Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23025 and CA265_RS23030 are separated by 388 nucleotides CA265_RS23030 and CA265_RS23035 are separated by 59 nucleotides CA265_RS23035 and CA265_RS23040 are separated by 332 nucleotides
CA265_RS23025: CA265_RS23025 - diaminopimelate epimerase, at 5,639,103 to 5,639,885
_RS23025
CA265_RS23030: CA265_RS23030 - serine protease, at 5,640,274 to 5,641,836
_RS23030
CA265_RS23035: CA265_RS23035 - four-helix bundle copper-binding protein, at 5,641,896 to 5,642,240
_RS23035
CA265_RS23040: CA265_RS23040 - hypothetical protein, at 5,642,573 to 5,643,076
_RS23040
Position (kb) 5640
5641
5642 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5639.928 kb on - strand at 5639.954 kb on - strand at 5640.005 kb on - strand at 5640.126 kb on + strand at 5640.146 kb on - strand at 5640.165 kb on - strand at 5640.190 kb on + strand at 5640.193 kb on - strand at 5640.194 kb on + strand at 5640.194 kb on + strand at 5640.195 kb on - strand at 5640.227 kb on + strand at 5640.250 kb on + strand at 5640.275 kb on + strand at 5640.277 kb on + strand at 5640.277 kb on + strand at 5640.477 kb on - strand, within CA265_RS23030 at 5640.650 kb on - strand, within CA265_RS23030 at 5640.650 kb on - strand, within CA265_RS23030 at 5640.792 kb on - strand, within CA265_RS23030 at 5640.797 kb on + strand, within CA265_RS23030 at 5640.874 kb on - strand, within CA265_RS23030 at 5641.208 kb on - strand, within CA265_RS23030 at 5641.490 kb on - strand, within CA265_RS23030 at 5641.496 kb on + strand, within CA265_RS23030 at 5641.571 kb on + strand at 5641.637 kb on + strand, within CA265_RS23030 at 5641.674 kb on + strand, within CA265_RS23030 at 5641.694 kb on + strand at 5641.754 kb on + strand at 5641.810 kb on + strand at 5641.816 kb on + strand at 5641.816 kb on + strand at 5641.817 kb on - strand at 5641.817 kb on - strand at 5641.817 kb on - strand at 5641.835 kb on - strand at 5641.853 kb on + strand at 5642.037 kb on + strand, within CA265_RS23035 at 5642.042 kb on - strand, within CA265_RS23035 at 5642.042 kb on - strand, within CA265_RS23035 at 5642.096 kb on + strand, within CA265_RS23035 at 5642.097 kb on - strand, within CA265_RS23035 at 5642.221 kb on + strand at 5642.222 kb on - strand at 5642.262 kb on - strand at 5642.274 kb on + strand at 5642.378 kb on - strand at 5642.386 kb on - strand at 5642.400 kb on + strand at 5642.442 kb on + strand at 5642.484 kb on - strand at 5642.572 kb on + strand at 5642.580 kb on + strand at 5642.580 kb on + strand at 5642.650 kb on - strand, within CA265_RS23040 at 5642.659 kb on + strand, within CA265_RS23040 at 5642.659 kb on + strand, within CA265_RS23040 at 5642.659 kb on + strand, within CA265_RS23040 at 5642.660 kb on - strand, within CA265_RS23040 at 5642.663 kb on + strand, within CA265_RS23040 at 5642.692 kb on - strand, within CA265_RS23040 at 5642.692 kb on - strand, within CA265_RS23040 at 5642.692 kb on - strand, within CA265_RS23040 at 5642.777 kb on + strand, within CA265_RS23040 at 5642.812 kb on + strand, within CA265_RS23040
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 5,639,928 - +0.2 5,639,954 - +1.1 5,640,005 - -1.4 5,640,126 + -0.7 5,640,146 - -0.1 5,640,165 - -1.3 5,640,190 + -3.1 5,640,193 - +1.1 5,640,194 + +0.5 5,640,194 + +0.2 5,640,195 - +1.3 5,640,227 + +0.7 5,640,250 + +0.3 5,640,275 + +1.5 5,640,277 + +0.0 5,640,277 + +0.4 5,640,477 - CA265_RS23030 0.13 -0.5 5,640,650 - CA265_RS23030 0.24 +0.0 5,640,650 - CA265_RS23030 0.24 -1.8 5,640,792 - CA265_RS23030 0.33 +1.3 5,640,797 + CA265_RS23030 0.33 -0.3 5,640,874 - CA265_RS23030 0.38 +2.3 5,641,208 - CA265_RS23030 0.60 -0.1 5,641,490 - CA265_RS23030 0.78 -0.7 5,641,496 + CA265_RS23030 0.78 +0.9 5,641,571 + +2.3 5,641,637 + CA265_RS23030 0.87 +0.7 5,641,674 + CA265_RS23030 0.90 -0.4 5,641,694 + +0.4 5,641,754 + +0.7 5,641,810 + +0.2 5,641,816 + +0.2 5,641,816 + -0.1 5,641,817 - +0.2 5,641,817 - -0.5 5,641,817 - +0.7 5,641,835 - -0.5 5,641,853 + +0.6 5,642,037 + CA265_RS23035 0.41 +0.0 5,642,042 - CA265_RS23035 0.42 +0.6 5,642,042 - CA265_RS23035 0.42 -0.8 5,642,096 + CA265_RS23035 0.58 -0.2 5,642,097 - CA265_RS23035 0.58 -0.4 5,642,221 + +0.2 5,642,222 - -2.2 5,642,262 - +0.3 5,642,274 + -1.1 5,642,378 - +0.8 5,642,386 - -0.8 5,642,400 + -1.5 5,642,442 + +0.0 5,642,484 - -0.3 5,642,572 + -0.3 5,642,580 + +0.7 5,642,580 + +0.3 5,642,650 - CA265_RS23040 0.15 -0.9 5,642,659 + CA265_RS23040 0.17 -2.0 5,642,659 + CA265_RS23040 0.17 +0.7 5,642,659 + CA265_RS23040 0.17 +0.2 5,642,660 - CA265_RS23040 0.17 +0.2 5,642,663 + CA265_RS23040 0.18 +0.6 5,642,692 - CA265_RS23040 0.24 -1.3 5,642,692 - CA265_RS23040 0.24 -0.7 5,642,692 - CA265_RS23040 0.24 -0.2 5,642,777 + CA265_RS23040 0.40 +0.2 5,642,812 + CA265_RS23040 0.47 -0.2
Or see this region's nucleotide sequence