Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24000 and BPHYT_RS24005 are separated by 102 nucleotides BPHYT_RS24005 and BPHYT_RS24010 are separated by 14 nucleotides BPHYT_RS24010 and BPHYT_RS24015 are separated by 35 nucleotides BPHYT_RS24015 and BPHYT_RS24020 are separated by 38 nucleotides
BPHYT_RS24000: BPHYT_RS24000 - L-histidine ABC transporter, substrate-binding component <small>(from data)</small>, at 950,479 to 951,339
_RS24000
BPHYT_RS24005: BPHYT_RS24005 - L-histidine ABC transporter, permease component 1 <small>(from data)</small>, at 951,442 to 952,164
_RS24005
BPHYT_RS24010: BPHYT_RS24010 - L-histidine ABC transporter, permease component 2 <small>(from data)</small>, at 952,179 to 952,931
_RS24010
BPHYT_RS24015: BPHYT_RS24015 - L-histidine ABC transporter, ATPase component <small>(from data)</small>, at 952,967 to 953,758
_RS24015
BPHYT_RS24020: BPHYT_RS24020 - phenylalanine/histidine ammonia-lyase, at 953,797 to 955,446
_RS24020
Position (kb) 952
953 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 951.436 kb on + strand at 951.491 kb on + strand at 951.622 kb on + strand, within BPHYT_RS24005 at 951.622 kb on + strand, within BPHYT_RS24005 at 951.791 kb on - strand, within BPHYT_RS24005 at 952.039 kb on + strand, within BPHYT_RS24005 at 952.467 kb on + strand, within BPHYT_RS24010 at 952.467 kb on + strand, within BPHYT_RS24010 at 952.791 kb on + strand, within BPHYT_RS24010
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 951,436 + -2.9 951,491 + -0.7 951,622 + BPHYT_RS24005 0.25 +0.5 951,622 + BPHYT_RS24005 0.25 -1.5 951,791 - BPHYT_RS24005 0.48 +0.4 952,039 + BPHYT_RS24005 0.83 +0.1 952,467 + BPHYT_RS24010 0.38 +0.5 952,467 + BPHYT_RS24010 0.38 +1.1 952,791 + BPHYT_RS24010 0.81 +0.7
Or see this region's nucleotide sequence