Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27510 and BPHYT_RS27515 are separated by 83 nucleotides BPHYT_RS27515 and BPHYT_RS27520 are separated by 688 nucleotides BPHYT_RS27520 and BPHYT_RS27525 are separated by 220 nucleotides BPHYT_RS27525 and BPHYT_RS27530 are separated by 74 nucleotides
BPHYT_RS27510: BPHYT_RS27510 - hypothetical protein, at 1,747,928 to 1,748,290
_RS27510
BPHYT_RS27515: BPHYT_RS27515 - hypothetical protein, at 1,748,374 to 1,748,565
_RS27515
BPHYT_RS27520: BPHYT_RS27520 - membrane protein, at 1,749,254 to 1,749,913
_RS27520
BPHYT_RS27525: BPHYT_RS27525 - lipocalin, at 1,750,134 to 1,750,703
_RS27525
BPHYT_RS27530: BPHYT_RS27530 - transport-associated protein, at 1,750,778 to 1,751,428
_RS27530
Position (kb)
1749
1750 Strain fitness (log2 ratio)
-1
0
1 at 1749.346 kb on - strand, within BPHYT_RS27520 at 1749.365 kb on + strand, within BPHYT_RS27520 at 1749.366 kb on - strand, within BPHYT_RS27520 at 1749.366 kb on - strand, within BPHYT_RS27520 at 1750.020 kb on + strand at 1750.020 kb on + strand at 1750.131 kb on - strand at 1750.273 kb on - strand, within BPHYT_RS27525 at 1750.323 kb on + strand, within BPHYT_RS27525 at 1750.368 kb on + strand, within BPHYT_RS27525 at 1750.368 kb on + strand, within BPHYT_RS27525
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,749,346 - BPHYT_RS27520 0.14 +1.1 1,749,365 + BPHYT_RS27520 0.17 +0.5 1,749,366 - BPHYT_RS27520 0.17 +0.3 1,749,366 - BPHYT_RS27520 0.17 +0.6 1,750,020 + -1.3 1,750,020 + +0.1 1,750,131 - -1.6 1,750,273 - BPHYT_RS27525 0.24 -0.0 1,750,323 + BPHYT_RS27525 0.33 +0.3 1,750,368 + BPHYT_RS27525 0.41 -0.1 1,750,368 + BPHYT_RS27525 0.41 -0.1
Or see this region's nucleotide sequence