Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20780

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20770 and BPHYT_RS20775 are separated by 7 nucleotidesBPHYT_RS20775 and BPHYT_RS20780 overlap by 4 nucleotidesBPHYT_RS20780 and BPHYT_RS20785 are separated by 213 nucleotides BPHYT_RS20770: BPHYT_RS20770 - hypothetical protein, at 221,214 to 222,383 _RS20770 BPHYT_RS20775: BPHYT_RS20775 - 3-ketoacyl-ACP reductase, at 222,391 to 223,170 _RS20775 BPHYT_RS20780: BPHYT_RS20780 - acyl-CoA dehydrogenase, at 223,167 to 224,312 _RS20780 BPHYT_RS20785: BPHYT_RS20785 - CoA-transferase, at 224,526 to 226,013 _RS20785 Position (kb) 223 224 225Strain fitness (log2 ratio) -1 0 1at 222.306 kb on + strandat 222.306 kb on + strandat 222.396 kb on - strandat 222.683 kb on + strand, within BPHYT_RS20775at 222.684 kb on - strand, within BPHYT_RS20775at 222.887 kb on - strand, within BPHYT_RS20775at 223.208 kb on + strandat 223.954 kb on + strand, within BPHYT_RS20780at 223.993 kb on + strand, within BPHYT_RS20780at 224.194 kb on + strand, within BPHYT_RS20780at 225.105 kb on + strand, within BPHYT_RS20785at 225.180 kb on + strand, within BPHYT_RS20785at 225.180 kb on + strand, within BPHYT_RS20785at 225.181 kb on - strand, within BPHYT_RS20785at 225.181 kb on - strand, within BPHYT_RS20785

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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222,306 + -0.3
222,306 + -0.2
222,396 - -0.0
222,683 + BPHYT_RS20775 0.37 -0.4
222,684 - BPHYT_RS20775 0.38 +0.3
222,887 - BPHYT_RS20775 0.64 +1.0
223,208 + -1.0
223,954 + BPHYT_RS20780 0.69 +0.7
223,993 + BPHYT_RS20780 0.72 +0.5
224,194 + BPHYT_RS20780 0.90 +0.5
225,105 + BPHYT_RS20785 0.39 -0.4
225,180 + BPHYT_RS20785 0.44 +0.9
225,180 + BPHYT_RS20785 0.44 +0.8
225,181 - BPHYT_RS20785 0.44 +0.2
225,181 - BPHYT_RS20785 0.44 -0.5

Or see this region's nucleotide sequence