Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS19090

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS19085 and BPHYT_RS19090 are separated by 157 nucleotidesBPHYT_RS19090 and BPHYT_RS19095 are separated by 3 nucleotidesBPHYT_RS19095 and BPHYT_RS19100 are separated by 263 nucleotides BPHYT_RS19085: BPHYT_RS19085 - flagellin, at 4,282,243 to 4,283,388 _RS19085 BPHYT_RS19090: BPHYT_RS19090 - flagellar hook protein FliD, at 4,283,546 to 4,285,063 _RS19090 BPHYT_RS19095: BPHYT_RS19095 - hypothetical protein, at 4,285,067 to 4,285,381 _RS19095 BPHYT_RS19100: BPHYT_RS19100 - hypothetical protein, at 4,285,645 to 4,288,017 _RS19100 Position (kb) 4283 4284 4285 4286Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4282.633 kb on + strand, within BPHYT_RS19085at 4282.819 kb on + strand, within BPHYT_RS19085at 4282.819 kb on + strand, within BPHYT_RS19085at 4282.842 kb on + strand, within BPHYT_RS19085at 4282.879 kb on + strand, within BPHYT_RS19085at 4282.880 kb on - strand, within BPHYT_RS19085at 4283.160 kb on + strand, within BPHYT_RS19085at 4283.160 kb on + strand, within BPHYT_RS19085at 4283.160 kb on + strand, within BPHYT_RS19085at 4283.161 kb on - strand, within BPHYT_RS19085at 4283.161 kb on - strand, within BPHYT_RS19085at 4283.488 kb on + strandat 4283.517 kb on - strandat 4283.894 kb on + strand, within BPHYT_RS19090at 4284.262 kb on + strandat 4284.262 kb on + strand, within BPHYT_RS19090at 4284.263 kb on - strand, within BPHYT_RS19090at 4284.263 kb on - strand, within BPHYT_RS19090at 4284.272 kb on - strand, within BPHYT_RS19090at 4284.320 kb on + strand, within BPHYT_RS19090at 4284.917 kb on + strandat 4284.918 kb on - strandat 4284.942 kb on - strandat 4285.061 kb on + strandat 4285.061 kb on + strandat 4285.061 kb on + strandat 4285.062 kb on - strandat 4285.358 kb on + strandat 4285.453 kb on + strandat 4285.453 kb on + strandat 4285.460 kb on + strandat 4285.461 kb on - strandat 4285.623 kb on + strandat 4285.623 kb on + strandat 4285.623 kb on + strandat 4285.624 kb on - strandat 4285.657 kb on + strandat 4285.825 kb on + strandat 4285.826 kb on - strandat 4285.826 kb on - strandat 4285.933 kb on + strand, within BPHYT_RS19100at 4285.933 kb on + strand, within BPHYT_RS19100at 4285.934 kb on - strand, within BPHYT_RS19100at 4285.934 kb on - strand, within BPHYT_RS19100

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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4,282,633 + BPHYT_RS19085 0.34 -2.9
4,282,819 + BPHYT_RS19085 0.50 -1.6
4,282,819 + BPHYT_RS19085 0.50 -4.4
4,282,842 + BPHYT_RS19085 0.52 -2.5
4,282,879 + BPHYT_RS19085 0.55 -4.0
4,282,880 - BPHYT_RS19085 0.56 -4.8
4,283,160 + BPHYT_RS19085 0.80 -2.1
4,283,160 + BPHYT_RS19085 0.80 -5.8
4,283,160 + BPHYT_RS19085 0.80 -2.1
4,283,161 - BPHYT_RS19085 0.80 -4.5
4,283,161 - BPHYT_RS19085 0.80 -4.4
4,283,488 + -0.3
4,283,517 - -2.3
4,283,894 + BPHYT_RS19090 0.23 -2.9
4,284,262 + +0.1
4,284,262 + BPHYT_RS19090 0.47 -0.7
4,284,263 - BPHYT_RS19090 0.47 -1.6
4,284,263 - BPHYT_RS19090 0.47 -3.5
4,284,272 - BPHYT_RS19090 0.48 +0.5
4,284,320 + BPHYT_RS19090 0.51 -0.2
4,284,917 + -1.0
4,284,918 - -2.8
4,284,942 - -1.8
4,285,061 + +0.3
4,285,061 + +0.4
4,285,061 + +0.4
4,285,062 - -1.0
4,285,358 + -0.3
4,285,453 + +0.4
4,285,453 + +0.7
4,285,460 + -0.1
4,285,461 - -1.0
4,285,623 + -0.2
4,285,623 + +0.4
4,285,623 + +0.6
4,285,624 - -2.3
4,285,657 + -1.8
4,285,825 + -2.4
4,285,826 - -2.2
4,285,826 - -2.2
4,285,933 + BPHYT_RS19100 0.12 -2.6
4,285,933 + BPHYT_RS19100 0.12 -2.5
4,285,934 - BPHYT_RS19100 0.12 -3.0
4,285,934 - BPHYT_RS19100 0.12 -3.5

Or see this region's nucleotide sequence