Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS07225

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS07215 and BPHYT_RS07220 are separated by 105 nucleotidesBPHYT_RS07220 and BPHYT_RS07225 are separated by 211 nucleotidesBPHYT_RS07225 and BPHYT_RS07230 are separated by 202 nucleotides BPHYT_RS07215: BPHYT_RS07215 - LysR family transcriptional regulator, at 1,639,849 to 1,640,754 _RS07215 BPHYT_RS07220: BPHYT_RS07220 - GCN5 family acetyltransferase, at 1,640,860 to 1,641,489 _RS07220 BPHYT_RS07225: BPHYT_RS07225 - membrane protein, at 1,641,701 to 1,641,862 _RS07225 BPHYT_RS07230: BPHYT_RS07230 - LysR family transcriptional regulator, at 1,642,065 to 1,643,042 _RS07230 Position (kb) 1641 1642Strain fitness (log2 ratio) -1 0 1at 1640.895 kb on + strandat 1640.902 kb on + strandat 1640.903 kb on - strandat 1641.161 kb on + strand, within BPHYT_RS07220at 1641.162 kb on - strand, within BPHYT_RS07220at 1641.891 kb on + strandat 1641.966 kb on - strandat 1642.141 kb on + strandat 1642.338 kb on - strand, within BPHYT_RS07230at 1642.779 kb on - strand, within BPHYT_RS07230at 1642.826 kb on + strand, within BPHYT_RS07230

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,640,895 + -0.1
1,640,902 + -0.3
1,640,903 - +0.6
1,641,161 + BPHYT_RS07220 0.48 -0.2
1,641,162 - BPHYT_RS07220 0.48 +0.5
1,641,891 + +0.4
1,641,966 - -0.6
1,642,141 + +1.0
1,642,338 - BPHYT_RS07230 0.28 -0.4
1,642,779 - BPHYT_RS07230 0.73 +0.4
1,642,826 + BPHYT_RS07230 0.78 +1.3

Or see this region's nucleotide sequence