Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04305 and BPHYT_RS04310 are separated by 85 nucleotides BPHYT_RS04310 and BPHYT_RS04315 are separated by 27 nucleotides
BPHYT_RS04305: BPHYT_RS04305 - hypothetical protein, at 987,528 to 989,003
_RS04305
BPHYT_RS04310: BPHYT_RS04310 - group 1 glycosyl transferase, at 989,089 to 993,141
_RS04310
BPHYT_RS04315: BPHYT_RS04315 - UDP-N-acetylglucosamine 2-epimerase, at 993,169 to 994,305
_RS04315
Position (kb) 989
990
991
992
993
994 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 988.520 kb on + strand, within BPHYT_RS04305 at 988.581 kb on - strand, within BPHYT_RS04305 at 988.632 kb on + strand, within BPHYT_RS04305 at 988.674 kb on + strand, within BPHYT_RS04305 at 988.675 kb on - strand, within BPHYT_RS04305 at 989.001 kb on + strand at 989.001 kb on + strand at 989.001 kb on + strand at 989.002 kb on - strand at 989.089 kb on - strand at 989.167 kb on + strand at 989.167 kb on + strand at 989.168 kb on - strand at 989.664 kb on + strand, within BPHYT_RS04310 at 989.774 kb on - strand, within BPHYT_RS04310 at 989.774 kb on - strand, within BPHYT_RS04310 at 990.286 kb on + strand, within BPHYT_RS04310 at 990.550 kb on + strand, within BPHYT_RS04310 at 990.558 kb on + strand, within BPHYT_RS04310 at 990.564 kb on + strand, within BPHYT_RS04310 at 990.564 kb on + strand, within BPHYT_RS04310 at 990.607 kb on + strand, within BPHYT_RS04310 at 990.772 kb on + strand, within BPHYT_RS04310 at 990.773 kb on - strand, within BPHYT_RS04310 at 990.940 kb on + strand, within BPHYT_RS04310 at 990.940 kb on + strand, within BPHYT_RS04310 at 990.941 kb on - strand, within BPHYT_RS04310 at 990.997 kb on + strand, within BPHYT_RS04310 at 991.365 kb on + strand, within BPHYT_RS04310 at 991.845 kb on + strand, within BPHYT_RS04310 at 992.205 kb on + strand, within BPHYT_RS04310 at 992.206 kb on - strand, within BPHYT_RS04310 at 992.212 kb on + strand, within BPHYT_RS04310 at 992.212 kb on + strand, within BPHYT_RS04310 at 992.213 kb on - strand, within BPHYT_RS04310 at 992.213 kb on - strand, within BPHYT_RS04310 at 992.275 kb on + strand, within BPHYT_RS04310 at 992.863 kb on + strand at 993.161 kb on + strand at 993.162 kb on - strand at 993.165 kb on + strand at 993.165 kb on + strand at 993.166 kb on - strand at 993.166 kb on - strand at 993.186 kb on - strand at 993.693 kb on + strand, within BPHYT_RS04315 at 993.713 kb on - strand, within BPHYT_RS04315 at 993.713 kb on - strand, within BPHYT_RS04315 at 993.895 kb on - strand, within BPHYT_RS04315 at 993.912 kb on + strand, within BPHYT_RS04315 at 993.913 kb on - strand, within BPHYT_RS04315 at 993.970 kb on + strand, within BPHYT_RS04315 at 993.971 kb on - strand, within BPHYT_RS04315 at 993.991 kb on + strand, within BPHYT_RS04315 at 994.029 kb on + strand, within BPHYT_RS04315 at 994.030 kb on - strand, within BPHYT_RS04315
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 988,520 + BPHYT_RS04305 0.67 +0.4 988,581 - BPHYT_RS04305 0.71 -1.3 988,632 + BPHYT_RS04305 0.75 -0.8 988,674 + BPHYT_RS04305 0.78 +1.1 988,675 - BPHYT_RS04305 0.78 +0.3 989,001 + -1.1 989,001 + -0.3 989,001 + -0.7 989,002 - -0.5 989,089 - +1.3 989,167 + -1.0 989,167 + -0.9 989,168 - -0.5 989,664 + BPHYT_RS04310 0.14 -1.9 989,774 - BPHYT_RS04310 0.17 -2.1 989,774 - BPHYT_RS04310 0.17 -1.4 990,286 + BPHYT_RS04310 0.30 +0.3 990,550 + BPHYT_RS04310 0.36 -0.4 990,558 + BPHYT_RS04310 0.36 -0.2 990,564 + BPHYT_RS04310 0.36 +0.8 990,564 + BPHYT_RS04310 0.36 +0.6 990,607 + BPHYT_RS04310 0.37 -0.3 990,772 + BPHYT_RS04310 0.42 -1.3 990,773 - BPHYT_RS04310 0.42 -2.4 990,940 + BPHYT_RS04310 0.46 +0.1 990,940 + BPHYT_RS04310 0.46 +2.9 990,941 - BPHYT_RS04310 0.46 -1.6 990,997 + BPHYT_RS04310 0.47 -0.1 991,365 + BPHYT_RS04310 0.56 -1.8 991,845 + BPHYT_RS04310 0.68 -1.7 992,205 + BPHYT_RS04310 0.77 -0.8 992,206 - BPHYT_RS04310 0.77 -2.5 992,212 + BPHYT_RS04310 0.77 -1.3 992,212 + BPHYT_RS04310 0.77 -0.2 992,213 - BPHYT_RS04310 0.77 -2.0 992,213 - BPHYT_RS04310 0.77 -0.8 992,275 + BPHYT_RS04310 0.79 -1.6 992,863 + -1.6 993,161 + +0.1 993,162 - +1.0 993,165 + -0.0 993,165 + +0.4 993,166 - +0.6 993,166 - -0.1 993,186 - +0.3 993,693 + BPHYT_RS04315 0.46 -1.0 993,713 - BPHYT_RS04315 0.48 -0.6 993,713 - BPHYT_RS04315 0.48 -0.5 993,895 - BPHYT_RS04315 0.64 +0.4 993,912 + BPHYT_RS04315 0.65 -1.0 993,913 - BPHYT_RS04315 0.65 +0.4 993,970 + BPHYT_RS04315 0.70 -0.2 993,971 - BPHYT_RS04315 0.71 -0.9 993,991 + BPHYT_RS04315 0.72 +0.4 994,029 + BPHYT_RS04315 0.76 -0.1 994,030 - BPHYT_RS04315 0.76 +2.0
Or see this region's nucleotide sequence