Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01910 and MMJJ_RS01915 are separated by 34 nucleotides MMJJ_RS01915 and MMJJ_RS01920 are separated by 4 nucleotides MMJJ_RS01920 and MMJJ_RS01925 are separated by 157 nucleotides MMJJ_RS01925 and MMJJ_RS01930 are separated by 17 nucleotides
MMJJ_RS01910: MMJJ_RS01910 - class III signal peptide-containing protein, at 365,935 to 366,618
_RS01910
MMJJ_RS01915: MMJJ_RS01915 - precorrin-6A reductase, at 366,653 to 367,399
_RS01915
MMJJ_RS01920: MMJJ_RS01920 - phosphoglycerate mutase, at 367,404 to 368,690
_RS01920
MMJJ_RS01925: MMJJ_RS01925 - fibrillarin-like rRNA/tRNA 2'-O-methyltransferase, at 368,848 to 369,540
_RS01925
MMJJ_RS01930: MMJJ_RS01930 - Pre-mRNA processing ribonucleoprotein, at 369,558 to 370,988
_RS01930
Position (kb) 367
368
369 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 366.440 kb on - strand, within MMJJ_RS01910 at 366.445 kb on - strand, within MMJJ_RS01910 at 366.446 kb on + strand, within MMJJ_RS01910 at 366.491 kb on + strand, within MMJJ_RS01910 at 366.494 kb on - strand, within MMJJ_RS01910 at 366.495 kb on + strand, within MMJJ_RS01910 at 366.515 kb on - strand, within MMJJ_RS01910 at 366.516 kb on + strand, within MMJJ_RS01910 at 366.521 kb on - strand, within MMJJ_RS01910 at 366.522 kb on + strand, within MMJJ_RS01910 at 366.559 kb on + strand at 366.568 kb on + strand at 366.583 kb on + strand at 366.584 kb on - strand at 366.584 kb on - strand at 366.585 kb on + strand at 366.598 kb on - strand at 366.598 kb on - strand at 366.600 kb on - strand at 366.600 kb on - strand at 366.600 kb on - strand at 366.601 kb on + strand at 366.601 kb on + strand at 366.623 kb on + strand at 366.625 kb on - strand at 366.626 kb on + strand at 366.660 kb on - strand at 366.660 kb on - strand at 366.661 kb on + strand at 366.680 kb on + strand at 366.695 kb on + strand at 366.796 kb on + strand, within MMJJ_RS01915 at 366.815 kb on - strand, within MMJJ_RS01915 at 366.816 kb on + strand, within MMJJ_RS01915 at 366.816 kb on + strand, within MMJJ_RS01915 at 366.820 kb on - strand, within MMJJ_RS01915 at 366.821 kb on + strand, within MMJJ_RS01915 at 366.938 kb on - strand, within MMJJ_RS01915 at 366.939 kb on + strand, within MMJJ_RS01915 at 366.984 kb on + strand, within MMJJ_RS01915 at 367.015 kb on + strand, within MMJJ_RS01915 at 367.047 kb on + strand, within MMJJ_RS01915 at 367.087 kb on - strand, within MMJJ_RS01915 at 367.116 kb on - strand, within MMJJ_RS01915 at 367.150 kb on + strand, within MMJJ_RS01915 at 367.212 kb on - strand, within MMJJ_RS01915 at 367.215 kb on - strand, within MMJJ_RS01915 at 367.225 kb on - strand, within MMJJ_RS01915 at 367.226 kb on + strand, within MMJJ_RS01915 at 367.236 kb on - strand, within MMJJ_RS01915 at 367.237 kb on + strand, within MMJJ_RS01915 at 367.237 kb on + strand, within MMJJ_RS01915 at 367.322 kb on + strand, within MMJJ_RS01915 at 367.323 kb on - strand, within MMJJ_RS01915 at 367.375 kb on + strand at 367.382 kb on - strand at 367.382 kb on - strand at 367.397 kb on - strand at 367.402 kb on + strand at 