Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04105 and CA265_RS04110 are separated by 2 nucleotides CA265_RS04110 and CA265_RS04115 are separated by 193 nucleotides CA265_RS04115 and CA265_RS04120 are separated by 120 nucleotides
CA265_RS04105: CA265_RS04105 - siroheme synthase, at 988,096 to 989,607
_RS04105
CA265_RS04110: CA265_RS04110 - phosphoadenosine phosphosulfate reductase, at 989,610 to 990,308
_RS04110
CA265_RS04115: CA265_RS04115 - sulfate adenylyltransferase, at 990,502 to 991,407
_RS04115
CA265_RS04120: CA265_RS04120 - sulfate adenylyltransferase, at 991,528 to 992,778
_RS04120
Position (kb)
990
991
992 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 989.565 kb on - strand at 989.655 kb on - strand at 989.685 kb on - strand, within CA265_RS04110 at 989.766 kb on - strand, within CA265_RS04110 at 989.775 kb on - strand, within CA265_RS04110 at 989.818 kb on - strand, within CA265_RS04110 at 989.840 kb on - strand, within CA265_RS04110 at 989.840 kb on - strand, within CA265_RS04110 at 989.848 kb on - strand, within CA265_RS04110 at 989.863 kb on - strand, within CA265_RS04110 at 989.863 kb on - strand, within CA265_RS04110 at 989.962 kb on - strand, within CA265_RS04110 at 989.962 kb on - strand, within CA265_RS04110 at 989.996 kb on - strand, within CA265_RS04110 at 990.057 kb on - strand, within CA265_RS04110 at 990.108 kb on - strand, within CA265_RS04110 at 990.125 kb on - strand, within CA265_RS04110 at 990.166 kb on - strand, within CA265_RS04110 at 990.198 kb on - strand, within CA265_RS04110 at 990.306 kb on + strand at 990.307 kb on - strand at 990.307 kb on - strand at 990.307 kb on - strand at 990.433 kb on + strand at 990.434 kb on - strand at 990.461 kb on - strand at 990.507 kb on + strand at 990.507 kb on + strand at 990.513 kb on + strand at 990.514 kb on - strand at 990.693 kb on + strand, within CA265_RS04115 at 990.721 kb on - strand, within CA265_RS04115 at 990.725 kb on - strand, within CA265_RS04115 at 990.789 kb on - strand, within CA265_RS04115 at 990.877 kb on + strand, within CA265_RS04115 at 990.877 kb on + strand, within CA265_RS04115 at 991.013 kb on - strand, within CA265_RS04115 at 991.090 kb on + strand, within CA265_RS04115 at 991.190 kb on + strand, within CA265_RS04115 at 991.190 kb on + strand, within CA265_RS04115 at 991.194 kb on + strand, within CA265_RS04115 at 991.252 kb on - strand, within CA265_RS04115 at 991.303 kb on - strand, within CA265_RS04115 at 991.303 kb on - strand, within CA265_RS04115 at 991.495 kb on - strand at 991.536 kb on - strand at 991.572 kb on + strand at 991.606 kb on - strand at 991.606 kb on - strand at 991.676 kb on + strand, within CA265_RS04120 at 991.739 kb on + strand, within CA265_RS04120 at 991.776 kb on + strand, within CA265_RS04120 at 991.776 kb on + strand, within CA265_RS04120 at 991.789 kb on - strand, within CA265_RS04120 at 991.808 kb on - strand, within CA265_RS04120 at 991.809 kb on + strand, within CA265_RS04120 at 991.810 kb on - strand, within CA265_RS04120 at 991.906 kb on + strand, within CA265_RS04120 at 