Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS00275

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS00265 and BPHYT_RS00270 overlap by 4 nucleotidesBPHYT_RS00270 and BPHYT_RS00275 are separated by 37 nucleotidesBPHYT_RS00275 and BPHYT_RS00280 are separated by 2 nucleotides BPHYT_RS00265: BPHYT_RS00265 - branched-chain amino acid ABC transporter substrate-binding protein, at 67,674 to 69,488 _RS00265 BPHYT_RS00270: BPHYT_RS00270 - branched-chain amino acid ABC transporter, at 69,485 to 70,186 _RS00270 BPHYT_RS00275: BPHYT_RS00275 - urease subunit beta, at 70,224 to 70,910 _RS00275 BPHYT_RS00280: BPHYT_RS00280 - urease subunit alpha, at 70,913 to 72,628 _RS00280 Position (kb) 70 71Strain fitness (log2 ratio) -1 0 1 2at 69.286 kb on + strand, within BPHYT_RS00265at 69.315 kb on - strandat 69.315 kb on - strandat 69.453 kb on + strandat 69.453 kb on + strandat 69.454 kb on - strandat 69.455 kb on + strandat 69.456 kb on - strandat 69.525 kb on - strandat 69.732 kb on - strand, within BPHYT_RS00270at 69.732 kb on - strand, within BPHYT_RS00270at 70.094 kb on + strand, within BPHYT_RS00270at 70.095 kb on - strand, within BPHYT_RS00270at 70.306 kb on + strand, within BPHYT_RS00275at 70.307 kb on - strand, within BPHYT_RS00275at 70.460 kb on - strand, within BPHYT_RS00275at 70.460 kb on - strand, within BPHYT_RS00275at 70.460 kb on - strand, within BPHYT_RS00275at 70.607 kb on + strand, within BPHYT_RS00275at 70.608 kb on - strand, within BPHYT_RS00275at 70.683 kb on - strand, within BPHYT_RS00275at 70.772 kb on + strand, within BPHYT_RS00275at 70.772 kb on + strand, within BPHYT_RS00275at 70.772 kb on + strand, within BPHYT_RS00275at 70.773 kb on - strand, within BPHYT_RS00275at 70.908 kb on + strandat 70.908 kb on + strandat 70.909 kb on - strandat 70.909 kb on - strandat 70.909 kb on - strandat 70.909 kb on - strandat 70.941 kb on - strandat 71.200 kb on + strand, within BPHYT_RS00280at 71.200 kb on + strand, within BPHYT_RS00280at 71.733 kb on - strand, within BPHYT_RS00280at 71.733 kb on - strand, within BPHYT_RS00280at 71.842 kb on + strand, within BPHYT_RS00280at 71.842 kb on + strand, within BPHYT_RS00280at 71.843 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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69,286 + BPHYT_RS00265 0.89 -0.9
69,315 - +0.9
69,315 - -0.7
69,453 + +0.1
69,453 + +0.1
69,454 - +0.7
69,455 + -1.1
69,456 - +2.2
69,525 - +0.6
69,732 - BPHYT_RS00270 0.35 -0.7
69,732 - BPHYT_RS00270 0.35 -0.8
70,094 + BPHYT_RS00270 0.87 +0.3
70,095 - BPHYT_RS00270 0.87 -0.7
70,306 + BPHYT_RS00275 0.12 -0.6
70,307 - BPHYT_RS00275 0.12 -0.3
70,460 - BPHYT_RS00275 0.34 +0.1
70,460 - BPHYT_RS00275 0.34 -0.4
70,460 - BPHYT_RS00275 0.34 -0.2
70,607 + BPHYT_RS00275 0.56 +0.6
70,608 - BPHYT_RS00275 0.56 -0.3
70,683 - BPHYT_RS00275 0.67 +1.1
70,772 + BPHYT_RS00275 0.80 +0.3
70,772 + BPHYT_RS00275 0.80 +0.3
70,772 + BPHYT_RS00275 0.80 -0.4
70,773 - BPHYT_RS00275 0.80 +1.0
70,908 + -0.2
70,908 + +0.2
70,909 - -0.1
70,909 - +1.3
70,909 - +0.9
70,909 - +1.0
70,941 - +0.1
71,200 + BPHYT_RS00280 0.17 -0.4
71,200 + BPHYT_RS00280 0.17 -0.0
71,733 - BPHYT_RS00280 0.48 -0.9
71,733 - BPHYT_RS00280 0.48 +1.5
71,842 + BPHYT_RS00280 0.54 -1.5
71,842 + BPHYT_RS00280 0.54 -0.6
71,843 - +2.3

Or see this region's nucleotide sequence