367.411 kb on + strand at 367.419 kb on - strand at 367.419 kb on - strand at 367.419 kb on - strand at 367.419 kb on - strand at 367.420 kb on + strand at 367.420 kb on + strand at 367.420 kb on + strand at 367.420 kb on + strand at 367.420 kb on + strand at 367.420 kb on + strand at 367.421 kb on - strand at 367.441 kb on - strand at 367.474 kb on - strand at 367.475 kb on + strand at 367.476 kb on - strand at 367.476 kb on - strand at 367.477 kb on + strand at 367.477 kb on + strand at 367.480 kb on + strand at 367.550 kb on + strand, within MMJJ_RS01920 at 367.577 kb on - strand, within MMJJ_RS01920 at 367.577 kb on - strand, within MMJJ_RS01920 at 367.608 kb on - strand, within MMJJ_RS01920 at 367.608 kb on - strand, within MMJJ_RS01920 at 367.678 kb on + strand, within MMJJ_RS01920 at 367.678 kb on + strand, within MMJJ_RS01920 at 367.681 kb on + strand, within MMJJ_RS01920 at 367.686 kb on - strand, within MMJJ_RS01920 at 367.686 kb on - strand, within MMJJ_RS01920 at 367.686 kb on - strand, within MMJJ_RS01920 at 367.686 kb on - strand, within MMJJ_RS01920 at 367.687 kb on + strand, within MMJJ_RS01920 at 367.695 kb on - strand, within MMJJ_RS01920 at 367.704 kb on + strand, within MMJJ_RS01920 at 367.734 kb on - strand, within MMJJ_RS01920 at 367.738 kb on + strand, within MMJJ_RS01920 at 367.798 kb on - strand, within MMJJ_RS01920 at 367.798 kb on - strand, within MMJJ_RS01920 at 367.798 kb on - strand, within MMJJ_RS01920 at 367.799 kb on + strand, within MMJJ_RS01920 at 367.799 kb on + strand, within MMJJ_RS01920 at 367.799 kb on + strand, within MMJJ_RS01920 at 367.799 kb on + strand, within MMJJ_RS01920 at 367.804 kb on + strand, within MMJJ_RS01920 at 367.804 kb on + strand, within MMJJ_RS01920 at 367.808 kb on + strand, within MMJJ_RS01920 at 367.810 kb on - strand, within MMJJ_RS01920 at 367.810 kb on - strand, within MMJJ_RS01920 at 367.811 kb on + strand, within MMJJ_RS01920 at 367.815 kb on + strand, within MMJJ_RS01920 at 367.863 kb on + strand, within MMJJ_RS01920 at 367.866 kb on - strand, within MMJJ_RS01920 at 367.866 kb on - strand, within MMJJ_RS01920 at 367.867 kb on + strand, within MMJJ_RS01920 at 367.867 kb on + strand, within MMJJ_RS01920 at 367.870 kb on - strand, within MMJJ_RS01920 at 367.881 kb on - strand, within MMJJ_RS01920 at 367.910 kb on - strand, within MMJJ_RS01920 at 367.936 kb on + strand, within MMJJ_RS01920 at 367.943 kb on + strand, within MMJJ_RS01920 at 367.965 kb on + strand, within MMJJ_RS01920 at 368.016 kb on + strand, within MMJJ_RS01920 at 368.016 kb on + strand, within MMJJ_RS01920 at 368.037 kb on + strand, within MMJJ_RS01920 at 368.117 kb on - strand, within MMJJ_RS01920 at 368.117 kb on - strand, within MMJJ_RS01920 at 368.118 kb on + strand, within MMJJ_RS01920 at 368.139 kb on - strand, within MMJJ_RS01920 at 368.140 kb on + strand, within MMJJ_RS01920 at 368.140 kb on + strand, within MMJJ_RS01920 at 368.167 kb on + strand at 368.167 kb on + strand, within MMJJ_RS01920 at 368.228 kb on - strand, within MMJJ_RS01920 at 368.307 kb on + strand, within MMJJ_RS01920 at 368.333 