Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08070 and MMJJ_RS08075 are separated by 122 nucleotides MMJJ_RS08075 and MMJJ_RS08080 are separated by 126 nucleotides
MMJJ_RS08070: MMJJ_RS08070 - alpha-glucan family phosphorylase, at 1,477,962 to 1,479,524
_RS08070
MMJJ_RS08075: MMJJ_RS08075 - ATP-dependent DNA helicase, at 1,479,647 to 1,481,656
_RS08075
MMJJ_RS08080: MMJJ_RS08080 - TIGR00300 family protein, at 1,481,783 to 1,483,030
_RS08080
Position (kb)
1479
1480
1481
1482 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1478.762 kb on - strand, within MMJJ_RS08070 at 1478.793 kb on - strand, within MMJJ_RS08070 at 1478.793 kb on - strand, within MMJJ_RS08070 at 1478.793 kb on - strand, within MMJJ_RS08070 at 1478.794 kb on + strand, within MMJJ_RS08070 at 1478.968 kb on + strand, within MMJJ_RS08070 at 1478.968 kb on + strand, within MMJJ_RS08070 at 1479.009 kb on + strand, within MMJJ_RS08070 at 1479.086 kb on - strand, within MMJJ_RS08070 at 1479.148 kb on + strand, within MMJJ_RS08070 at 1479.148 kb on + strand, within MMJJ_RS08070 at 1479.155 kb on + strand, within MMJJ_RS08070 at 1479.172 kb on - strand, within MMJJ_RS08070 at 1479.173 kb on + strand, within MMJJ_RS08070 at 1479.191 kb on + strand, within MMJJ_RS08070 at 1479.191 kb on + strand, within MMJJ_RS08070 at 1479.191 kb on + strand, within MMJJ_RS08070 at 1479.191 kb on + strand, within MMJJ_RS08070 at 1479.276 kb on + strand, within MMJJ_RS08070 at 1479.329 kb on - strand, within MMJJ_RS08070 at 1479.381 kb on - strand at 1479.384 kb on + strand at 1479.402 kb on + strand at 1479.402 kb on + strand at 1479.403 kb on - strand at 1479.404 kb on + strand at 1479.412 kb on + strand at 1479.469 kb on + strand at 1479.501 kb on + strand at 1479.523 kb on + strand at 1479.584 kb on - strand at 1479.585 kb on + strand at 1479.585 kb on + strand at 1479.585 kb on + strand at 1479.588 kb on - strand at 1479.588 kb on - strand at 1479.588 kb on - strand at 1479.588 kb on - strand at 1479.588 kb on - strand at 1479.588 kb on - strand at 1479.588 kb on - strand at 1479.589 kb on + strand at 1479.590 kb on - strand at 1479.590 kb on - strand at 1479.591 kb on + strand at 1479.591 kb on + strand at 1479.591 kb on + strand at 1479.591 kb on + strand at 1479.593 kb on + strand at 1479.607 kb on - strand at 1479.625 kb on + strand at 1479.635 kb on - strand at 1479.825 kb on + strand at 1480.003 kb on + strand, within MMJJ_RS08075 at 1480.088 kb on + strand, within MMJJ_RS08075 at 1480.156 kb on - strand, within MMJJ_RS08075 at 1480.157 kb on + strand, within MMJJ_RS08075 at 1480.157 kb on + strand, within MMJJ_RS08075 at 1480.178 kb on + strand, within