Experiment: CDM1 without Uracil
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14260 and BBR_RS14265 are separated by 157 nucleotides BBR_RS14265 and BBR_RS14270 are separated by 104 nucleotides
BBR_RS14260: BBR_RS14260 - ABC transporter ATP-binding protein, at 973,574 to 974,446
_RS14260
BBR_RS14265: BBR_RS14265 - Solute-binding protein of ABC transporter system for metals, at 974,604 to 975,776
_RS14265
BBR_RS14270: BBR_RS14270 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 975,881 to 976,759
_RS14270
Position (kb)
974
975
976 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 973.727 kb on - strand, within BBR_RS14260 at 973.761 kb on - strand, within BBR_RS14260 at 974.456 kb on - strand at 974.523 kb on - strand at 974.562 kb on + strand at 974.584 kb on - strand at 974.608 kb on - strand at 974.628 kb on - strand at 974.646 kb on - strand at 974.648 kb on + strand at 974.659 kb on + strand at 974.662 kb on - strand at 974.662 kb on - strand at 974.682 kb on - strand at 974.738 kb on - strand, within BBR_RS14265 at 974.776 kb on - strand, within BBR_RS14265 at 974.834 kb on + strand, within BBR_RS14265 at 974.841 kb on + strand, within BBR_RS14265 at 974.860 kb on - strand, within BBR_RS14265 at 974.893 kb on - strand, within BBR_RS14265 at 974.903 kb on + strand, within BBR_RS14265 at 974.915 kb on - strand, within BBR_RS14265 at 974.926 kb on + strand, within BBR_RS14265 at 975.051 kb on + strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.113 kb on - strand, within BBR_RS14265 at 975.151 kb on + strand, within BBR_RS14265 at 975.159 kb on + strand, within BBR_RS14265 at 975.191 kb on - strand, within BBR_RS14265 at 975.288 kb on + strand, within BBR_RS14265 at 975.295 kb on + strand, within BBR_RS14265 at 975.296 kb on - strand, within BBR_RS14265 at 975.324 kb on + strand, within BBR_RS14265 at 975.469 kb on + strand, within BBR_RS14265 at 975.486 kb on + strand, within BBR_RS14265 at 975.489 kb on - strand, within BBR_RS14265 at 975.489 kb on - strand, within BBR_RS14265 at 975.516 kb on - strand, within BBR_RS14265 at 975.611 kb on + strand, within BBR_RS14265 at 975.674 kb on - strand at 975.716 kb on + strand at 975.818 kb on + strand at 975.860 kb on + strand at 975.860 kb on + strand at 975.875 kb on + strand at 975.875 kb on + strand at 975.875 kb on + strand at 976.749 kb on + strand at 976.757 kb on - strand at 976.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Uracil remove 973,727 - BBR_RS14260 0.18 -2.4 973,761 - BBR_RS14260 0.21 -2.2 974,456 - +0.6 974,523 - -2.0 974,562 + -2.2 974,584 - +0.5 974,608 - +2.5 974,628 - +1.3 974,646 - -0.1 974,648 + -1.7 974,659 + -0.9 974,662 - +0.1 974,662 - +0.7 974,682 - +0.8 974,738 - BBR_RS14265 0.11 -2.9 974,776 - BBR_RS14265 0.15 -2.8 974,834 + BBR_RS14265 0.20 -2.9 974,841 + BBR_RS14265 0.20 -3.5 974,860 - BBR_RS14265 0.22 -2.4 974,893 - BBR_RS14265 0.25 -1.5 974,903 + BBR_RS14265 0.25 -2.7 974,915 - BBR_RS14265 0.27 -2.6 974,926 + BBR_RS14265 0.27 -3.4 975,051 + BBR_RS14265 0.38 -3.0 975,102 - BBR_RS14265 0.42 -2.8 975,102 - BBR_RS14265 0.42 +0.0 975,113 - BBR_RS14265 0.43 -2.7 975,151 + BBR_RS14265 0.47 -0.5 975,159 + BBR_RS14265 0.47 -3.0 975,191 - BBR_RS14265 0.50 -3.7 975,288 + BBR_RS14265 0.58 -1.4 975,295 + BBR_RS14265 0.59 -0.0 975,296 - BBR_RS14265 0.59 -2.5 975,324 + BBR_RS14265 0.61 -2.8 975,469 + BBR_RS14265 0.74 -4.7 975,486 + BBR_RS14265 0.75 -3.8 975,489 - BBR_RS14265 0.75 -2.7 975,489 - BBR_RS14265 0.75 -2.7 975,516 - BBR_RS14265 0.78 -2.2 975,611 + BBR_RS14265 0.86 -3.0 975,674 - -2.4 975,716 + -1.3 975,818 + -1.6 975,860 + +0.1 975,860 + +0.3 975,875 + -0.7 975,875 + +1.0 975,875 + +0.8 976,749 + +0.5 976,757 - +0.4 976,766 + +0.7
Or see this region's nucleotide sequence