Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24730

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24725 and BPHYT_RS24730 are separated by 207 nucleotidesBPHYT_RS24730 and BPHYT_RS24735 are separated by 93 nucleotidesBPHYT_RS24735 and BPHYT_RS24740 overlap by 4 nucleotides BPHYT_RS24725: BPHYT_RS24725 - NmrA family transcriptional regulator, at 1,113,598 to 1,114,521 _RS24725 BPHYT_RS24730: BPHYT_RS24730 - AraC family transcriptional regulator, at 1,114,729 to 1,115,685 _RS24730 BPHYT_RS24735: BPHYT_RS24735 - transcriptional regulator, at 1,115,779 to 1,116,606 _RS24735 BPHYT_RS24740: BPHYT_RS24740 - RNA polymerase subunit sigma-24, at 1,116,603 to 1,117,121 _RS24740 Position (kb) 1114 1115 1116Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1113.911 kb on - strand, within BPHYT_RS24725at 1114.066 kb on + strand, within BPHYT_RS24725at 1114.067 kb on - strand, within BPHYT_RS24725at 1114.086 kb on + strand, within BPHYT_RS24725at 1114.087 kb on - strand, within BPHYT_RS24725at 1114.558 kb on + strandat 1114.624 kb on + strandat 1114.656 kb on - strandat 1114.659 kb on + strandat 1114.884 kb on + strand, within BPHYT_RS24730at 1114.885 kb on - strand, within BPHYT_RS24730at 1114.900 kb on + strand, within BPHYT_RS24730at 1115.242 kb on - strand, within BPHYT_RS24730at 1115.634 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,113,911 - BPHYT_RS24725 0.34 +0.4
1,114,066 + BPHYT_RS24725 0.51 +0.8
1,114,067 - BPHYT_RS24725 0.51 -1.0
1,114,086 + BPHYT_RS24725 0.53 -2.1
1,114,087 - BPHYT_RS24725 0.53 -0.3
1,114,558 + +2.8
1,114,624 + -1.2
1,114,656 - +0.4
1,114,659 + -0.5
1,114,884 + BPHYT_RS24730 0.16 +1.6
1,114,885 - BPHYT_RS24730 0.16 -0.0
1,114,900 + BPHYT_RS24730 0.18 +0.9
1,115,242 - BPHYT_RS24730 0.54 -0.0
1,115,634 + -0.0

Or see this region's nucleotide sequence