Experiment: CDM1 without Uracil
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16140 and BBR_RS16145 are separated by 75 nucleotides BBR_RS16145 and BBR_RS16150 are separated by 145 nucleotides BBR_RS16150 and BBR_RS16155 are separated by 128 nucleotides
BBR_RS16140: BBR_RS16140 - long-chain fatty acid--CoA ligase, at 1,368,687 to 1,370,747
_RS16140
BBR_RS16145: BBR_RS16145 - hypothetical protein, at 1,370,823 to 1,371,311
_RS16145
BBR_RS16150: BBR_RS16150 - GuaB3 family IMP dehydrogenase-related protein, at 1,371,457 to 1,372,581
_RS16150
BBR_RS16155: BBR_RS16155 - isocitrate dehydrogenase (NADP(+)), at 1,372,710 to 1,373,930
_RS16155
Position (kb)
1371
1372
1373 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1370.526 kb on - strand, within BBR_RS16140 at 1370.538 kb on - strand, within BBR_RS16140 at 1370.538 kb on - strand, within BBR_RS16140 at 1370.553 kb on + strand at 1370.558 kb on + strand at 1370.560 kb on + strand at 1370.560 kb on + strand at 1370.568 kb on - strand at 1370.581 kb on + strand at 1370.613 kb on + strand at 1370.666 kb on + strand at 1370.738 kb on + strand at 1370.771 kb on + strand at 1370.771 kb on + strand at 1370.780 kb on - strand at 1370.805 kb on - strand at 1370.821 kb on + strand at 1370.842 kb on + strand at 1370.842 kb on + strand at 1370.850 kb on - strand at 1370.850 kb on - strand at 1370.908 kb on - strand, within BBR_RS16145 at 1370.916 kb on - strand, within BBR_RS16145 at 1370.930 kb on - strand, within BBR_RS16145 at 1370.988 kb on + strand, within BBR_RS16145 at 1371.017 kb on + strand, within BBR_RS16145 at 1371.028 kb on - strand, within BBR_RS16145 at 1371.036 kb on + strand, within BBR_RS16145 at 1371.044 kb on - strand, within BBR_RS16145 at 1371.046 kb on + strand, within BBR_RS16145 at 1371.054 kb on - strand, within BBR_RS16145 at 1371.064 kb on - strand, within BBR_RS16145 at 1371.067 kb on + strand, within BBR_RS16145 at 1371.237 kb on + strand, within BBR_RS16145 at 1371.263 kb on - strand at 1371.263 kb on - strand at 1371.279 kb on - strand at 1371.360 kb on + strand at 1371.368 kb on - strand at 1371.409 kb on + strand at 1371.458 kb on - strand at 1371.609 kb on - strand, within BBR_RS16150 at 1371.655 kb on - strand, within BBR_RS16150 at 1371.660 kb on - strand, within BBR_RS16150 at 1371.684 kb on - strand, within BBR_RS16150 at 1371.700 kb on - strand, within BBR_RS16150 at 1371.700 kb on - strand, within BBR_RS16150 at 1371.804 kb on + strand, within BBR_RS16150 at 1371.805 kb on - strand, within BBR_RS16150 at 1371.812 kb on - strand, within BBR_RS16150 at 1371.815 kb on - strand, within BBR_RS16150 at 1371.866 kb on - strand, within BBR_RS16150 at 1371.882 kb on - strand, within BBR_RS16150 at 1371.893 kb on - strand, within BBR_RS16150 at 1371.970 kb on + strand, within BBR_RS16150 at 1371.983 kb on + strand, within BBR_RS16150 at 1371.993 kb on - strand, within BBR_RS16150 at 1371.993 kb on - strand, within BBR_RS16150 at 1372.025 kb on - strand, within BBR_RS16150 at 1372.064 kb on + strand, within BBR_RS16150 at 1372.092 kb on - strand, within BBR_RS16150 at 1372.094 kb on - strand, within BBR_RS16150 at 1372.144 kb on - strand, within BBR_RS16150 at 1372.144 kb on - strand, within BBR_RS16150 at 1372.179 kb on + strand, within BBR_RS16150 at 1372.224 kb on + strand, within BBR_RS16150 at 1372.224 kb on + strand, within BBR_RS16150 at 1372.257 kb on + strand, within BBR_RS16150 at 1372.338 kb on + strand, within BBR_RS16150 at 1372.600 kb on - strand at 1372.618 kb on - strand at 1372.622 kb on + strand at 1372.672 kb on + strand at 1372.680 kb on - strand at 1372.701 kb on + strand at 1372.748 kb on - strand at 1372.932 kb on + strand, within BBR_RS16155 at 1373.031 kb on - strand, within BBR_RS16155 at 1373.059 kb on - strand, within BBR_RS16155 at 1373.059 kb on - strand, within BBR_RS16155 at 1373.087 kb on + strand, within BBR_RS16155 at 1373.123 kb on + strand, within BBR_RS16155 at 1373.123 kb on + strand, within BBR_RS16155 at 1373.123 kb on + strand, within BBR_RS16155 at 1373.131 kb on - strand, within BBR_RS16155 at 1373.131 kb on - strand, within BBR_RS16155 at 1373.131 kb on - strand, within BBR_RS16155 at 1373.131 kb on - strand, within BBR_RS16155 at 1373.162 kb on + strand, within BBR_RS16155 at 1373.174 kb on - strand, within BBR_RS16155 at 1373.222 kb on - strand, within BBR_RS16155 at 1373.222 kb on - strand, within BBR_RS16155 at 1373.226 kb on + strand, within BBR_RS16155 at 1373.258 kb on - strand, within BBR_RS16155 at 1373.318 kb on + strand, within BBR_RS16155 at 1373.399 kb on + strand, within BBR_RS16155 at 1373.405 kb on - strand, within BBR_RS16155 at 1373.417 kb on - strand, within BBR_RS16155 at 1373.443 kb on + strand, within BBR_RS16155 at 1373.453 kb on - strand, within BBR_RS16155 at 1373.472 kb on + strand, within BBR_RS16155 at 1373.513 kb on - strand, within BBR_RS16155 at 1373.543 kb on - strand, within BBR_RS16155 at 1373.548 kb on - strand, within BBR_RS16155
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Uracil remove 1,370,526 - BBR_RS16140 0.89 +1.1 1,370,538 - BBR_RS16140 0.90 +0.4 1,370,538 - BBR_RS16140 0.90 +0.6 1,370,553 + -0.1 1,370,558 + -1.9 1,370,560 + +1.1 1,370,560 + +0.7 1,370,568 - +0.9 1,370,581 + +1.1 1,370,613 + +0.6 1,370,666 + -0.4 1,370,738 + +0.3 1,370,771 + +2.3 1,370,771 + -0.3 1,370,780 - -1.9 1,370,805 - +1.1 1,370,821 + -0.2 