Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04205 and BPHYT_RS04210 are separated by 289 nucleotides BPHYT_RS04210 and BPHYT_RS04215 are separated by 255 nucleotides BPHYT_RS04215 and BPHYT_RS04220 are separated by 253 nucleotides BPHYT_RS04220 and BPHYT_RS04225 overlap by 4 nucleotides
BPHYT_RS04205: BPHYT_RS04205 - kinase, at 964,746 to 965,606
_RS04205
BPHYT_RS04210: BPHYT_RS04210 - rubredoxin, at 965,896 to 966,066
_RS04210
BPHYT_RS04215: BPHYT_RS04215 - hypothetical protein, at 966,322 to 967,734
_RS04215
BPHYT_RS04220: BPHYT_RS04220 - hypothetical protein, at 967,988 to 968,566
_RS04220
BPHYT_RS04225: BPHYT_RS04225 - Holliday junction resolvase, at 968,563 to 969,003
_RS04225
Position (kb) 966
967
968 Strain fitness (log2 ratio) -2
-1
0
1 at 965.663 kb on + strand at 965.663 kb on + strand at 965.664 kb on - strand at 965.805 kb on - strand at 965.879 kb on + strand at 966.228 kb on + strand at 966.228 kb on + strand at 966.229 kb on - strand at 966.947 kb on - strand, within BPHYT_RS04215 at 967.190 kb on - strand, within BPHYT_RS04215 at 967.336 kb on + strand, within BPHYT_RS04215 at 967.336 kb on + strand, within BPHYT_RS04215 at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.821 kb on - strand at 967.821 kb on - strand at 967.821 kb on - strand at 967.876 kb on + strand at 967.877 kb on - strand at 967.888 kb on + strand at 967.888 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.988 kb on - strand at 968.081 kb on + strand, within BPHYT_RS04220 at 968.213 kb on + strand, within BPHYT_RS04220 at 968.213 kb on + strand, within BPHYT_RS04220 at 968.279 kb on + strand, within BPHYT_RS04220
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 965,663 + +0.1 965,663 + -0.9 965,664 - +0.6 965,805 - -1.4 965,879 + +0.1 966,228 + +0.5 966,228 + +0.2 966,229 - +0.5 966,947 - BPHYT_RS04215 0.44 +0.6 967,190 - BPHYT_RS04215 0.61 +1.4 967,336 + BPHYT_RS04215 0.72 +0.5 967,336 + BPHYT_RS04215 0.72 -0.3 967,820 + -0.1 967,820 + -0.9 967,820 + -0.8 967,820 + -0.8 967,820 + -2.3 967,821 - +0.4 967,821 - +0.1 967,821 - -0.5 967,876 + -1.0 967,877 - +0.6 967,888 + +0.5 967,888 + -0.3 967,987 + +0.7 967,987 + +0.8 967,987 + +0.5 967,987 + -0.6 967,988 - -1.1 968,081 + BPHYT_RS04220 0.16 +0.4 968,213 + BPHYT_RS04220 0.39 -2.7 968,213 + BPHYT_RS04220 0.39 -0.3 968,279 + BPHYT_RS04220 0.50 -0.6
Or see this region's nucleotide sequence