Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08070

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08065 and BPHYT_RS08070 are separated by 93 nucleotidesBPHYT_RS08070 and BPHYT_RS08075 are separated by 215 nucleotidesBPHYT_RS08075 and BPHYT_RS08080 are separated by 94 nucleotides BPHYT_RS08065: BPHYT_RS08065 - transcription termination factor NusA, at 1,838,235 to 1,839,710 _RS08065 BPHYT_RS08070: BPHYT_RS08070 - translation initiation factor IF-2, at 1,839,804 to 1,842,764 _RS08070 BPHYT_RS08075: BPHYT_RS08075 - ribosome-binding factor A, at 1,842,980 to 1,843,345 _RS08075 BPHYT_RS08080: BPHYT_RS08080 - tRNA pseudouridine synthase B, at 1,843,440 to 1,844,375 _RS08080 Position (kb) 1839 1840 1841 1842 1843Strain fitness (log2 ratio) -1 0 1at 1839.729 kb on + strandat 1842.755 kb on + strandat 1842.755 kb on + strandat 1843.412 kb on - strandat 1843.412 kb on - strandat 1843.421 kb on - strandat 1843.554 kb on + strand, within BPHYT_RS08080at 1843.667 kb on + strand, within BPHYT_RS08080at 1843.751 kb on - strand, within BPHYT_RS08080

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,839,729 + -1.1
1,842,755 + -0.5
1,842,755 + +0.3
1,843,412 - -1.3
1,843,412 - +0.3
1,843,421 - +1.2
1,843,554 + BPHYT_RS08080 0.12 -0.6
1,843,667 + BPHYT_RS08080 0.24 +1.1
1,843,751 - BPHYT_RS08080 0.33 +0.0

Or see this region's nucleotide sequence