MMJJ_RS08075 at 1480.178 kb on + strand, within MMJJ_RS08075 at 1480.191 kb on - strand, within MMJJ_RS08075 at 1480.192 kb on + strand, within MMJJ_RS08075 at 1480.192 kb on + strand, within MMJJ_RS08075 at 1480.231 kb on + strand, within MMJJ_RS08075 at 1480.290 kb on - strand, within MMJJ_RS08075 at 1480.729 kb on - strand, within MMJJ_RS08075 at 1480.730 kb on + strand at 1480.747 kb on + strand, within MMJJ_RS08075 at 1480.751 kb on - strand, within MMJJ_RS08075 at 1480.752 kb on + strand, within MMJJ_RS08075 at 1480.752 kb on + strand, within MMJJ_RS08075 at 1480.753 kb on - strand, within MMJJ_RS08075 at 1480.754 kb on + strand, within MMJJ_RS08075 at 1480.808 kb on + strand, within MMJJ_RS08075 at 1480.830 kb on - strand, within MMJJ_RS08075 at 1480.905 kb on - strand, within MMJJ_RS08075 at 1480.906 kb on + strand, within MMJJ_RS08075 at 1480.944 kb on - strand, within MMJJ_RS08075 at 1480.944 kb on - strand, within MMJJ_RS08075 at 1480.944 kb on - strand, within MMJJ_RS08075 at 1480.945 kb on + strand, within MMJJ_RS08075 at 1480.986 kb on - strand, within MMJJ_RS08075 at 1480.987 kb on + strand, within MMJJ_RS08075 at 1481.005 kb on - strand, within MMJJ_RS08075 at 1481.039 kb on + strand, within MMJJ_RS08075 at 1481.065 kb on - strand, within MMJJ_RS08075 at 1481.114 kb on - strand, within MMJJ_RS08075 at 1481.122 kb on + strand, within MMJJ_RS08075 at 1481.122 kb on + strand, within MMJJ_RS08075 at 1481.143 kb on - strand, within MMJJ_RS08075 at 1481.143 kb on - strand, within MMJJ_RS08075 at 1481.143 kb on - strand, within MMJJ_RS08075 at 1481.143 kb on - strand, within MMJJ_RS08075 at 1481.144 kb on + strand, within MMJJ_RS08075 at 1481.144 kb on + strand, within MMJJ_RS08075 at 1481.144 kb on + strand, within MMJJ_RS08075 at 1481.145 kb on - strand, within MMJJ_RS08075 at 1481.146 kb on + strand, within MMJJ_RS08075 at 1481.146 kb on + strand, within MMJJ_RS08075 at 1481.146 kb on + strand, within MMJJ_RS08075 at 1481.147 kb on - strand, within MMJJ_RS08075 at 1481.147 kb on - strand, within MMJJ_RS08075 at 1481.147 kb on - strand, within MMJJ_RS08075 at 1481.148 kb on + strand, within MMJJ_RS08075 at 1481.148 kb on + strand, within MMJJ_RS08075 at 1481.148 kb on + strand, within MMJJ_RS08075 at 1481.148 kb on + strand, within MMJJ_RS08075 at 1481.153 kb on - strand, within MMJJ_RS08075 at 1481.227 kb on + strand, within MMJJ_RS08075 at 1481.284 kb on - strand, within MMJJ_RS08075 at 1481.286 kb on - strand, within MMJJ_RS08075 at 1481.289 kb on - strand, within MMJJ_RS08075 at 1481.290 kb on + strand, within MMJJ_RS08075 at 1481.290 kb on + strand, within MMJJ_RS08075 at 1481.294 kb on + strand, within