210 0.50 -0.7
2,789,047 + BPHYT_RS12210 0.79 -0.2
2,789,048 - BPHYT_RS12210 0.79 -0.5
2,789,053 + BPHYT_RS12210 0.79 -0.1
2,789,054 - BPHYT_RS12210 0.79 +0.1
2,789,372 - -0.7
2,789,425 + BPHYT_RS12215 0.11 +0.1
2,789,425 + BPHYT_RS12215 0.11 -1.8
2,789,695 + BPHYT_RS12215 0.68 +0.3
2,789,695 + BPHYT_RS12215 0.68 +0.7
2,789,696 - BPHYT_RS12215 0.68 +0.2
2,789,696 - BPHYT_RS12215 0.68 +0.4
2,789,704 + BPHYT_RS12215 0.70 +1.4
2,789,704 + BPHYT_RS12215 0.70 +0.6
2,789,786 - +1.8
2,790,767 + BPHYT_RS12220 0.40 +1.8
2,790,767 + BPHYT_RS12220 0.40 +1.7
2,790,767 + BPHYT_RS12220 0.40 -0.6

Or see this region's nucleotide sequence