Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05410 and RR42_RS05415 overlap by 4 nucleotides RR42_RS05415 and RR42_RS05420 are separated by 193 nucleotides RR42_RS05420 and RR42_RS05425 are separated by 2 nucleotides RR42_RS05425 and RR42_RS05430 overlap by 4 nucleotides
RR42_RS05410: RR42_RS05410 - acetyltransferase, at 1,195,339 to 1,196,316
_RS05410
RR42_RS05415: RR42_RS05415 - membrane protein, at 1,196,313 to 1,196,879
_RS05415
RR42_RS05420: RR42_RS05420 - membrane protein, at 1,197,073 to 1,197,852
_RS05420
RR42_RS05425: RR42_RS05425 - membrane protein, at 1,197,855 to 1,198,289
_RS05425
RR42_RS05430: RR42_RS05430 - DNA-directed RNA polymerase sigma-70 factor, at 1,198,286 to 1,198,852
_RS05430
Position (kb)
1197
1198 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1196.136 kb on + strand, within RR42_RS05410 at 1196.427 kb on + strand, within RR42_RS05415 at 1196.428 kb on - strand, within RR42_RS05415 at 1196.428 kb on - strand, within RR42_RS05415 at 1196.512 kb on + strand, within RR42_RS05415 at 1196.626 kb on + strand, within RR42_RS05415 at 1196.627 kb on - strand, within RR42_RS05415 at 1196.713 kb on + strand, within RR42_RS05415 at 1196.713 kb on + strand, within RR42_RS05415 at 1196.714 kb on - strand, within RR42_RS05415 at 1196.752 kb on - strand, within RR42_RS05415 at 1196.995 kb on + strand at 1196.996 kb on - strand at 1196.996 kb on - strand at 1196.996 kb on - strand at 1197.416 kb on + strand, within RR42_RS05420 at 1197.417 kb on - strand, within RR42_RS05420 at 1197.417 kb on - strand, within RR42_RS05420 at 1197.471 kb on - strand, within RR42_RS05420 at 1197.857 kb on - strand at 1197.857 kb on - strand at 1197.876 kb on + strand at 1197.876 kb on + strand at 1197.877 kb on - strand at 1197.899 kb on - strand, within RR42_RS05425 at 1197.982 kb on + strand, within RR42_RS05425 at 1197.982 kb on + strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1198.406 kb on - strand, within RR42_RS05430 at 1198.567 kb on + strand, within RR42_RS05430 at 1198.619 kb on - strand, within RR42_RS05430 at 1198.626 kb on - strand, within RR42_RS05430 at 1198.667 kb on + strand, within RR42_RS05430 at 1198.668 kb on - strand, within RR42_RS05430 at 1198.832 kb on + strand at 1198.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,196,136 + RR42_RS05410 0.81 +0.3 1,196,427 + RR42_RS05415 0.20 -0.6 1,196,428 - RR42_RS05415 0.20 +0.1 1,196,428 - RR42_RS05415 0.20 -0.5 1,196,512 + RR42_RS05415 0.35 +0.5 1,196,626 + RR42_RS05415 0.55 -0.0 1,196,627 - RR42_RS05415 0.55 +0.9 1,196,713 + RR42_RS05415 0.71 +1.0 1,196,713 + RR42_RS05415 0.71 -1.0 1,196,714 - RR42_RS05415 0.71 +0.6 1,196,752 - RR42_RS05415 0.77 -0.7 1,196,995 + -0.3 1,196,996 - +0.2 1,196,996 - +0.6 1,196,996 - +0.9 1,197,416 + RR42_RS05420 0.44 -1.4 1,197,417 - RR42_RS05420 0.44 -0.5 1,197,417 - RR42_RS05420 0.44 -0.7 1,197,471 - RR42_RS05420 0.51 -0.9 1,197,857 - -0.4 1,197,857 - -2.1 1,197,876 + -0.7 1,197,876 + +2.1 1,197,877 - +0.5 1,197,899 - RR42_RS05425 0.10 -0.9 1,197,982 + RR42_RS05425 0.29 +0.9 1,197,982 + RR42_RS05425 0.29 -0.4 1,197,983 - RR42_RS05425 0.29 +0.3 1,197,983 - RR42_RS05425 0.29 +0.3 1,197,983 - RR42_RS05425 0.29 +1.0 1,197,983 - RR42_RS05425 0.29 -0.6 1,197,983 - RR42_RS05425 0.29 +0.9 1,197,983 - RR42_RS05425 0.29 -0.3 1,197,983 - RR42_RS05425 0.29 +0.6 1,197,983 - RR42_RS05425 0.29 -0.1 1,198,406 - RR42_RS05430 0.21 +0.6 1,198,567 + RR42_RS05430 0.50 +0.1 1,198,619 - RR42_RS05430 0.59 -0.1 1,198,626 - RR42_RS05430 0.60 -0.5 1,198,667 + RR42_RS05430 0.67 +0.7 1,198,668 - RR42_RS05430 0.67 -0.2 1,198,832 + +0.1 1,198,832 + -0.7
Or see this region's nucleotide sequence