Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02250 and BPHYT_RS02255 are separated by 76 nucleotides BPHYT_RS02255 and BPHYT_RS02260 are separated by 3 nucleotides BPHYT_RS02260 and BPHYT_RS02265 are separated by 243 nucleotides
BPHYT_RS02250: BPHYT_RS02250 - Zn-dependent hydrolase, at 516,674 to 517,747
_RS02250
BPHYT_RS02255: BPHYT_RS02255 - hypothetical protein, at 517,824 to 518,288
_RS02255
BPHYT_RS02260: BPHYT_RS02260 - hypothetical protein, at 518,292 to 518,660
_RS02260
BPHYT_RS02265: BPHYT_RS02265 - peptide ABC transporter substrate-binding protein, at 518,904 to 520,490
_RS02265
Position (kb) 517
518
519 Strain fitness (log2 ratio) -1
0
1
2 at 517.215 kb on - strand, within BPHYT_RS02250 at 517.416 kb on + strand, within BPHYT_RS02250 at 517.468 kb on - strand, within BPHYT_RS02250 at 517.594 kb on + strand, within BPHYT_RS02250 at 517.822 kb on + strand at 517.825 kb on + strand at 517.825 kb on + strand at 517.826 kb on - strand at 518.003 kb on - strand, within BPHYT_RS02255 at 518.054 kb on + strand, within BPHYT_RS02255 at 518.056 kb on + strand, within BPHYT_RS02255 at 518.057 kb on - strand, within BPHYT_RS02255 at 518.091 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.102 kb on - strand, within BPHYT_RS02255 at 518.162 kb on + strand at 518.162 kb on + strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.265 kb on - strand at 518.359 kb on + strand, within BPHYT_RS02260 at 518.360 kb on - strand, within BPHYT_RS02260 at 518.399 kb on - strand, within BPHYT_RS02260 at 518.443 kb on - strand, within BPHYT_RS02260 at 518.479 kb on - strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.515 kb on + strand, within BPHYT_RS02260 at 518.531 kb on - strand, within BPHYT_RS02260 at 518.646 kb on - strand at 519.185 kb on - strand, within BPHYT_RS02265
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 517,215 - BPHYT_RS02250 0.50 +0.1 517,416 + BPHYT_RS02250 0.69 -0.1 517,468 - BPHYT_RS02250 0.74 +0.7 517,594 + BPHYT_RS02250 0.86 +0.6 517,822 + +0.1 517,825 + +0.5 517,825 + -0.3 517,826 - +0.5 518,003 - BPHYT_RS02255 0.38 +0.5 518,054 + BPHYT_RS02255 0.49 +0.0 518,056 + BPHYT_RS02255 0.50 -0.4 518,057 - BPHYT_RS02255 0.50 -1.4 518,091 + BPHYT_RS02255 0.57 +1.1 518,101 + BPHYT_RS02255 0.60 -1.0 518,101 + BPHYT_RS02255 0.60 -0.7 518,102 - BPHYT_RS02255 0.60 -0.9 518,162 + +2.0 518,162 + BPHYT_RS02255 0.73 -1.4 518,165 - BPHYT_RS02255 0.73 +0.9 518,165 - BPHYT_RS02255 0.73 +0.9 518,265 - +0.2 518,359 + BPHYT_RS02260 0.18 -0.8 518,360 - BPHYT_RS02260 0.18 +0.5 518,399 - BPHYT_RS02260 0.29 +0.4 518,443 - BPHYT_RS02260 0.41 +0.5 518,479 - BPHYT_RS02260 0.51 -1.1 518,485 + BPHYT_RS02260 0.52 +0.3 518,485 + BPHYT_RS02260 0.52 -0.2 518,515 + BPHYT_RS02260 0.60 -0.6 518,531 - BPHYT_RS02260 0.65 -0.5 518,646 - +1.0 519,185 - BPHYT_RS02265 0.18 +0.5
Or see this region's nucleotide sequence