Experiment: CDM1 without Uracil
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14350 and BBR_RS14355 are separated by 225 nucleotides BBR_RS14355 and BBR_RS14360 are separated by 316 nucleotides BBR_RS14360 and BBR_RS20825 overlap by 29 nucleotides BBR_RS20825 and BBR_RS14365 overlap by 2 nucleotides
BBR_RS14350: BBR_RS14350 - hypothetical protein, at 994,662 to 994,889
_RS14350
BBR_RS14355: BBR_RS14355 - tRNA-Pro, at 995,115 to 995,191
_RS14355
BBR_RS14360: BBR_RS14360 - hypothetical protein, at 995,508 to 996,182
_RS14360
BBR_RS20825: BBR_RS20825 - hypothetical protein, at 996,154 to 996,345
_RS20825
BBR_RS14365: BBR_RS14365 - hypothetical protein, at 996,344 to 997,516
_RS14365
Position (kb) 995
996
997 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 994.510 kb on - strand at 994.606 kb on + strand at 994.614 kb on - strand at 994.614 kb on - strand at 994.702 kb on - strand, within BBR_RS14350 at 994.724 kb on - strand, within BBR_RS14350 at 994.729 kb on + strand, within BBR_RS14350 at 994.747 kb on - strand, within BBR_RS14350 at 994.770 kb on + strand, within BBR_RS14350 at 994.794 kb on + strand, within BBR_RS14350 at 994.881 kb on + strand at 994.889 kb on - strand at 995.015 kb on + strand at 995.015 kb on + strand at 995.375 kb on - strand at 995.439 kb on + strand at 995.484 kb on + strand at 995.492 kb on + strand at 995.556 kb on - strand at 995.558 kb on + strand at 995.599 kb on - strand, within BBR_RS14360 at 995.654 kb on - strand, within BBR_RS14360 at 995.764 kb on + strand, within BBR_RS14360 at 995.764 kb on + strand, within BBR_RS14360 at 995.801 kb on + strand, within BBR_RS14360 at 995.846 kb on + strand, within BBR_RS14360 at 995.854 kb on - strand, within BBR_RS14360 at 995.854 kb on - strand, within BBR_RS14360 at 995.890 kb on + strand, within BBR_RS14360 at 995.981 kb on + strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 995.989 kb on - strand, within BBR_RS14360 at 996.002 kb on - strand, within BBR_RS14360 at 996.014 kb on + strand, within BBR_RS14360 at 996.023 kb on - strand, within BBR_RS14360 at 996.040 kb on + strand, within BBR_RS14360 at 996.045 kb on + strand, within BBR_RS14360 at 996.066 kb on - strand, within BBR_RS14360 at 996.080 kb on - strand, within BBR_RS14360 at 996.143 kb on - strand at 996.244 kb on + strand, within BBR_RS20825 at 996.244 kb on + strand, within BBR_RS20825 at 996.344 kb on - strand at 996.432 kb on - strand at 996.481 kb on + strand, within BBR_RS14365 at 996.491 kb on - strand, within BBR_RS14365 at 996.507 kb on + strand, within BBR_RS14365 at 996.527 kb on + strand, within BBR_RS14365 at 996.565 kb on + strand, within BBR_RS14365 at 996.578 kb on - strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.730 kb on - strand, within BBR_RS14365 at 996.742 kb on + strand, within BBR_RS14365 at 996.833 kb on + strand, within BBR_RS14365 at 996.848 kb on + strand, within BBR_RS14365 at 996.928 kb on + strand, within BBR_RS14365 at 996.932 kb on - strand, within BBR_RS14365 at 997.011 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.029 kb on - strand, within BBR_RS14365 at 997.143 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Uracil remove 994,510 - -0.8 994,606 + +0.3 994,614 - -2.7 994,614 - +0.4 994,702 - BBR_RS14350 0.18 +0.6 994,724 - BBR_RS14350 0.27 -0.2 994,729 + BBR_RS14350 0.29 +0.3 994,747 - BBR_RS14350 0.37 +0.5 994,770 + BBR_RS14350 0.47 -0.1 994,794 + BBR_RS14350 0.58 +0.4 994,881 + -1.8 994,889 - +0.4 995,015 + -3.1 995,015 + -1.3 995,375 - -3.2 995,439 + +0.8 995,484 + +1.3 995,492 + -0.9 995,556 - +0.5 995,558 + +1.6 995,599 - BBR_RS14360 0.13 -1.6 995,654 - BBR_RS14360 0.22 -1.2 995,764 + BBR_RS14360 0.38 -0.9 995,764 + BBR_RS14360 0.38 -1.2 995,801 + BBR_RS14360 0.43 -1.9 995,846 + BBR_RS14360 0.50 -0.4 995,854 - BBR_RS14360 0.51 -0.5 995,854 - BBR_RS14360 0.51 -0.1 995,890 + BBR_RS14360 0.57 +0.3 995,981 + BBR_RS14360 0.70 -2.4 995,989 - BBR_RS14360 0.71 -1.8 995,989 - BBR_RS14360 0.71 +0.2 995,989 - BBR_RS14360 0.71 -0.5 995,989 - BBR_RS14360 0.71 -0.3 995,989 - BBR_RS14360 0.71 +0.0 995,989 - BBR_RS14360 0.71 +0.6 996,002 - BBR_RS14360 0.73 +0.9 996,014 + BBR_RS14360 0.75 -1.9 996,023 - BBR_RS14360 0.76 -1.9 996,040 + BBR_RS14360 0.79 -1.2 996,045 + BBR_RS14360 0.80 -2.1 996,066 - BBR_RS14360 0.83 +0.3 996,080 - BBR_RS14360 0.85 -2.3 996,143 - -3.2 996,244 + BBR_RS20825 0.47 -2.0 996,244 + BBR_RS20825 0.47 -0.1 996,344 - -2.4 996,432 - -2.4 996,481 + BBR_RS14365 0.12 -3.1 996,491 - BBR_RS14365 0.13 -1.8 996,507 + BBR_RS14365 0.14 -0.0 996,527 + BBR_RS14365 0.16 -1.0 996,565 + BBR_RS14365 0.19 -3.8 996,578 - BBR_RS14365 0.20 -2.7 996,616 + BBR_RS14365 0.23 -3.7 996,616 + BBR_RS14365 0.23 -2.2 996,730 - BBR_RS14365 0.33 -3.5 996,742 + BBR_RS14365 0.34 -4.1 996,833 + BBR_RS14365 0.42 -2.5 996,848 + BBR_RS14365 0.43 -3.7 996,928 + BBR_RS14365 0.50 -0.7 996,932 - BBR_RS14365 0.50 -1.5 997,011 + BBR_RS14365 0.57 -1.6 997,021 + BBR_RS14365 0.58 +1.0 997,021 + BBR_RS14365 0.58 -3.3 997,029 - BBR_RS14365 0.58 -2.7 997,143 - BBR_RS14365 0.68 -1.6 997,150 - BBR_RS14365 0.69 -0.9 997,150 - BBR_RS14365 0.69 -2.8
Or see this region's nucleotide sequence