1,370,842 + -0.8 1,370,842 + +0.1 1,370,850 - -0.3 1,370,850 - -2.2 1,370,908 - BBR_RS16145 0.17 +1.2 1,370,916 - BBR_RS16145 0.19 +1.5 1,370,930 - BBR_RS16145 0.22 -1.2 1,370,988 + BBR_RS16145 0.34 -1.6 1,371,017 + BBR_RS16145 0.40 -2.3 1,371,028 - BBR_RS16145 0.42 +0.0 1,371,036 + BBR_RS16145 0.44 -2.3 1,371,044 - BBR_RS16145 0.45 -0.4 1,371,046 + BBR_RS16145 0.46 -2.0 1,371,054 - BBR_RS16145 0.47 -0.6 1,371,064 - BBR_RS16145 0.49 -1.2 1,371,067 + BBR_RS16145 0.50 -2.2 1,371,237 + BBR_RS16145 0.85 -0.5 1,371,263 - +1.7 1,371,263 - -1.1 1,371,279 - +0.6 1,371,360 + -1.7 1,371,368 - +0.1 1,371,409 + -3.1 1,371,458 - -0.7 1,371,609 - BBR_RS16150 0.14 -1.6 1,371,655 - BBR_RS16150 0.18 +0.1 1,371,660 - BBR_RS16150 0.18 -2.4 1,371,684 - BBR_RS16150 0.20 -1.8 1,371,700 - BBR_RS16150 0.22 +1.6 1,371,700 - BBR_RS16150 0.22 -0.6 1,371,804 + BBR_RS16150 0.31 -1.4 1,371,805 - BBR_RS16150 0.31 -0.0 1,371,812 - BBR_RS16150 0.32 -2.8 1,371,815 - BBR_RS16150 0.32 -1.1 1,371,866 - BBR_RS16150 0.36 -1.9 1,371,882 - BBR_RS16150 0.38 -3.3 1,371,893 - BBR_RS16150 0.39 -2.2 1,371,970 + BBR_RS16150 0.46 -2.5 1,371,983 + BBR_RS16150 0.47 -1.6 1,371,993 - BBR_RS16150 0.48 +0.7 1,371,993 - BBR_RS16150 0.48 -1.4 1,372,025 - BBR_RS16150 0.50 -2.2 1,372,064 + BBR_RS16150 0.54 -2.4 1,372,092 - BBR_RS16150 0.56 -2.8 1,372,094 - BBR_RS16150 0.57 -2.9 1,372,144 - BBR_RS16150 0.61 -2.5 1,372,144 - BBR_RS16150 0.61 -2.9 1,372,179 + BBR_RS16150 0.64 -2.2 1,372,224 + BBR_RS16150 0.68 -2.7 1,372,224 + BBR_RS16150 0.68 -3.2 1,372,257 + BBR_RS16150 0.71 -1.5 1,372,338 + BBR_RS16150 0.78 -3.3 1,372,600 - -1.3 1,372,618 - -2.1 1,372,622 + -1.7 1,372,672 + -1.1 1,372,680 - -3.8 1,372,701 + -1.4 1,372,748 - -2.4 1,372,932 + BBR_RS16155 0.18 -1.8 1,373,031 - BBR_RS16155 0.26 -2.5 1,373,059 - BBR_RS16155 0.29 -2.1 1,373,059 - BBR_RS16155 0.29 -4.1 1,373,087 + BBR_RS16155 0.31 -2.4 1,373,123 + BBR_RS16155 0.34 +0.2 1,373,123 + BBR_RS16155 0.34 -0.7 1,373,123 + BBR_RS16155 0.34 -1.7 1,373,131 - BBR_RS16155 0.34 -0.2 1,373,131 - BBR_RS16155 0.34 -3.5 1,373,131 - BBR_RS16155 0.34 -2.0 1,373,131 - BBR_RS16155 0.34 -2.2 1,373,162 + BBR_RS16155 0.37 -1.7 1,373,174 - BBR_RS16155 0.38 -1.8 1,373,222 - BBR_RS16155 0.42 -4.4 1,373,222 - BBR_RS16155 0.42 -2.8 1,373,226 + BBR_RS16155 0.42 -2.6 1,373,258 - BBR_RS16155 0.45 -0.4 1,373,318 + BBR_RS16155 0.50 -3.7 1,373,399 + BBR_RS16155 0.56 -3.6 1,373,405 - BBR_RS16155 0.57 -4.9 1,373,417 - BBR_RS16155 0.58 -3.8 1,373,443 + BBR_RS16155 0.60 -2.6 1,373,453 - BBR_RS16155 0.61 -3.4 1,373,472 + BBR_RS16155 0.62 -2.1 1,373,513 - BBR_RS16155 0.66 -1.1 1,373,543 - BBR_RS16155 0.68 -1.9 1,373,548 - BBR_RS16155 0.69 -2.1
Or see this region's nucleotide sequence