Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03405 and BPHYT_RS03410 are separated by 162 nucleotides BPHYT_RS03410 and BPHYT_RS03415 are separated by 211 nucleotides BPHYT_RS03415 and BPHYT_RS03420 are separated by 118 nucleotides
BPHYT_RS03405: BPHYT_RS03405 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 767,829 to 768,887
_RS03405
BPHYT_RS03410: BPHYT_RS03410 - queuine tRNA-ribosyltransferase, at 769,050 to 770,264
_RS03410
BPHYT_RS03415: BPHYT_RS03415 - preprotein translocase subunit YajC, at 770,476 to 770,805
_RS03415
BPHYT_RS03420: BPHYT_RS03420 - preprotein translocase subunit SecD, at 770,924 to 772,987
_RS03420
Position (kb) 769
770
771 Strain fitness (log2 ratio) -1
0
1
2 at 768.288 kb on + strand, within BPHYT_RS03405 at 768.523 kb on - strand, within BPHYT_RS03405 at 768.691 kb on + strand, within BPHYT_RS03405 at 768.810 kb on + strand at 768.811 kb on - strand at 768.811 kb on - strand at 768.849 kb on + strand at 768.849 kb on + strand at 768.905 kb on + strand at 768.959 kb on + strand at 768.960 kb on - strand at 769.586 kb on - strand, within BPHYT_RS03410 at 770.082 kb on + strand, within BPHYT_RS03410 at 770.104 kb on + strand, within BPHYT_RS03410 at 770.151 kb on + strand at 770.151 kb on + strand at 770.151 kb on + strand at 770.155 kb on - strand at 770.155 kb on - strand at 770.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 768,288 + BPHYT_RS03405 0.43 +0.5 768,523 - BPHYT_RS03405 0.66 +1.5 768,691 + BPHYT_RS03405 0.81 +2.7 768,810 + +0.7 768,811 - -0.5 768,811 - -0.9 768,849 + +0.6 768,849 + -1.4 768,905 + -0.3 768,959 + -0.5 768,960 - -0.5 769,586 - BPHYT_RS03410 0.44 -0.4 770,082 + BPHYT_RS03410 0.85 -0.3 770,104 + BPHYT_RS03410 0.87 -0.1 770,151 + -0.2 770,151 + -0.5 770,151 + -0.1 770,155 - -0.0 770,155 - -1.1 770,155 - -0.4
Or see this region's nucleotide sequence