MMJJ_RS08075 at 1481.418 kb on - strand, within MMJJ_RS08075 at 1481.434 kb on - strand, within MMJJ_RS08075 at 1481.436 kb on - strand, within MMJJ_RS08075 at 1481.437 kb on + strand, within MMJJ_RS08075 at 1481.507 kb on + strand at 1481.579 kb on + strand at 1481.585 kb on + strand at 1481.591 kb on - strand at 1481.591 kb on - strand at 1481.592 kb on + strand at 1481.638 kb on - strand at 1481.638 kb on - strand at 1481.692 kb on - strand at 1481.693 kb on + strand at 1481.693 kb on + strand at 1481.693 kb on + strand at 1481.693 kb on + strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.700 kb on - strand at 1481.701 kb on + strand at 1481.701 kb on + strand at 1481.701 kb on + strand at 1481.701 kb on + strand at 1481.701 kb on + strand at 1481.701 kb on + strand at 1481.702 kb on - strand at 1481.702 kb on - strand at 1481.702 kb on - strand at 1481.702 kb on - strand at 1481.703 kb on + strand at 1481.703 kb on + strand at 1481.703 kb on + strand at 1481.705 kb on - strand at 1481.705 kb on - strand at 1481.705 kb on - strand at 1481.705 kb on - strand at 1481.705 kb on - strand at 1481.706 kb on + strand at 1481.706 kb on + strand at 1481.719 kb on - strand at 1481.719 kb on - strand at 1481.722 kb on + strand at 1481.722 kb on + strand at 1481.723 kb on - strand at 1481.723 kb on - strand at 1481.723 kb on - strand at 1481.724 kb on + strand at 1481.724 kb on + strand at 1481.724 kb on + strand at 1481.724 kb on + strand at 1481.732 kb on + strand at 1481.732 kb on + strand at 1481.739 kb on + strand at 1481.749 kb on + strand at 1481.818 kb on + strand at 1481.818 kb on + strand at 1481.818 kb on + strand at 1481.830 kb on - strand at 1481.831 kb on + strand at 1481.832 kb on - strand at 1481.833 kb on + strand at 1481.833 kb on + strand at 1481.878 kb on - strand at 1481.879 kb on + strand at 1482.080 kb on + strand, within MMJJ_RS08080 at 1482.080 kb on + strand, within MMJJ_RS08080 at 1482.100 kb on + strand, within MMJJ_RS08080 at 1482.106 kb on + strand, within MMJJ_RS08080 at 1482.150 kb on - strand, within MMJJ_RS08080 at 1482.151 kb on + strand, within MMJJ_RS08080 at 1482.204 kb on - strand, within MMJJ_RS08080 at 1482.370 kb on + strand, within MMJJ_RS08080 at 1482.381 kb on + strand, within MMJJ_RS08080 at 1482.433 kb on + strand, within MMJJ_RS08080 at 1482.433 kb on + strand, within MMJJ_RS08080 at 1482.537 kb on - strand, within MMJJ_RS08080 at 1482.565 kb on + strand, within MMJJ_RS08080 at 1482.615 kb on - strand, within MMJJ_RS08080 at 1482.616 kb on + strand, within MMJJ_RS08080 at 1482.625 kb on + strand, within MMJJ_RS08080