kb on - strand, within MMJJ_RS01920 at 368.333 kb on - strand, within MMJJ_RS01920 at 368.357 kb on - strand, within MMJJ_RS01920 at 368.362 kb on + strand, within MMJJ_RS01920 at 368.362 kb on + strand, within MMJJ_RS01920 at 368.377 kb on - strand, within MMJJ_RS01920 at 368.378 kb on + strand, within MMJJ_RS01920 at 368.378 kb on + strand, within MMJJ_RS01920 at 368.378 kb on + strand, within MMJJ_RS01920 at 368.378 kb on + strand, within MMJJ_RS01920 at 368.388 kb on - strand, within MMJJ_RS01920 at 368.416 kb on - strand, within MMJJ_RS01920 at 368.417 kb on + strand, within MMJJ_RS01920 at 368.432 kb on - strand, within MMJJ_RS01920 at 368.470 kb on + strand at 368.470 kb on + strand, within MMJJ_RS01920 at 368.511 kb on - strand, within MMJJ_RS01920 at 368.556 kb on - strand, within MMJJ_RS01920 at 368.617 kb on + strand at 368.704 kb on + strand at 368.707 kb on - strand at 368.708 kb on + strand at 368.708 kb on + strand at 368.711 kb on + strand at 368.719 kb on - strand at 368.720 kb on + strand at 368.720 kb on + strand at 368.729 kb on + strand at 368.729 kb on + strand at 368.729 kb on + strand at 368.736 kb on - strand at 368.737 kb on + strand at 368.740 kb on + strand at 368.748 kb on - strand at 368.750 kb on - strand at 368.750 kb on - strand at 368.751 kb on + strand at 368.751 kb on + strand at 368.751 kb on + strand at 368.752 kb on - strand at 368.752 kb on - strand at 368.752 kb on - strand at 368.753 kb on + strand at 368.758 kb on - strand at 368.765 kb on + strand at 368.784 kb on - strand at 368.784 kb on - strand at 368.785 kb on + strand at 368.786 kb on - strand at 368.787 kb on + strand at 368.787 kb on + strand at 368.788 kb on - strand at 368.788 kb on - strand at 368.788 kb on - strand at 368.788 kb on - strand at 368.789 kb on + strand at 368.789 kb on + strand at 368.807 kb on - strand at 368.823 kb on + strand at 368.828 kb on - strand at 368.836 kb on - strand at 368.836 kb on - strand at 368.840 kb on + strand at 368.842 kb on + strand at 368.849 kb on - strand at 368.849 kb on - strand at 368.985 kb on - strand, within MMJJ_RS01925 at 369.010 kb on + strand, within MMJJ_RS01925 at 369.237 kb on + strand, within MMJJ_RS01925 at 369.258 kb on - strand, within MMJJ_RS01925 at 369.280 kb on + strand, within MMJJ_RS01925 at 369.388 kb on - strand, within MMJJ_RS01925 at 369.393 kb on - strand, within MMJJ_RS01925 at 369.395 kb on - strand, within MMJJ_RS01925 at 369.395 kb on - strand, within MMJJ_RS01925 at 369.400 kb on - strand, within MMJJ_RS01925 at 369.400 kb on - strand, within MMJJ_RS01925 at 369.400 kb on - strand, within MMJJ_RS01925 at 369.400 kb on - strand, within MMJJ_RS01925 at 369.401 kb on + strand, within MMJJ_RS01925 at 369.402 kb on - strand, within MMJJ_RS01925 at 369.414 kb on + strand, within MMJJ_RS01925 at 369.414 kb on + strand, within MMJJ_RS01925 at 369.415 kb on - strand, within MMJJ_RS01925 at 369.415 kb on - strand, within MMJJ_RS01925 at 369.418 kb on - strand, within MMJJ_RS01925 at 369.451 kb on - strand, within MMJJ_RS01925 at 369.475 kb on + strand at 369.475 kb