Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10690 and BPHYT_RS10695 overlap by 4 nucleotides BPHYT_RS10695 and BPHYT_RS10700 are separated by 32 nucleotides BPHYT_RS10700 and BPHYT_RS10705 are separated by 40 nucleotides BPHYT_RS10705 and BPHYT_RS10710 are separated by 10 nucleotides
BPHYT_RS10690: BPHYT_RS10690 - Rieske (2Fe-2S) protein, at 2,433,692 to 2,434,975
_RS10690
BPHYT_RS10695: BPHYT_RS10695 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 2,434,972 to 2,435,472
_RS10695
BPHYT_RS10700: BPHYT_RS10700 - 4-oxalocrotonate tautomerase, at 2,435,505 to 2,435,888
_RS10700
BPHYT_RS10705: BPHYT_RS10705 - MFS transporter, at 2,435,929 to 2,437,245
_RS10705
BPHYT_RS10710: BPHYT_RS10710 - sensor histidine kinase, at 2,437,256 to 2,438,776
_RS10710
Position (kb)
2435
2436
2437
2438 Strain fitness (log2 ratio)
-2
-1
0
1 at 2435.108 kb on - strand, within BPHYT_RS10695 at 2435.510 kb on + strand at 2435.511 kb on - strand at 2436.430 kb on + strand, within BPHYT_RS10705 at 2436.877 kb on + strand, within BPHYT_RS10705 at 2436.878 kb on - strand, within BPHYT_RS10705 at 2436.879 kb on + strand, within BPHYT_RS10705 at 2437.132 kb on + strand at 2437.132 kb on + strand at 2437.227 kb on + strand at 2437.645 kb on - strand, within BPHYT_RS10710 at 2437.980 kb on + strand, within BPHYT_RS10710
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,435,108 - BPHYT_RS10695 0.27 +1.3 2,435,510 + -0.0 2,435,511 - -1.8 2,436,430 + BPHYT_RS10705 0.38 -0.2 2,436,877 + BPHYT_RS10705 0.72 -1.3 2,436,878 - BPHYT_RS10705 0.72 +1.0 2,436,879 + BPHYT_RS10705 0.72 -2.1 2,437,132 + +0.1 2,437,132 + -0.8 2,437,227 + +1.4 2,437,645 - BPHYT_RS10710 0.26 -0.8 2,437,980 + BPHYT_RS10710 0.48 -1.7
Or see this region's nucleotide sequence