Experiment: CDM1 without Uracil
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11585 and BBR_RS11590 are separated by 57 nucleotides BBR_RS11590 and BBR_RS11595 are separated by 19 nucleotides BBR_RS11595 and BBR_RS11600 are separated by 228 nucleotides BBR_RS11600 and BBR_RS20630 are separated by 229 nucleotides BBR_RS20630 and BBR_RS11605 are separated by 47 nucleotides
BBR_RS11585: BBR_RS11585 - single-stranded DNA-binding protein, at 357,922 to 358,566
_RS11585
BBR_RS11590: BBR_RS11590 - 30S ribosomal protein S18, at 358,624 to 358,872
_RS11590
BBR_RS11595: BBR_RS11595 - 50S ribosomal protein L9, at 358,892 to 359,338
_RS11595
BBR_RS11600: BBR_RS11600 - tRNA-Lys, at 359,567 to 359,640
_RS11600
BBR_RS20630: BBR_RS20630 - transposase, at 359,870 to 360,107
_RS20630
BBR_RS11605: BBR_RS11605 - hypothetical protein, at 360,155 to 360,847
_RS11605
Position (kb) 358
359
360 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 358.841 kb on + strand, within BBR_RS11590 at 358.882 kb on + strand at 358.932 kb on + strand at 358.932 kb on + strand at 358.932 kb on + strand at 358.932 kb on + strand at 358.936 kb on + strand at 358.963 kb on + strand, within BBR_RS11595 at 358.999 kb on + strand, within BBR_RS11595 at 359.017 kb on + strand, within BBR_RS11595 at 359.017 kb on + strand, within BBR_RS11595 at 359.017 kb on + strand, within BBR_RS11595 at 359.045 kb on + strand, within BBR_RS11595 at 359.060 kb on + strand, within BBR_RS11595 at 359.112 kb on + strand, within BBR_RS11595 at 359.126 kb on + strand, within BBR_RS11595 at 359.139 kb on + strand, within BBR_RS11595 at 359.196 kb on + strand, within BBR_RS11595 at 359.216 kb on + strand, within BBR_RS11595 at 359.216 kb on + strand, within BBR_RS11595 at 359.219 kb on + strand, within BBR_RS11595 at 359.251 kb on + strand, within BBR_RS11595 at 359.342 kb on + strand at 359.355 kb on + strand at 359.381 kb on + strand at 359.389 kb on + strand at 359.389 kb on + strand at 359.389 kb on + strand at 359.412 kb on + strand at 359.643 kb on + strand at 359.719 kb on - strand at 359.754 kb on + strand at 359.847 kb on + strand at 359.847 kb on + strand at 359.853 kb on + strand at 359.853 kb on + strand at 359.855 kb on - strand at 359.861 kb on - strand at 359.897 kb on - strand, within BBR_RS20630 at 359.897 kb on - strand, within BBR_RS20630 at 359.901 kb on + strand, within BBR_RS20630 at 359.972 kb on + strand, within BBR_RS20630 at 359.979 kb on - strand, within BBR_RS20630 at 360.000 kb on - strand, within BBR_RS20630 at 360.000 kb on - strand, within BBR_RS20630 at 360.014 kb on + strand, within BBR_RS20630 at 360.015 kb on + strand, within BBR_RS20630 at 360.015 kb on + strand, within BBR_RS20630 at 360.049 kb on - strand, within BBR_RS20630 at 360.061 kb on - strand, within BBR_RS20630 at 360.093 kb on + strand at 360.093 kb on + strand at 360.120 kb on + strand at 360.151 kb on - strand at 360.153 kb on + strand at 360.161 kb on - strand at 360.258 kb on - strand, within BBR_RS11605 at 360.268 kb on - strand, within BBR_RS11605 at 360.334 kb on + strand, within BBR_RS11605
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Uracil remove 358,841 + BBR_RS11590 0.87 -0.2 358,882 + -1.0 358,932 + -3.0 358,932 + -3.3 358,932 + -2.3 358,932 + -1.8 358,936 + -3.1 358,963 + BBR_RS11595 0.16 -1.6 358,999 + BBR_RS11595 0.24 -3.9 359,017 + BBR_RS11595 0.28 -0.4 359,017 + BBR_RS11595 0.28 -3.2 359,017 + BBR_RS11595 0.28 -3.1 359,045 + BBR_RS11595 0.34 -3.0 359,060 + BBR_RS11595 0.38 -3.5 359,112 + BBR_RS11595 0.49 -3.2 359,126 + BBR_RS11595 0.52 -2.3 359,139 + BBR_RS11595 0.55 -0.7 359,196 + BBR_RS11595 0.68 -2.5 359,216 + BBR_RS11595 0.72 -2.8 359,216 + BBR_RS11595 0.72 +0.0 359,219 + BBR_RS11595 0.73 +0.4 359,251 + BBR_RS11595 0.80 -3.5 359,342 + +0.8 359,355 + +1.7 359,381 + +0.9 359,389 + +1.2 359,389 + +0.3 359,389 + -0.3 359,412 + -0.1 359,643 + +1.2 359,719 - -0.1 359,754 + +0.5 359,847 + +0.3 359,847 + -0.2 359,853 + -0.5 359,853 + +2.2 359,855 - -0.1 359,861 - +0.8 359,897 - BBR_RS20630 0.11 +0.1 359,897 - BBR_RS20630 0.11 -2.6 359,901 + BBR_RS20630 0.13 +0.7 359,972 + BBR_RS20630 0.43 +0.8 359,979 - BBR_RS20630 0.46 +0.3 360,000 - BBR_RS20630 0.55 +0.2 360,000 - BBR_RS20630 0.55 +2.2 360,014 + BBR_RS20630 0.61 -0.8 360,015 + BBR_RS20630 0.61 -3.3 360,015 + BBR_RS20630 0.61 -2.1 360,049 - BBR_RS20630 0.75 -0.6 360,061 - BBR_RS20630 0.80 +0.5 360,093 + -1.3 360,093 + +1.0 360,120 + -0.9 360,151 - -0.5 360,153 + +0.1 360,161 - +0.1 360,258 - BBR_RS11605 0.15 +0.6 360,268 - BBR_RS11605 0.16 +0.5 360,334 + BBR_RS11605 0.26 -1.3
Or see this region's nucleotide sequence