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 1,478,762 - MMJJ_RS08070 0.51 +0.6 1,478,793 - MMJJ_RS08070 0.53 +0.3 1,478,793 - MMJJ_RS08070 0.53 -0.5 1,478,793 - MMJJ_RS08070 0.53 +0.3 1,478,794 + MMJJ_RS08070 0.53 -0.5 1,478,968 + MMJJ_RS08070 0.64 +0.1 1,478,968 + MMJJ_RS08070 0.64 +0.5 1,479,009 + MMJJ_RS08070 0.67 +0.0 1,479,086 - MMJJ_RS08070 0.72 -0.7 1,479,148 + MMJJ_RS08070 0.76 -0.3 1,479,148 + MMJJ_RS08070 0.76 -0.4 1,479,155 + MMJJ_RS08070 0.76 -0.8 1,479,172 - MMJJ_RS08070 0.77 +1.2 1,479,173 + MMJJ_RS08070 0.77 -0.8 1,479,191 + MMJJ_RS08070 0.79 -0.4 1,479,191 + MMJJ_RS08070 0.79 -0.7 1,479,191 + MMJJ_RS08070 0.79 -1.0 1,479,191 + MMJJ_RS08070 0.79 +0.2 1,479,276 + MMJJ_RS08070 0.84 +0.3 1,479,329 - MMJJ_RS08070 0.87 -0.6 1,479,381 - +0.6 1,479,384 + +0.3 1,479,402 + +0.1 1,479,402 + -1.3 1,479,403 - +0.4 1,479,404 + +0.2 1,479,412 + +0.8 1,479,469 + -2.0 1,479,501 + +0.1 1,479,523 + -0.5 1,479,584 - -0.8 1,479,585 + +0.2 1,479,585 + -0.3 1,479,585 + +0.6 1,479,588 - +0.5 1,479,588 - +0.3 1,479,588 - -0.1 1,479,588 - +0.2 1,479,588 - +0.4 1,479,588 - +0.2 1,479,588 - -0.1 1,479,589 + +0.9 1,479,590 - -0.2 1,479,590 - +0.5 1,479,591 + +0.7 1,479,591 + +1.1 1,479,591 + -0.2 1,479,591 + +0.6 1,479,593 + -0.5 1,479,607 - +0.0 1,479,625 + +1.3 1,479,635 - +0.1 1,479,825 + -1.9 1,480,003 + MMJJ_RS08075 0.18 +0.6 1,480,088 + MMJJ_RS08075 0.22 +1.0 1,480,156 - MMJJ_RS08075 0.25 -0.0 1,480,157 + MMJJ_RS08075 0.25 -0.0 1,480,157 + MMJJ_RS08075 0.25 +1.2 1,480,178 + MMJJ_RS08075 0.26 +0.2 1,480,178 + MMJJ_RS08075 0.26 +1.0 1,480,191 - MMJJ_RS08075 0.27 -0.2 1,480,192 + MMJJ_RS08075 0.27 +0.3 1,480,192 + MMJJ_RS08075 0.27 -1.1 1,480,231 + MMJJ_RS08075 0.29 -0.4 1,480,290 - MMJJ_RS08075 0.32 +1.0 1,480,729 - MMJJ_RS08075 0.54 -0.6 1,480,730 + -0.1 1,480,747 + MMJJ_RS08075 0.55 -0.2 1,480,751 - MMJJ_RS08075 0.55 -0.9 1,480,752 + MMJJ_RS08075 0.55 -0.5 1,480,752 + MMJJ_RS08075 0.55 -0.4 1,480,753 - MMJJ_RS08075 0.55 +1.2 1,480,754 + MMJJ_RS08075 0.55 -0.3 1,480,808 + MMJJ_RS08075 0.58 +0.2 1,480,830 - MMJJ_RS08075 0.59 +0.5 1,480,905 - MMJJ_RS08075 0.63 -0.1 1,480,906 + MMJJ_RS08075 0.63 +0.1 1,480,944 - MMJJ_RS08075 0.65 +0.3 1,480,944 - MMJJ_RS08075 0.65 -0.0 1,480,944 - MMJJ_RS08075 0.65 -0.0 1,480,945 + MMJJ_RS08075 0.65 -0.0 1,480,986 - MMJJ_RS08075 0.67 -0.2 1,480,987 + MMJJ_RS08075 0.67 +0.6 1,481,005 - MMJJ_RS08075 0.68 +0.5 1,481,039 + MMJJ_RS08075 0.69 +1.0 1,481,065 - MMJJ_RS08075 0.71 -0.1 1,481,114 - MMJJ_RS08075 0.73 +0.2 1,481,122 + MMJJ_RS08075 0.73 -0.4 1,481,122 + MMJJ_RS08075 0.73 -0.3 1,481,143 - MMJJ_RS08075 0.74 -0.1 1,481,143 - MMJJ_RS08075 0.74 +0.2 1,481,143 - MMJJ_RS08075 0.74 -0.5 1,481,143 - MMJJ_RS08075 0.74 +0.0 1,481,144 + MMJJ_RS08075 0.74 -0.1 