Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28190

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28185 and BPHYT_RS28190 are separated by 50 nucleotidesBPHYT_RS28190 and BPHYT_RS28195 are separated by 243 nucleotidesBPHYT_RS28195 and BPHYT_RS28200 are separated by 101 nucleotides BPHYT_RS28185: BPHYT_RS28185 - lipase, at 1,882,077 to 1,883,144 _RS28185 BPHYT_RS28190: BPHYT_RS28190 - porin, at 1,883,195 to 1,884,280 _RS28190 BPHYT_RS28195: BPHYT_RS28195 - hypothetical protein, at 1,884,524 to 1,884,850 _RS28195 BPHYT_RS28200: BPHYT_RS28200 - sugar ABC transporter substrate-binding protein, at 1,884,952 to 1,885,959 _RS28200 Position (kb) 1883 1884 1885Strain fitness (log2 ratio) -1 0 1at 1882.344 kb on + strand, within BPHYT_RS28185at 1882.345 kb on - strand, within BPHYT_RS28185at 1882.779 kb on + strand, within BPHYT_RS28185at 1883.025 kb on + strand, within BPHYT_RS28185at 1883.160 kb on + strandat 1883.291 kb on + strandat 1883.894 kb on + strand, within BPHYT_RS28190at 1883.925 kb on - strand, within BPHYT_RS28190at 1885.251 kb on + strand, within BPHYT_RS28200

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,882,344 + BPHYT_RS28185 0.25 -0.0
1,882,345 - BPHYT_RS28185 0.25 +0.3
1,882,779 + BPHYT_RS28185 0.66 -0.5
1,883,025 + BPHYT_RS28185 0.89 +0.8
1,883,160 + +0.1
1,883,291 + -0.2
1,883,894 + BPHYT_RS28190 0.64 +1.3
1,883,925 - BPHYT_RS28190 0.67 +0.5
1,885,251 + BPHYT_RS28200 0.30 -0.3

Or see this region's nucleotide sequence