on + strand at 369.502 kb on - strand at 369.502 kb on - strand at 369.502 kb on - strand at 369.502 kb on - strand at 369.502 kb on - strand at 369.503 kb on + strand at 369.503 kb on + strand at 369.503 kb on + strand at 369.503 kb on + strand at 369.503 kb on + strand at 369.559 kb on - strand at 369.560 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 366,440 - MMJJ_RS01910 0.74 -0.0 366,445 - MMJJ_RS01910 0.75 +0.3 366,446 + MMJJ_RS01910 0.75 +0.3 366,491 + MMJJ_RS01910 0.81 -0.1 366,494 - MMJJ_RS01910 0.82 -0.2 366,495 + MMJJ_RS01910 0.82 -0.6 366,515 - MMJJ_RS01910 0.85 -0.3 366,516 + MMJJ_RS01910 0.85 -0.0 366,521 - MMJJ_RS01910 0.86 +0.3 366,522 + MMJJ_RS01910 0.86 -0.4 366,559 + +0.7 366,568 + +1.0 366,583 + +0.3 366,584 - +0.2 366,584 - -0.2 366,585 + -0.5 366,598 - +0.7 366,598 - -0.4 366,600 - +0.3 366,600 - -0.2 366,600 - -0.6 366,601 + -0.1 366,601 + -0.2 366,623 + -0.1 366,625 - +0.2 366,626 + -0.2 366,660 - -0.9 366,660 - -1.8 366,661 + -1.0 366,680 + -0.8 366,695 + -2.8 366,796 + MMJJ_RS01915 0.19 -0.4 366,815 - MMJJ_RS01915 0.22 -0.4 366,816 + MMJJ_RS01915 0.22 +0.3 366,816 + MMJJ_RS01915 0.22 -1.0 366,820 - MMJJ_RS01915 0.22 -0.8 366,821 + MMJJ_RS01915 0.22 -0.4 366,938 - MMJJ_RS01915 0.38 -1.0 366,939 + MMJJ_RS01915 0.38 -1.0 366,984 + MMJJ_RS01915 0.44 -1.3 367,015 + MMJJ_RS01915 0.48 -0.0 367,047 + MMJJ_RS01915 0.53 -1.2 367,087 - MMJJ_RS01915 0.58 -0.6 367,116 - MMJJ_RS01915 0.62 -1.3 367,150 + MMJJ_RS01915 0.67 -0.5 367,212 - MMJJ_RS01915 0.75 -1.3 367,215 - MMJJ_RS01915 0.75 -1.0 367,225 - MMJJ_RS01915 0.77 -0.6 367,226 + MMJJ_RS01915 0.77 -0.7 367,236 - MMJJ_RS01915 0.78 -0.3 367,237 + MMJJ_RS01915 0.78 -1.4 367,237 + MMJJ_RS01915 0.78 -1.2 367,322 + MMJJ_RS01915 0.90 +0.4 367,323 - MMJJ_RS01915 0.90 -1.1 367,375 + -0.2 367,382 - +0.6 367,382 - +0.9 367,397 - +0.3 367,402 + +0.6 367,411 + -0.7 367,419 - +1.3 367,419 - +1.5 367,419 - -0.0 367,419 - +0.1 367,420 + +1.0 367,420 + +0.1 367,420 + +0.7 367,420 + +0.0 367,420 + +0.7 367,420 + -0.2 367,421 - -0.6 367,441 - -0.1 367,474 - +0.2 367,475 + +0.2 367,476 - +1.2 367,476 - -0.5 367,477 + +0.3 367,477 + -0.4 367,480 + +1.6 367,550 + MMJJ_RS01920 0.11 +1.8 367,577 - MMJJ_RS01920 0.13 +0.1 367,577 - MMJJ_RS01920 0.13 +0.0 367,608 - MMJJ_RS01920 0.16 +0.4 367,608 - MMJJ_RS01920 0.16 +0.4 367,678 + MMJJ_RS01920 0.21 +0.4 367,678 + MMJJ_RS01920 0.21 +1.5 367,681 + MMJJ_RS01920 0.22 +1.6 367,686 - MMJJ_RS01920 0.22 +0.4 367,686 - MMJJ_RS01920 0.22 +0.6 367,686 - MMJJ_RS01920 0.22 +0.2 367,686 - MMJJ_RS01920 0.22 +0.1 367,687 + MMJJ_RS01920 0.22 +0.0 367,695 - MMJJ_RS01920 0.23 +0.2 367,704 + MMJJ_RS01920 0.23 -0.3 367,734 - MMJJ_RS01920 0.26 -0.3 367,738 + MMJJ_RS01920 0.26 -0.6 367,798 - MMJJ_RS01920 0.31 +0.1 367,798 - MMJJ_RS01920 0.31 +1.1 367,798 - MMJJ_RS01920 0.31 -0.4 367,799 + MMJJ_RS01920 0.31 +0.1 367,799 + MMJJ_RS01920 0.31 +0.6 367,799 + MMJJ_RS01920 0.31 -0.1 367,799 + MMJJ_RS01920 0.31 +0.4 367,804 + MMJJ_RS01920 0.31 +0.3 367,804 + MMJJ_RS01920 0.31 +0.4 367,808 + MMJJ_RS01920 0.31 +0.4 367,810 - MMJJ_RS01920 0.32 +0.2 367,810 - MMJJ_RS01920 