Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22315 and BPHYT_RS22320 are separated by 140 nucleotides BPHYT_RS22320 and BPHYT_RS22325 are separated by 102 nucleotides BPHYT_RS22325 and BPHYT_RS22330 overlap by 7 nucleotides BPHYT_RS22330 and BPHYT_RS22335 are separated by 266 nucleotides
BPHYT_RS22315: BPHYT_RS22315 - hypothetical protein, at 560,457 to 560,654
_RS22315
BPHYT_RS22320: BPHYT_RS22320 - hypothetical protein, at 560,795 to 561,532
_RS22320
BPHYT_RS22325: BPHYT_RS22325 - histidine kinase, at 561,635 to 563,134
_RS22325
BPHYT_RS22330: BPHYT_RS22330 - PhoB family transcriptional regulator, at 563,128 to 563,865
_RS22330
BPHYT_RS22335: BPHYT_RS22335 - cytochrome C biogenesis protein, at 564,132 to 566,012
_RS22335
Position (kb) 561
562
563
564 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 560.748 kb on + strand at 561.161 kb on - strand, within BPHYT_RS22320 at 561.413 kb on - strand, within BPHYT_RS22320 at 561.413 kb on - strand, within BPHYT_RS22320 at 561.440 kb on + strand, within BPHYT_RS22320 at 561.440 kb on + strand, within BPHYT_RS22320 at 561.555 kb on + strand at 561.728 kb on + strand at 562.313 kb on - strand, within BPHYT_RS22325 at 562.669 kb on - strand, within BPHYT_RS22325 at 562.675 kb on - strand, within BPHYT_RS22325 at 562.853 kb on + strand, within BPHYT_RS22325 at 562.854 kb on - strand, within BPHYT_RS22325 at 563.012 kb on + strand at 563.134 kb on + strand at 563.256 kb on + strand, within BPHYT_RS22330 at 563.256 kb on + strand, within BPHYT_RS22330 at 563.257 kb on - strand, within BPHYT_RS22330 at 563.357 kb on + strand, within BPHYT_RS22330 at 563.981 kb on + strand at 564.022 kb on + strand at 564.110 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 560,748 + +0.3 561,161 - BPHYT_RS22320 0.50 -1.1 561,413 - BPHYT_RS22320 0.84 +3.3 561,413 - BPHYT_RS22320 0.84 -0.0 561,440 + BPHYT_RS22320 0.87 +0.5 561,440 + BPHYT_RS22320 0.87 +1.1 561,555 + -0.4 561,728 + +0.2 562,313 - BPHYT_RS22325 0.45 +1.9 562,669 - BPHYT_RS22325 0.69 -0.0 562,675 - BPHYT_RS22325 0.69 -0.6 562,853 + BPHYT_RS22325 0.81 +0.2 562,854 - BPHYT_RS22325 0.81 +2.7 563,012 + +0.1 563,134 + -0.8 563,256 + BPHYT_RS22330 0.17 -0.4 563,256 + BPHYT_RS22330 0.17 +0.2 563,257 - BPHYT_RS22330 0.17 +0.4 563,357 + BPHYT_RS22330 0.31 -1.5 563,981 + -0.1 564,022 + -2.3 564,110 + -1.2
Or see this region's nucleotide sequence