991.907 kb on - strand at 991.962 kb on + strand, within CA265_RS04120 at 991.972 kb on + strand, within CA265_RS04120 at 991.987 kb on + strand, within CA265_RS04120 at 992.051 kb on - strand, within CA265_RS04120 at 992.113 kb on + strand, within CA265_RS04120 at 992.114 kb on - strand, within CA265_RS04120 at 992.141 kb on + strand, within CA265_RS04120 at 992.170 kb on - strand, within CA265_RS04120 at 992.170 kb on - strand, within CA265_RS04120 at 992.196 kb on - strand, within CA265_RS04120 at 992.229 kb on - strand, within CA265_RS04120 at 992.258 kb on + strand, within CA265_RS04120 at 992.259 kb on - strand, within CA265_RS04120 at 992.298 kb on - strand, within CA265_RS04120 at 992.385 kb on + strand, within CA265_RS04120 at 992.385 kb on + strand, within CA265_RS04120
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 989,565 - -2.1 989,655 - -0.9 989,685 - CA265_RS04110 0.11 -1.8 989,766 - CA265_RS04110 0.22 -2.7 989,775 - CA265_RS04110 0.24 -4.7 989,818 - CA265_RS04110 0.30 -4.2 989,840 - CA265_RS04110 0.33 -4.5 989,840 - CA265_RS04110 0.33 -1.4 989,848 - CA265_RS04110 0.34 -4.4 989,863 - CA265_RS04110 0.36 -3.6 989,863 - CA265_RS04110 0.36 -4.6 989,962 - CA265_RS04110 0.50 -3.4 989,962 - CA265_RS04110 0.50 -1.5 989,996 - CA265_RS04110 0.55 -1.2 990,057 - CA265_RS04110 0.64 -1.5 990,108 - CA265_RS04110 0.71 -1.3 990,125 - CA265_RS04110 0.74 -3.1 990,166 - CA265_RS04110 0.80 -2.3 990,198 - CA265_RS04110 0.84 -4.3 990,306 + -0.2 990,307 - -2.1 990,307 - -1.5 990,307 - -2.8 990,433 + +0.6 990,434 - -3.1 990,461 - -2.9 990,507 + -1.4 990,507 + -2.4 990,513 + +0.4 990,514 - -3.4 990,693 + CA265_RS04115 0.21 -2.8 990,721 - CA265_RS04115 0.24 -0.8 990,725 - CA265_RS04115 0.25 -2.1 990,789 - CA265_RS04115 0.32 -0.2 990,877 + CA265_RS04115 0.41 -2.0 990,877 + CA265_RS04115 0.41 -1.8 991,013 - CA265_RS04115 0.56 -2.0 991,090 + CA265_RS04115 0.65 -2.4 991,190 + CA265_RS04115 0.76 -3.7 991,190 + CA265_RS04115 0.76 -3.1 991,194 + CA265_RS04115 0.76 -2.8 991,252 - CA265_RS04115 0.83 -2.0 991,303 - CA265_RS04115 0.88 -3.2 991,303 - CA265_RS04115 0.88 -3.0 991,495 - -1.6 991,536 - -0.5 991,572 + -3.8 991,606 - -3.6 991,606 - -2.0 991,676 + CA265_RS04120 0.12 -1.4 991,739 + CA265_RS04120 0.17 -2.8 991,776 + CA265_RS04120 0.20 -3.2 991,776 + CA265_RS04120 0.20 -3.7 991,789 - CA265_RS04120 0.21 -2.9 991,808 - CA265_RS04120 0.22 -1.8 991,809 + CA265_RS04120 0.22 -2.4 991,810 - CA265_RS04120 0.23 -3.2 991,906 + CA265_RS04120 0.30 -1.3 991,907 - +0.4 991,962 + CA265_RS04120 0.35 -1.4 991,972 + CA265_RS04120 0.35 -2.7 991,987 + CA265_RS04120 0.37 -1.9 992,051 - CA265_RS04120 0.42 -4.8 992,113 + CA265_RS04120 0.47 -2.4 992,114 - CA265_RS04120 0.47 -4.6 992,141 + CA265_RS04120 0.49 -3.3 992,170 - CA265_RS04120 0.51 -4.0 992,170 - CA265_RS04120 0.51 -4.5 992,196 - CA265_RS04120 0.53 -0.9 992,229 - CA265_RS04120 0.56 -3.1 992,258 + CA265_RS04120 0.58 -1.9 992,259 - CA265_RS04120 0.58 -3.5 992,298 - CA265_RS04120 0.62 -3.1 992,385 + CA265_RS04120 0.69 -3.3 992,385 + CA265_RS04120 0.69 -4.1
Or see this region's nucleotide sequence