Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00925 and RR42_RS00930 overlap by 11 nucleotides RR42_RS00930 and RR42_RS00935 overlap by 4 nucleotides RR42_RS00935 and RR42_RS00940 are separated by 57 nucleotides
RR42_RS00925: RR42_RS00925 - adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA, at 220,801 to 222,288
_RS00925
RR42_RS00930: RR42_RS00930 - 8-amino-7-oxononanoate synthase, at 222,278 to 223,495
_RS00930
RR42_RS00935: RR42_RS00935 - dethiobiotin synthetase, at 223,492 to 224,214
_RS00935
RR42_RS00940: RR42_RS00940 - biotin synthase, at 224,272 to 225,318
_RS00940
Position (kb) 222
223
224 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 221.288 kb on - strand, within RR42_RS00925 at 221.348 kb on - strand, within RR42_RS00925 at 221.348 kb on - strand, within RR42_RS00925 at 221.348 kb on - strand, within RR42_RS00925 at 221.851 kb on + strand, within RR42_RS00925 at 221.851 kb on + strand, within RR42_RS00925 at 222.000 kb on - strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.043 kb on + strand, within RR42_RS00925 at 222.044 kb on - strand, within RR42_RS00925 at 222.133 kb on + strand, within RR42_RS00925 at 222.133 kb on + strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.134 kb on - strand, within RR42_RS00925 at 222.149 kb on - strand at 222.149 kb on - strand at 222.149 kb on - strand at 222.272 kb on + strand at 222.273 kb on - strand at 222.273 kb on - strand at 222.353 kb on + strand at 222.354 kb on - strand at 222.614 kb on + strand, within RR42_RS00930 at 222.629 kb on + strand, within RR42_RS00930 at 222.629 kb on + strand, within RR42_RS00930 at 222.629 kb on + strand, within RR42_RS00930 at 222.631 kb on + strand, within RR42_RS00930 at 222.631 kb on + strand, within RR42_RS00930 at 222.632 kb on - strand, within RR42_RS00930 at 222.632 kb on - strand, within RR42_RS00930 at 222.632 kb on - strand, within RR42_RS00930 at 222.661 kb on - strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.998 kb on + strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 222.999 kb on - strand, within RR42_RS00930 at 223.094 kb on + strand, within RR42_RS00930 at 223.094 kb on + strand, within RR42_RS00930 at 223.094 kb on + strand, within RR42_RS00930 at 223.095 kb on - strand, within RR42_RS00930 at 223.100 kb on + strand, within RR42_RS00930 at 223.100 kb on + strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 223.101 kb on - strand, within RR42_RS00930 at 224.252 kb on + strand at 224.410 kb on + strand, within RR42_RS00940 at 224.410 kb on + strand, within RR42_RS00940
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 221,288 - RR42_RS00925 0.33 -0.7 221,348 - RR42_RS00925 0.37 -1.0 221,348 - RR42_RS00925 0.37 -0.8 221,348 - RR42_RS00925 0.37 -2.2 221,851 + RR42_RS00925 0.71 -1.5 221,851 + RR42_RS00925 0.71 -0.6 222,000 - RR42_RS00925 0.81 -1.4 222,043 + RR42_RS00925 0.83 -0.7 222,043 + RR42_RS00925 0.83 -1.1 222,043 + RR42_RS00925 0.83 -0.2 222,043 + RR42_RS00925 0.83 -1.6 222,044 - RR42_RS00925 0.84 -2.8 222,133 + RR42_RS00925 0.90 -2.2 222,133 + RR42_RS00925 0.90 -2.3 222,134 - RR42_RS00925 0.90 -1.3 222,134 - RR42_RS00925 0.90 -1.4 222,134 - RR42_RS00925 0.90 +0.3 222,134 - RR42_RS00925 0.90 -0.8 222,134 - RR42_RS00925 0.90 +0.3 222,134 - RR42_RS00925 0.90 -3.9 222,149 - -0.4 222,149 - -0.5 222,149 - -0.4 222,272 + +0.4 222,273 - +1.0 222,273 - -1.3 222,353 + -2.6 222,354 - -1.0 222,614 + RR42_RS00930 0.28 +0.8 222,629 + RR42_RS00930 0.29 -0.8 222,629 + RR42_RS00930 0.29 -1.2 222,629 + RR42_RS00930 0.29 -0.9 222,631 + RR42_RS00930 0.29 -0.5 222,631 + RR42_RS00930 0.29 -0.1 222,632 - RR42_RS00930 0.29 -0.5 222,632 - RR42_RS00930 0.29 -1.5 222,632 - RR42_RS00930 0.29 -1.1 222,661 - RR42_RS00930 0.31 -1.9 222,998 + RR42_RS00930 0.59 -0.9 222,998 + RR42_RS00930 0.59 -1.0 222,998 + RR42_RS00930 0.59 +0.5 222,998 + RR42_RS00930 0.59 -0.3 222,999 - RR42_RS00930 0.59 -1.7 222,999 - RR42_RS00930 0.59 -2.1 222,999 - RR42_RS00930 0.59 -1.2 222,999 - RR42_RS00930 0.59 -3.6 222,999 - RR42_RS00930 0.59 -1.5 222,999 - RR42_RS00930 0.59 -0.4 222,999 - RR42_RS00930 0.59 -1.0 222,999 - RR42_RS00930 0.59 -2.3 222,999 - RR42_RS00930 0.59 -0.4 222,999 - RR42_RS00930 0.59 -1.1 223,094 + RR42_RS00930 0.67 -0.0 223,094 + RR42_RS00930 0.67 +0.3 223,094 + RR42_RS00930 0.67 +0.8 223,095 - RR42_RS00930 0.67 -2.2 223,100 + RR42_RS00930 0.67 +0.1 223,100 + RR42_RS00930 0.67 -0.0 223,101 - RR42_RS00930 0.68 -0.3 223,101 - RR42_RS00930 0.68 -0.2 223,101 - RR42_RS00930 0.68 -1.3 223,101 - RR42_RS00930 0.68 -1.8 223,101 - RR42_RS00930 0.68 -3.2 223,101 - RR42_RS00930 0.68 -0.6 224,252 + +1.0 224,410 + RR42_RS00940 0.13 -1.7 224,410 + RR42_RS00940 0.13 -2.8
Or see this region's nucleotide sequence