1,481,144 + MMJJ_RS08075 0.74 +1.1 1,481,144 + MMJJ_RS08075 0.74 +0.2 1,481,145 - MMJJ_RS08075 0.75 +0.0 1,481,146 + MMJJ_RS08075 0.75 +1.6 1,481,146 + MMJJ_RS08075 0.75 -0.1 1,481,146 + MMJJ_RS08075 0.75 +0.6 1,481,147 - MMJJ_RS08075 0.75 +0.9 1,481,147 - MMJJ_RS08075 0.75 -0.1 1,481,147 - MMJJ_RS08075 0.75 +0.1 1,481,148 + MMJJ_RS08075 0.75 +0.4 1,481,148 + MMJJ_RS08075 0.75 -0.0 1,481,148 + MMJJ_RS08075 0.75 -0.1 1,481,148 + MMJJ_RS08075 0.75 -0.4 1,481,153 - MMJJ_RS08075 0.75 -0.2 1,481,227 + MMJJ_RS08075 0.79 -0.1 1,481,284 - MMJJ_RS08075 0.81 +0.3 1,481,286 - MMJJ_RS08075 0.82 +0.1 1,481,289 - MMJJ_RS08075 0.82 +0.0 1,481,290 + MMJJ_RS08075 0.82 +0.0 1,481,290 + MMJJ_RS08075 0.82 +0.5 1,481,294 + MMJJ_RS08075 0.82 +0.4 1,481,418 - MMJJ_RS08075 0.88 +0.3 1,481,434 - MMJJ_RS08075 0.89 -0.0 1,481,436 - MMJJ_RS08075 0.89 +0.4 1,481,437 + MMJJ_RS08075 0.89 -0.5 1,481,507 + +0.0 1,481,579 + +0.1 1,481,585 + +0.3 1,481,591 - +0.2 1,481,591 - -1.4 1,481,592 + -0.6 1,481,638 - +1.1 1,481,638 - +0.0 1,481,692 - -0.7 1,481,693 + -0.7 1,481,693 + -0.8 1,481,693 + -0.1 1,481,693 + -0.1 1,481,700 - -0.1 1,481,700 - +0.2 1,481,700 - -0.4 1,481,700 - -0.2 1,481,700 - +0.1 1,481,700 - -1.0 1,481,700 - +0.4 1,481,700 - +0.4 1,481,700 - -0.2 1,481,700 - +0.0 1,481,700 - +0.5 1,481,700 - -0.1 1,481,701 + +0.1 1,481,701 + +0.4 1,481,701 + +0.5 1,481,701 + -0.7 1,481,701 + +0.3 1,481,701 + -0.8 1,481,702 - +0.2 1,481,702 - +0.5 1,481,702 - -1.4 1,481,702 - +0.1 1,481,703 + -0.4 1,481,703 + +1.3 1,481,703 + -0.3 1,481,705 - -1.7 1,481,705 - -0.5 1,481,705 - -0.1 1,481,705 - -0.4 1,481,705 - +0.2 1,481,706 + +1.2 1,481,706 + +0.2 1,481,719 - +0.3 1,481,719 - +0.3 1,481,722 + -0.8 1,481,722 + -0.8 1,481,723 - -1.8 1,481,723 - -0.1 1,481,723 - -0.7 1,481,724 + -1.3 1,481,724 + -0.7 1,481,724 + -0.8 1,481,724 + -0.6 1,481,732 + -0.5 1,481,732 + -3.9 1,481,739 + -3.5 1,481,749 + -0.5 1,481,818 + -3.1 1,481,818 + -2.8 1,481,818 + -4.2 1,481,830 - -0.2 1,481,831 + -2.2 1,481,832 - -1.7 1,481,833 + +0.1 1,481,833 + -1.9 1,481,878 - -2.7 1,481,879 + -2.2 1,482,080 + MMJJ_RS08080 0.24 -2.4 1,482,080 + MMJJ_RS08080 0.24 -2.5 1,482,100 + MMJJ_RS08080 0.25 -2.2 1,482,106 + MMJJ_RS08080 0.26 -3.1 1,482,150 - MMJJ_RS08080 0.29 +0.3 1,482,151 + MMJJ_RS08080 0.29 -2.1 1,482,204 - MMJJ_RS08080 0.34 -1.7 1,482,370 + MMJJ_RS08080 0.47 -2.8 1,482,381 + MMJJ_RS08080 0.48 -2.2 1,482,433 + MMJJ_RS08080 0.52 -3.8 1,482,433 + MMJJ_RS08080 0.52 -4.2 1,482,537 - MMJJ_RS08080 0.60 -0.9 1,482,565 + MMJJ_RS08080 0.63 -2.5 1,482,615 - MMJJ_RS08080 0.67 -3.3 1,482,616 + MMJJ_RS08080 0.67 -2.6 1,482,625 + MMJJ_RS08080 0.67 -3.7
Or see this region's nucleotide sequence