Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14475 and CA265_RS14480 are separated by 6 nucleotides CA265_RS14480 and CA265_RS14485 are separated by 109 nucleotides CA265_RS14485 and CA265_RS14490 are separated by 173 nucleotides CA265_RS14490 and CA265_RS14495 are separated by 38 nucleotides
CA265_RS14475: CA265_RS14475 - glutathione peroxidase, at 3,650,485 to 3,650,997
_RS14475
CA265_RS14480: CA265_RS14480 - hypothetical protein, at 3,651,004 to 3,651,618
_RS14480
CA265_RS14485: CA265_RS14485 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 3,651,728 to 3,653,935
_RS14485
CA265_RS14490: CA265_RS14490 - transcriptional repressor, at 3,654,109 to 3,654,606
_RS14490
CA265_RS14495: CA265_RS14495 - adenylosuccinate synthase, at 3,654,645 to 3,655,925
_RS14495
Position (kb)
3651
3652
3653
3654 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3650.754 kb on + strand, within CA265_RS14475 at 3650.830 kb on - strand, within CA265_RS14475 at 3650.872 kb on - strand, within CA265_RS14475 at 3650.897 kb on - strand, within CA265_RS14475 at 3650.897 kb on - strand, within CA265_RS14475 at 3650.903 kb on - strand, within CA265_RS14475 at 3650.949 kb on + strand at 3650.972 kb on + strand at 3650.998 kb on + strand at 3651.000 kb on + strand at 3651.001 kb on - strand at 3651.001 kb on - strand at 3651.001 kb on - strand at 3651.005 kb on + strand at 3651.005 kb on + strand at 3651.006 kb on - strand at 3651.006 kb on - strand at 3651.049 kb on - strand at 3651.081 kb on + strand, within CA265_RS14480 at 3651.088 kb on + strand, within CA265_RS14480 at 3651.111 kb on - strand, within CA265_RS14480 at 3651.211 kb on - strand, within CA265_RS14480 at 3651.218 kb on - strand, within CA265_RS14480 at 3651.373 kb on + strand, within CA265_RS14480 at 3651.379 kb on - strand, within CA265_RS14480 at 3651.380 kb on + strand, within CA265_RS14480 at 3651.396 kb on - strand, within CA265_RS14480 at 3651.396 kb on - strand, within CA265_RS14480 at 3651.441 kb on + strand, within CA265_RS14480 at 3651.441 kb on + strand, within CA265_RS14480 at 3651.442 kb on - strand, within CA265_RS14480 at 3651.589 kb on - strand at 3651.654 kb on + strand at 3651.655 kb on - strand at 3651.677 kb on - strand at 3651.682 kb on - strand at 3651.695 kb on - strand at 3651.698 kb on + strand at 3651.699 kb on - strand at 3651.787 kb on + strand at 3651.814 kb on + strand at 3651.818 kb on + strand at 3651.883 kb on + strand at 3651.884 kb on - strand at 3651.908 kb on + strand at 3651.911 kb on - strand at 3651.922 kb on + strand at 3651.973 kb on + strand, within CA265_RS14485 at 3651.974 kb on - strand, within CA265_RS14485 at 3651.978 kb on + strand, within CA265_RS14485 at 3652.031 kb on - strand, within CA265_RS14485 at 3652.109 kb on - strand, within CA265_RS14485 at 3652.109 kb on - strand, within CA265_RS14485 at 3652.164 kb on + strand, within CA265_RS14485 at 3652.204 kb on + strand, within CA265_RS14485 at 3652.204 kb on + strand, within CA265_RS14485 at 3652.244 kb on - strand, within CA265_RS14485 at 3652.299 kb on + strand, within CA265_RS14485 at 3652.299 kb on + strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.340 kb on + strand, within CA265_RS14485 at 3652.359 kb on - strand, within CA265_RS14485 at 3652.620 kb on - strand, within CA265_RS14485 at 3652.630 kb on - strand, within CA265_RS14485 at 3652.639 kb on - strand, within CA265_RS14485 at 3652.749 kb on + strand, within CA265_RS14485 at 3652.760 kb on - strand, within CA265_RS14485 at 3653.075 kb on - strand, within CA265_RS14485 at 3653.075 kb on - strand, within CA265_RS14485 at 3653.934 kb on - strand at 3653.937 kb on + strand at 3653.972 kb on - strand at 3654.035 kb on + strand at 3654.035 kb on + strand at 3654.080 kb on + strand at 3654.084 kb on + strand at 3654.614 kb on + strand at 3654.614 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,650,754 + CA265_RS14475 0.52 -2.3 3,650,830 - CA265_RS14475 0.67 +1.1 3,650,872 - CA265_RS14475 0.75 -0.8 3,650,897 - CA265_RS14475 0.80 -0.1 3,650,897 - CA265_RS14475 0.80 -0.0 3,650,903 - CA265_RS14475 0.81 -1.1 3,650,949 + -0.4 3,650,972 + -0.0 3,650,998 + -0.2 3,651,000 + +0.2 3,651,001 - +0.3 3,651,001 - +0.5 3,651,001 - +0.3 3,651,005 + -0.8 3,651,005 + -0.4 3,651,006 - +1.4 3,651,006 - -0.5 3,651,049 - -0.7 3,651,081 + CA265_RS14480 0.13 -1.7 3,651,088 + CA265_RS14480 0.14 -1.0 3,651,111 - CA265_RS14480 0.17 -0.2 3,651,211 - CA265_RS14480 0.34 -0.8 3,651,218 - CA265_RS14480 0.35 -0.7 3,651,373 + CA265_RS14480 0.60 +1.0 3,651,379 - CA265_RS14480 0.61 +0.4 3,651,380 + CA265_RS14480 0.61 -1.5 3,651,396 - CA265_RS14480 0.64 -0.1 3,651,396 - CA265_RS14480 0.64 -0.5 3,651,441 + CA265_RS14480 0.71 -0.1 3,651,441 + CA265_RS14480 0.71 +1.4 3,651,442 - CA265_RS14480 0.71 -1.2 3,651,589 - +0.7 3,651,654 + +1.8 3,651,655 - -0.2 3,651,677 - -1.1 3,651,682 - +1.2 3,651,695 - -0.9 3,651,698 + +0.5 3,651,699 - -1.3 3,651,787 + -2.6 3,651,814 + -2.9 3,651,818 + -0.6 3,651,883 + -4.6 3,651,884 - -1.3 3,651,908 + -3.9 3,651,911 - -5.4 3,651,922 + -2.6 3,651,973 + CA265_RS14485 0.11 -4.1 3,651,974 - CA265_RS14485 0.11 -3.5 3,651,978 + CA265_RS14485 0.11 -1.1 3,652,031 - CA265_RS14485 0.14 -2.1 3,652,109 - CA265_RS14485 0.17 -3.1 3,652,109 - CA265_RS14485 0.17 -3.0 3,652,164 + CA265_RS14485 0.20 -0.8 3,652,204 + CA265_RS14485 0.22 -3.0 3,652,204 + CA265_RS14485 0.22 -3.3 3,652,244 - CA265_RS14485 0.23 -1.2 3,652,299 + CA265_RS14485 0.26 -3.7 3,652,299 + CA265_RS14485 0.26 -3.9 3,652,300 - CA265_RS14485 0.26 -3.5 3,652,300 - CA265_RS14485 0.26 -1.8 3,652,300 - CA265_RS14485 0.26 -4.8 3,652,300 - CA265_RS14485 0.26 -1.4 3,652,340 + CA265_RS14485 0.28 -1.0 3,652,359 - CA265_RS14485 0.29 -4.1 3,652,620 - CA265_RS14485 0.40 -2.4 3,652,630 - CA265_RS14485 0.41 -1.0 3,652,639 - CA265_RS14485 0.41 -4.4 3,652,749 + CA265_RS14485 0.46 -2.7 3,652,760 - CA265_RS14485 0.47 -0.6 3,653,075 - CA265_RS14485 0.61 -3.7 3,653,075 - CA265_RS14485 0.61 -3.3 3,653,934 - -0.8 3,653,937 + +0.4 3,653,972 - +0.5 3,654,035 + +0.3 3,654,035 + -0.7 3,654,080 + +0.2 3,654,084 + +0.4 3,654,614 + +0.9 3,654,614 + -0.0
Or see this region's nucleotide sequence