Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27025 and RR42_RS27030 are separated by 436 nucleotides RR42_RS27030 and RR42_RS27035 are separated by 121 nucleotides RR42_RS27035 and RR42_RS27040 are separated by 3 nucleotides RR42_RS27040 and RR42_RS27045 are separated by 87 nucleotides
RR42_RS27025: RR42_RS27025 - hypothetical protein, at 1,416,658 to 1,417,389
_RS27025
RR42_RS27030: RR42_RS27030 - hypothetical protein, at 1,417,826 to 1,418,530
_RS27030
RR42_RS27035: RR42_RS27035 - hypothetical protein, at 1,418,652 to 1,418,831
_RS27035
RR42_RS27040: RR42_RS27040 - hypothetical protein, at 1,418,835 to 1,419,020
_RS27040
RR42_RS27045: RR42_RS27045 - hypothetical protein, at 1,419,108 to 1,419,506
_RS27045
Position (kb) 1417
1418
1419 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1416.962 kb on + strand, within RR42_RS27025 at 1416.963 kb on - strand, within RR42_RS27025 at 1416.963 kb on - strand, within RR42_RS27025 at 1416.963 kb on - strand, within RR42_RS27025 at 1417.084 kb on - strand, within RR42_RS27025 at 1417.256 kb on + strand, within RR42_RS27025 at 1417.256 kb on + strand, within RR42_RS27025 at 1417.256 kb on + strand, within RR42_RS27025 at 1417.256 kb on + strand, within RR42_RS27025 at 1417.256 kb on + strand, within RR42_RS27025 at 1417.256 kb on + strand, within RR42_RS27025 at 1417.257 kb on - strand, within RR42_RS27025 at 1417.257 kb on - strand, within RR42_RS27025 at 1417.257 kb on - strand, within RR42_RS27025 at 1417.257 kb on - strand, within RR42_RS27025 at 1417.368 kb on + strand at 1417.447 kb on - strand at 1417.456 kb on - strand at 1417.492 kb on + strand at 1417.492 kb on + strand at 1417.492 kb on + strand at 1417.492 kb on + strand at 1417.492 kb on + strand at 1417.492 kb on + strand at 1417.493 kb on - strand at 1417.493 kb on - strand at 1417.494 kb on + strand at 1417.962 kb on + strand, within RR42_RS27030 at 1417.963 kb on - strand, within RR42_RS27030 at 1417.966 kb on + strand, within RR42_RS27030 at 1417.967 kb on - strand, within RR42_RS27030 at 1417.967 kb on - strand, within RR42_RS27030 at 1418.136 kb on + strand, within RR42_RS27030 at 1418.137 kb on - strand, within RR42_RS27030 at 1418.137 kb on - strand, within RR42_RS27030 at 1418.259 kb on + strand, within RR42_RS27030 at 1418.425 kb on - strand, within RR42_RS27030 at 1418.616 kb on + strand at 1418.616 kb on + strand at 1418.617 kb on - strand at 1418.617 kb on - strand at 1418.621 kb on + strand at 1418.621 kb on + strand at 1418.744 kb on + strand, within RR42_RS27035 at 1418.745 kb on - strand, within RR42_RS27035 at 1418.751 kb on - strand, within RR42_RS27035 at 1418.751 kb on - strand, within RR42_RS27035 at 1419.317 kb on - strand, within RR42_RS27045 at 1419.376 kb on + strand, within RR42_RS27045 at 1419.417 kb on + strand, within RR42_RS27045
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,416,962 + RR42_RS27025 0.42 +1.5 1,416,963 - RR42_RS27025 0.42 -0.3 1,416,963 - RR42_RS27025 0.42 +0.7 1,416,963 - RR42_RS27025 0.42 -1.0 1,417,084 - RR42_RS27025 0.58 -0.6 1,417,256 + RR42_RS27025 0.82 -0.6 1,417,256 + RR42_RS27025 0.82 -0.3 1,417,256 + RR42_RS27025 0.82 -0.4 1,417,256 + RR42_RS27025 0.82 +0.9 1,417,256 + RR42_RS27025 0.82 +1.8 1,417,256 + RR42_RS27025 0.82 -0.2 1,417,257 - RR42_RS27025 0.82 +1.0 1,417,257 - RR42_RS27025 0.82 +1.4 1,417,257 - RR42_RS27025 0.82 +3.3 1,417,257 - RR42_RS27025 0.82 +0.0 1,417,368 + -0.3 1,417,447 - -0.0 1,417,456 - -0.2 1,417,492 + -0.7 1,417,492 + +0.1 1,417,492 + +0.1 1,417,492 + -0.4 1,417,492 + -2.6 1,417,492 + -0.1 1,417,493 - +1.0 1,417,493 - -0.4 1,417,494 + -1.4 1,417,962 + RR42_RS27030 0.19 +0.4 1,417,963 - RR42_RS27030 0.19 +0.0 1,417,966 + RR42_RS27030 0.20 +1.1 1,417,967 - RR42_RS27030 0.20 +1.1 1,417,967 - RR42_RS27030 0.20 -0.1 1,418,136 + RR42_RS27030 0.44 +0.4 1,418,137 - RR42_RS27030 0.44 +1.0 1,418,137 - RR42_RS27030 0.44 +0.9 1,418,259 + RR42_RS27030 0.61 -0.0 1,418,425 - RR42_RS27030 0.85 -0.4 1,418,616 + -0.1 1,418,616 + -0.8 1,418,617 - +1.2 1,418,617 - +0.0 1,418,621 + +0.2 1,418,621 + +1.0 1,418,744 + RR42_RS27035 0.51 +1.3 1,418,745 - RR42_RS27035 0.52 -1.5 1,418,751 - RR42_RS27035 0.55 -0.3 1,418,751 - RR42_RS27035 0.55 -1.0 1,419,317 - RR42_RS27045 0.52 -1.1 1,419,376 + RR42_RS27045 0.67 -0.5 1,419,417 + RR42_RS27045 0.77 +0.5
Or see this region's nucleotide sequence