0.32 -1.0 367,811 + MMJJ_RS01920 0.32 +0.1 367,815 + MMJJ_RS01920 0.32 +1.1 367,863 + MMJJ_RS01920 0.36 +0.6 367,866 - MMJJ_RS01920 0.36 -0.5 367,866 - MMJJ_RS01920 0.36 -0.0 367,867 + MMJJ_RS01920 0.36 -0.5 367,867 + MMJJ_RS01920 0.36 -0.4 367,870 - MMJJ_RS01920 0.36 -0.2 367,881 - MMJJ_RS01920 0.37 -0.3 367,910 - MMJJ_RS01920 0.39 -1.2 367,936 + MMJJ_RS01920 0.41 +0.3 367,943 + MMJJ_RS01920 0.42 +0.1 367,965 + MMJJ_RS01920 0.44 +0.1 368,016 + MMJJ_RS01920 0.48 -0.2 368,016 + MMJJ_RS01920 0.48 -1.1 368,037 + MMJJ_RS01920 0.49 +0.4 368,117 - MMJJ_RS01920 0.55 +0.7 368,117 - MMJJ_RS01920 0.55 +0.3 368,118 + MMJJ_RS01920 0.55 +0.2 368,139 - MMJJ_RS01920 0.57 -0.1 368,140 + MMJJ_RS01920 0.57 +0.3 368,140 + MMJJ_RS01920 0.57 -0.2 368,167 + -0.7 368,167 + MMJJ_RS01920 0.59 +0.5 368,228 - MMJJ_RS01920 0.64 +0.2 368,307 + MMJJ_RS01920 0.70 -1.4 368,333 - MMJJ_RS01920 0.72 -1.6 368,333 - MMJJ_RS01920 0.72 +0.5 368,357 - MMJJ_RS01920 0.74 -0.5 368,362 + MMJJ_RS01920 0.74 -0.3 368,362 + MMJJ_RS01920 0.74 +0.4 368,377 - MMJJ_RS01920 0.76 +0.5 368,378 + MMJJ_RS01920 0.76 +0.5 368,378 + MMJJ_RS01920 0.76 +1.2 368,378 + MMJJ_RS01920 0.76 -0.6 368,378 + MMJJ_RS01920 0.76 -0.1 368,388 - MMJJ_RS01920 0.76 +0.5 368,416 - MMJJ_RS01920 0.79 +0.5 368,417 + MMJJ_RS01920 0.79 +0.5 368,432 - MMJJ_RS01920 0.80 -0.4 368,470 + +2.5 368,470 + MMJJ_RS01920 0.83 +0.9 368,511 - MMJJ_RS01920 0.86 -0.2 368,556 - MMJJ_RS01920 0.90 +1.4 368,617 + +0.0 368,704 + +1.6 368,707 - +0.8 368,708 + +0.0 368,708 + -0.3 368,711 + +0.4 368,719 - -0.3 368,720 + +0.0 368,720 + -1.4 368,729 + -0.0 368,729 + +1.1 368,729 + -0.4 368,736 - -0.0 368,737 + +0.1 368,740 + -0.4 368,748 - -0.6 368,750 - -0.0 368,750 - -0.2 368,751 + -0.6 368,751 + -0.2 368,751 + -0.3 368,752 - +1.4 368,752 - +1.0 368,752 - -0.7 368,753 + -0.3 368,758 - +0.9 368,765 + -0.3 368,784 - +0.1 368,784 - -1.0 368,785 + +1.4 368,786 - +1.3 368,787 + +0.2 368,787 + +0.0 368,788 - -0.3 368,788 - +0.8 368,788 - +0.1 368,788 - +1.0 368,789 + -0.8 368,789 + +0.2 368,807 - +0.6 368,823 + +0.5 368,828 - -0.2 368,836 - +0.4 368,836 - +0.3 368,840 + -0.9 368,842 + +0.1 368,849 - +0.0 368,849 - -0.3 368,985 - MMJJ_RS01925 0.20 -0.4 369,010 + MMJJ_RS01925 0.23 -1.0 369,237 + MMJJ_RS01925 0.56 +0.0 369,258 - MMJJ_RS01925 0.59 -0.2 369,280 + MMJJ_RS01925 0.62 -0.8 369,388 - MMJJ_RS01925 0.78 -0.5 369,393 - MMJJ_RS01925 0.79 +0.0 369,395 - MMJJ_RS01925 0.79 -1.2 369,395 - MMJJ_RS01925 0.79 -0.6 369,400 - MMJJ_RS01925 0.80 -1.2 369,400 - MMJJ_RS01925 0.80 -0.7 369,400 - MMJJ_RS01925 0.80 -0.0 369,400 - MMJJ_RS01925 0.80 -0.4 369,401 + MMJJ_RS01925 0.80 -0.9 369,402 - MMJJ_RS01925 0.80 -0.9 369,414 + MMJJ_RS01925 0.82 +0.7 369,414 + MMJJ_RS01925 0.82 +0.9 369,415 - MMJJ_RS01925 0.82 -0.6 369,415 - MMJJ_RS01925 0.82 -0.5 369,418 - MMJJ_RS01925 0.82 -0.8 369,451 - MMJJ_RS01925 0.87 +0.5 369,475 + -0.1 369,475 + -0.4 369,502 - -1.2 369,502 - -0.9 369,502 - +0.1 369,502 - -0.8 369,502 - -0.7 369,503 + -0.9 369,503 + -0.7 369,503 + -0.7 369,503 + -1.1 369,503 + +0.0 369,559 - -0.5 369,560 + -0.4
Or see this region's nucleotide sequence