Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27520

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27510 and BPHYT_RS27515 are separated by 83 nucleotidesBPHYT_RS27515 and BPHYT_RS27520 are separated by 688 nucleotidesBPHYT_RS27520 and BPHYT_RS27525 are separated by 220 nucleotidesBPHYT_RS27525 and BPHYT_RS27530 are separated by 74 nucleotides BPHYT_RS27510: BPHYT_RS27510 - hypothetical protein, at 1,747,928 to 1,748,290 _RS27510 BPHYT_RS27515: BPHYT_RS27515 - hypothetical protein, at 1,748,374 to 1,748,565 _RS27515 BPHYT_RS27520: BPHYT_RS27520 - membrane protein, at 1,749,254 to 1,749,913 _RS27520 BPHYT_RS27525: BPHYT_RS27525 - lipocalin, at 1,750,134 to 1,750,703 _RS27525 BPHYT_RS27530: BPHYT_RS27530 - transport-associated protein, at 1,750,778 to 1,751,428 _RS27530 Position (kb) 1749 1750Strain fitness (log2 ratio) -1 0 1at 1749.346 kb on - strand, within BPHYT_RS27520at 1749.365 kb on + strand, within BPHYT_RS27520at 1749.366 kb on - strand, within BPHYT_RS27520at 1749.366 kb on - strand, within BPHYT_RS27520at 1750.020 kb on + strandat 1750.020 kb on + strandat 1750.131 kb on - strandat 1750.273 kb on - strand, within BPHYT_RS27525at 1750.323 kb on + strand, within BPHYT_RS27525at 1750.368 kb on + strand, within BPHYT_RS27525at 1750.368 kb on + strand, within BPHYT_RS27525

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,749,346 - BPHYT_RS27520 0.14 +1.1
1,749,365 + BPHYT_RS27520 0.17 +0.5
1,749,366 - BPHYT_RS27520 0.17 +0.3
1,749,366 - BPHYT_RS27520 0.17 +0.6
1,750,020 + -1.3
1,750,020 + +0.1
1,750,131 - -1.6
1,750,273 - BPHYT_RS27525 0.24 -0.0
1,750,323 + BPHYT_RS27525 0.33 +0.3
1,750,368 + BPHYT_RS27525 0.41 -0.1
1,750,368 + BPHYT_RS27525 0.41 -0.1

Or see this region's nucleotide sequence